Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast Cancer
Abstract Targeted theranostic platform that integrates multi-modal imaging and therapeutic function is emerging as a promising strategy for earlier detection and precise treatment of cancer. Herein, we designed targeted gold-nanoshelled poly (lactic-co-glycolic acid) (PLGA) magnetic hybrid nanoparti...
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SpringerOpen
2019-08-01
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Series: | Nanoscale Research Letters |
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Online Access: | http://link.springer.com/article/10.1186/s11671-019-3053-4 |
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author | Qi Dong Hong Yang Caifeng Wan Dongdong Zheng Zhiguo Zhou Shaowei Xie Li Xu Jing Du Fenghua Li |
author_facet | Qi Dong Hong Yang Caifeng Wan Dongdong Zheng Zhiguo Zhou Shaowei Xie Li Xu Jing Du Fenghua Li |
author_sort | Qi Dong |
collection | DOAJ |
description | Abstract Targeted theranostic platform that integrates multi-modal imaging and therapeutic function is emerging as a promising strategy for earlier detection and precise treatment of cancer. Herein, we designed targeted gold-nanoshelled poly (lactic-co-glycolic acid) (PLGA) magnetic hybrid nanoparticles carrying anti-human epidermal growth factor receptor 2 (Her2) antibodies (Her2-GPH NPs) for dual-modal ultrasound (US)/magnetic resonance (MR) imaging and photothermal therapy of breast cancer. The agent was fabricated by coating gold nanoshell around PLGA nanoparticles co-loaded with perfluorooctyl bromide (PFOB) and superparamagnetic iron oxide nanoparticles (SPIOs), followed by conjugating with anti-Her2 antibodies. Cell-targeting studies demonstrated receptor-mediated specific binding of the agent to Her2-positive human breast cancer SKBR3 cells, and its binding rate was significantly higher than that of Her2-negative cells (P < 0.001). In vitro, the agent had capabilities for contrast-enhanced US imaging as well as T 2-weighted MR imaging with a relatively high relaxivity (r 2 = 441.47 mM−1 s−1). Furthermore, the Her2 functionalization of the agent prominently enhanced the US/MR molecular imaging effect of targeted cells by cell-specific binding. Live/dead cell assay and targeted photothermal cytotoxicity experiments confirmed that Her2-GPH NPs could serve as effective photoabsorbers to specifically induce SKBR3 cell death upon near-infrared laser irradiation. In summary, Her2-GPH NPs were demonstrated to be novel targeted theranostic agents with great potential to facilitate early non-invasive diagnosis and adjuvant therapy of breast cancer. |
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language | English |
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spelling | doaj.art-e42eca274b6345488903bf3e0762ea4b2023-09-03T01:43:42ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2019-08-0114111610.1186/s11671-019-3053-4Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast CancerQi Dong0Hong Yang1Caifeng Wan2Dongdong Zheng3Zhiguo Zhou4Shaowei Xie5Li Xu6Jing Du7Fenghua Li8Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Chemistry, College of Life and Environmental Science, Shanghai Normal UniversityDepartment of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Chemistry, College of Life and Environmental Science, Shanghai Normal UniversityDepartment of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityDepartment of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityAbstract Targeted theranostic platform that integrates multi-modal imaging and therapeutic function is emerging as a promising strategy for earlier detection and precise treatment of cancer. Herein, we designed targeted gold-nanoshelled poly (lactic-co-glycolic acid) (PLGA) magnetic hybrid nanoparticles carrying anti-human epidermal growth factor receptor 2 (Her2) antibodies (Her2-GPH NPs) for dual-modal ultrasound (US)/magnetic resonance (MR) imaging and photothermal therapy of breast cancer. The agent was fabricated by coating gold nanoshell around PLGA nanoparticles co-loaded with perfluorooctyl bromide (PFOB) and superparamagnetic iron oxide nanoparticles (SPIOs), followed by conjugating with anti-Her2 antibodies. Cell-targeting studies demonstrated receptor-mediated specific binding of the agent to Her2-positive human breast cancer SKBR3 cells, and its binding rate was significantly higher than that of Her2-negative cells (P < 0.001). In vitro, the agent had capabilities for contrast-enhanced US imaging as well as T 2-weighted MR imaging with a relatively high relaxivity (r 2 = 441.47 mM−1 s−1). Furthermore, the Her2 functionalization of the agent prominently enhanced the US/MR molecular imaging effect of targeted cells by cell-specific binding. Live/dead cell assay and targeted photothermal cytotoxicity experiments confirmed that Her2-GPH NPs could serve as effective photoabsorbers to specifically induce SKBR3 cell death upon near-infrared laser irradiation. In summary, Her2-GPH NPs were demonstrated to be novel targeted theranostic agents with great potential to facilitate early non-invasive diagnosis and adjuvant therapy of breast cancer.http://link.springer.com/article/10.1186/s11671-019-3053-4Theranostic agentAnti-Her2 antibodyUltrasound imagingMagnetic resonance imagingPhotothermal therapyBreast cancer |
spellingShingle | Qi Dong Hong Yang Caifeng Wan Dongdong Zheng Zhiguo Zhou Shaowei Xie Li Xu Jing Du Fenghua Li Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast Cancer Nanoscale Research Letters Theranostic agent Anti-Her2 antibody Ultrasound imaging Magnetic resonance imaging Photothermal therapy Breast cancer |
title | Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast Cancer |
title_full | Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast Cancer |
title_fullStr | Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast Cancer |
title_full_unstemmed | Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast Cancer |
title_short | Her2-Functionalized Gold-Nanoshelled Magnetic Hybrid Nanoparticles: a Theranostic Agent for Dual-Modal Imaging and Photothermal Therapy of Breast Cancer |
title_sort | her2 functionalized gold nanoshelled magnetic hybrid nanoparticles a theranostic agent for dual modal imaging and photothermal therapy of breast cancer |
topic | Theranostic agent Anti-Her2 antibody Ultrasound imaging Magnetic resonance imaging Photothermal therapy Breast cancer |
url | http://link.springer.com/article/10.1186/s11671-019-3053-4 |
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