Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageing
Caffeine (CAF) is a challenging natural bioactive compound with proven antiaging efficacy. However, being hydrophilic hampers its permeation through the skin. Our aim is to develop a novel CAF-loaded nano-cosmeceutical tool counteracting skin photoaging via improving CAF skin permeation using a bioa...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | International Journal of Pharmaceutics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590156723000142 |
| _version_ | 1797798703147253760 |
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| author | Manal A Elsheikh Passent M.E. Gaafar Mohamed A. Khattab Mohamed Kamal A. Helwah Mohamed H. Noureldin Haidy Abbas |
| author_facet | Manal A Elsheikh Passent M.E. Gaafar Mohamed A. Khattab Mohamed Kamal A. Helwah Mohamed H. Noureldin Haidy Abbas |
| author_sort | Manal A Elsheikh |
| collection | DOAJ |
| description | Caffeine (CAF) is a challenging natural bioactive compound with proven antiaging efficacy. However, being hydrophilic hampers its permeation through the skin. Our aim is to develop a novel CAF-loaded nano-cosmeceutical tool counteracting skin photoaging via improving CAF skin permeation using a bioactive nanocarrier. Caffeinated hyalurosomes are novel biocompatible antiaging nanoplatforms designed by immobilization of phospholipid vesicles with a hyaluronan polymer. Physicochemical properties of the selected hyalurosomes formulation showed nano-sized vesicles (210.10 ± 1.87 nm), with high zeta potential (−31.30 ± 1.19 mv), and high encapsulation efficiency (84.60 ± 1.05%). In vitro release results showed outstanding sustained release profile from caffeinated hyalurosomes compared to the CAF-loaded in conventional gel over 24 h. The in-vivo study revealed a photoprotective effect of caffeinated hyalurosomes, reflected from the intact and wrinkling-free skin. Results of biochemical analyses of oxidative stress, pro-inflammatory mediators, and anti-wrinkling markers further confirmed the efficacy of the prepared hyalurosomes compared to the CAF conventional gel. Finally, histopathological examination demonstrated normal histological structures of epidermal layers with minimal inflammatory cell infiltrates in the caffeinated hyalurosomes group compared to the positive control group. Conclusively, caffeinated hyalurosomes successfully achieved enhanced CAF loading and penetration into the skin besides the hydration effect of hyaluronan. Consequently, the developed delivery system presents a promising skin protection nano-platforms via the double effects of both hyaluronan and CAF, hence it guards against skin photodamage. |
| first_indexed | 2024-03-13T04:07:49Z |
| format | Article |
| id | doaj.art-e438cd2bcdfa411e82c6b61ea129bdd8 |
| institution | Directory Open Access Journal |
| issn | 2590-1567 |
| language | English |
| last_indexed | 2024-03-13T04:07:49Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Pharmaceutics: X |
| spelling | doaj.art-e438cd2bcdfa411e82c6b61ea129bdd82023-06-21T06:59:13ZengElsevierInternational Journal of Pharmaceutics: X2590-15672023-12-015100170Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageingManal A Elsheikh0Passent M.E. Gaafar1Mohamed A. Khattab2Mohamed Kamal A. Helwah3Mohamed H. Noureldin4Haidy Abbas5Department of Pharmaceutics, Faculty of Pharmacy, Damanhour University, Damanhour, EgyptDepartment of Pharmaceutics, Division of Pharmaceutical Sciences, College of Pharmacy, Arab Academy for Science, Technology and Maritime Transport, Alexandria, P.O. Box 1029, EgyptDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Cairo 12211, EgyptAvantic Pharmaceuticals, P.O. Box 12556, Giza, EgyptDepartment of Biochemistry, Division of Clinical and Biological Sciences, College of Pharmacy, Arab Academy for Science, Technology and Maritime Transport, Alexandria, P.O. Box 1029, EgyptDepartment of Pharmaceutics, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt; Corresponding author at: Department of Pharmaceutics, Faculty of Pharmacy, Damanhour University, Damanhour, El-Bahira, Egypt Post Office, P.O. Box 22511, Damanhour, Egypt.Caffeine (CAF) is a challenging natural bioactive compound with proven antiaging efficacy. However, being hydrophilic hampers its permeation through the skin. Our aim is to develop a novel CAF-loaded nano-cosmeceutical tool counteracting skin photoaging via improving CAF skin permeation using a bioactive nanocarrier. Caffeinated hyalurosomes are novel biocompatible antiaging nanoplatforms designed by immobilization of phospholipid vesicles with a hyaluronan polymer. Physicochemical properties of the selected hyalurosomes formulation showed nano-sized vesicles (210.10 ± 1.87 nm), with high zeta potential (−31.30 ± 1.19 mv), and high encapsulation efficiency (84.60 ± 1.05%). In vitro release results showed outstanding sustained release profile from caffeinated hyalurosomes compared to the CAF-loaded in conventional gel over 24 h. The in-vivo study revealed a photoprotective effect of caffeinated hyalurosomes, reflected from the intact and wrinkling-free skin. Results of biochemical analyses of oxidative stress, pro-inflammatory mediators, and anti-wrinkling markers further confirmed the efficacy of the prepared hyalurosomes compared to the CAF conventional gel. Finally, histopathological examination demonstrated normal histological structures of epidermal layers with minimal inflammatory cell infiltrates in the caffeinated hyalurosomes group compared to the positive control group. Conclusively, caffeinated hyalurosomes successfully achieved enhanced CAF loading and penetration into the skin besides the hydration effect of hyaluronan. Consequently, the developed delivery system presents a promising skin protection nano-platforms via the double effects of both hyaluronan and CAF, hence it guards against skin photodamage.http://www.sciencedirect.com/science/article/pii/S2590156723000142HyalurosomesCaffeineAnti-ageingUVBSkin deliveryCosmeceutical |
| spellingShingle | Manal A Elsheikh Passent M.E. Gaafar Mohamed A. Khattab Mohamed Kamal A. Helwah Mohamed H. Noureldin Haidy Abbas Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageing International Journal of Pharmaceutics: X Hyalurosomes Caffeine Anti-ageing UVB Skin delivery Cosmeceutical |
| title | Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageing |
| title_full | Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageing |
| title_fullStr | Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageing |
| title_full_unstemmed | Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageing |
| title_short | Dual-effects of caffeinated hyalurosomes as a nano-cosmeceutical gel counteracting UV-induced skin ageing |
| title_sort | dual effects of caffeinated hyalurosomes as a nano cosmeceutical gel counteracting uv induced skin ageing |
| topic | Hyalurosomes Caffeine Anti-ageing UVB Skin delivery Cosmeceutical |
| url | http://www.sciencedirect.com/science/article/pii/S2590156723000142 |
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