Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors
During the last decade, much progress has been made in developing protocols for the differentiation of human embryonic stem cells (hESCs) into a neural phenotype. The appropriate agent for cell therapy is neural precursors (NPs). Here, we demonstrate the derivation of highly enriched and expandable...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2010-04-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368909X484707b |
| _version_ | 1818063472628334592 |
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| author | Nataliya Kozubenko Karolina Turnovcova Miroslava Kapcalova Olena Butenko Miroslava Anderova Vendula Rusnakova Mikael Kubista Ales Hampl Pavla Jendelova Eva Sykova |
| author_facet | Nataliya Kozubenko Karolina Turnovcova Miroslava Kapcalova Olena Butenko Miroslava Anderova Vendula Rusnakova Mikael Kubista Ales Hampl Pavla Jendelova Eva Sykova |
| author_sort | Nataliya Kozubenko |
| collection | DOAJ |
| description | During the last decade, much progress has been made in developing protocols for the differentiation of human embryonic stem cells (hESCs) into a neural phenotype. The appropriate agent for cell therapy is neural precursors (NPs). Here, we demonstrate the derivation of highly enriched and expandable populations of proliferating NPs from the CCTL14 line of hESCs. These NPs could differentiate in vitro into functionally active neurons, as confirmed by immunohistochemical staining and electrophysiological analysis. Neural cells differentiated in vitro from hESCs exhibit broad cellular heterogeneity with respect to developmental stage and lineage specification. To analyze the population of the derived NPs, we used fluorescence-activated cell sorting (FACS) and characterized the expression of several pluripotent and neural markers, such as Nanog, SSEA-4, SSEA-1, TRA-1-60, CD24, CD133, CD56 (NCAM), β-III-tubulin, NF70, nestin, CD271 (NGFR), CD29, CD73, and CD105 during long-term propagation. The analyzed cells were used for transplantation into the injured rodent brain; the tumorigenicity of the transplanted cells was apparently eliminated following long-term culture. These results complete the characterization of the CCTL14 line of hESCs and provide a framework for developing cell selection strategies for neural cell-based therapies. |
| first_indexed | 2024-12-10T14:20:39Z |
| format | Article |
| id | doaj.art-e43906eae01c4464a44e6e9ae1d6dff6 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-10T14:20:39Z |
| publishDate | 2010-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-e43906eae01c4464a44e6e9ae1d6dff62022-12-22T01:45:13ZengSAGE PublishingCell Transplantation0963-68971555-38922010-04-011910.3727/096368909X484707bAnalysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural PrecursorsNataliya Kozubenko0Karolina Turnovcova1Miroslava Kapcalova2Olena Butenko3Miroslava Anderova4Vendula Rusnakova5Mikael Kubista6Ales Hampl7Pavla Jendelova8Eva Sykova9 Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, Prague, Czech Republic Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, Prague, Czech Republic Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, Prague, Czech Republic Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, Prague, Czech Republic Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, Czech Republic TATAA Biocenter, Lundberg Laboratory, Goteborg, Sweden Faculty of Medicine, Masaryk University, Brno, Czech Republic Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, Prague, Czech Republic Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, Prague, Czech RepublicDuring the last decade, much progress has been made in developing protocols for the differentiation of human embryonic stem cells (hESCs) into a neural phenotype. The appropriate agent for cell therapy is neural precursors (NPs). Here, we demonstrate the derivation of highly enriched and expandable populations of proliferating NPs from the CCTL14 line of hESCs. These NPs could differentiate in vitro into functionally active neurons, as confirmed by immunohistochemical staining and electrophysiological analysis. Neural cells differentiated in vitro from hESCs exhibit broad cellular heterogeneity with respect to developmental stage and lineage specification. To analyze the population of the derived NPs, we used fluorescence-activated cell sorting (FACS) and characterized the expression of several pluripotent and neural markers, such as Nanog, SSEA-4, SSEA-1, TRA-1-60, CD24, CD133, CD56 (NCAM), β-III-tubulin, NF70, nestin, CD271 (NGFR), CD29, CD73, and CD105 during long-term propagation. The analyzed cells were used for transplantation into the injured rodent brain; the tumorigenicity of the transplanted cells was apparently eliminated following long-term culture. These results complete the characterization of the CCTL14 line of hESCs and provide a framework for developing cell selection strategies for neural cell-based therapies.https://doi.org/10.3727/096368909X484707b |
| spellingShingle | Nataliya Kozubenko Karolina Turnovcova Miroslava Kapcalova Olena Butenko Miroslava Anderova Vendula Rusnakova Mikael Kubista Ales Hampl Pavla Jendelova Eva Sykova Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors Cell Transplantation |
| title | Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors |
| title_full | Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors |
| title_fullStr | Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors |
| title_full_unstemmed | Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors |
| title_short | Analysis of in Vitro and in Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors |
| title_sort | analysis of in vitro and in vivo characteristics of human embryonic stem cell derived neural precursors |
| url | https://doi.org/10.3727/096368909X484707b |
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