Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface
This study reports double-stacked planar bilayer lipid membranes (pBLMs) formed using a droplet contact method (DCM) for microfluidic formation with five-layered microchannels that have four micro guide pillars. pBLMs are valuable for analyzing membrane proteins and modeling cell membranes. Furtherm...
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| Language: | English |
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MDPI AG
2018-05-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/9/5/253 |
| _version_ | 1818034002498420736 |
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| author | Kan Shoji Ryuji Kawano |
| author_facet | Kan Shoji Ryuji Kawano |
| author_sort | Kan Shoji |
| collection | DOAJ |
| description | This study reports double-stacked planar bilayer lipid membranes (pBLMs) formed using a droplet contact method (DCM) for microfluidic formation with five-layered microchannels that have four micro guide pillars. pBLMs are valuable for analyzing membrane proteins and modeling cell membranes. Furthermore, multiple-pBLM systems have broadened the field of application such as electronic components, light-sensors, and batteries because of electrical characteristics of pBLMs and membrane proteins. Although multiple-stacked pBLMs have potential, the formation of multiple-pBLMs on a micrometer scale still faces challenges. In this study, we applied a DCM strategy to pBLM formation using microfluidic techniques and attempted to form double-stacked pBLMs in micro-meter scale. First, microchannels with micro pillars were designed via hydrodynamic simulations to form a five-layered flow with aqueous and lipid/oil solutions. Then, pBLMs were successfully formed by controlling the pumping pressure of the solutions and allowing contact between the two lipid monolayers. Finally, pore-forming proteins were reconstituted in the pBLMs, and ion current signals of nanopores were obtained as confirmed by electrical measurements, indicating that double-stacked pBLMs were successfully formed. The strategy for the double-stacked pBLM formation can be applied to highly integrated nanopore-based systems. |
| first_indexed | 2024-12-10T06:32:14Z |
| format | Article |
| id | doaj.art-e43f12b78fb24ed7b256b4026d8f6170 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-10T06:32:14Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-e43f12b78fb24ed7b256b4026d8f61702022-12-22T01:59:03ZengMDPI AGMicromachines2072-666X2018-05-019525310.3390/mi9050253mi9050253Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil InterfaceKan Shoji0Ryuji Kawano1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, JapanDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, JapanThis study reports double-stacked planar bilayer lipid membranes (pBLMs) formed using a droplet contact method (DCM) for microfluidic formation with five-layered microchannels that have four micro guide pillars. pBLMs are valuable for analyzing membrane proteins and modeling cell membranes. Furthermore, multiple-pBLM systems have broadened the field of application such as electronic components, light-sensors, and batteries because of electrical characteristics of pBLMs and membrane proteins. Although multiple-stacked pBLMs have potential, the formation of multiple-pBLMs on a micrometer scale still faces challenges. In this study, we applied a DCM strategy to pBLM formation using microfluidic techniques and attempted to form double-stacked pBLMs in micro-meter scale. First, microchannels with micro pillars were designed via hydrodynamic simulations to form a five-layered flow with aqueous and lipid/oil solutions. Then, pBLMs were successfully formed by controlling the pumping pressure of the solutions and allowing contact between the two lipid monolayers. Finally, pore-forming proteins were reconstituted in the pBLMs, and ion current signals of nanopores were obtained as confirmed by electrical measurements, indicating that double-stacked pBLMs were successfully formed. The strategy for the double-stacked pBLM formation can be applied to highly integrated nanopore-based systems.http://www.mdpi.com/2072-666X/9/5/253microfluidic lipid bilayer formationmultiple lipid bilayerswater-oil phase flow |
| spellingShingle | Kan Shoji Ryuji Kawano Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface Micromachines microfluidic lipid bilayer formation multiple lipid bilayers water-oil phase flow |
| title | Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface |
| title_full | Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface |
| title_fullStr | Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface |
| title_full_unstemmed | Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface |
| title_short | Microfluidic Formation of Double-Stacked Planar Bilayer Lipid Membranes by Controlling the Water-Oil Interface |
| title_sort | microfluidic formation of double stacked planar bilayer lipid membranes by controlling the water oil interface |
| topic | microfluidic lipid bilayer formation multiple lipid bilayers water-oil phase flow |
| url | http://www.mdpi.com/2072-666X/9/5/253 |
| work_keys_str_mv | AT kanshoji microfluidicformationofdoublestackedplanarbilayerlipidmembranesbycontrollingthewateroilinterface AT ryujikawano microfluidicformationofdoublestackedplanarbilayerlipidmembranesbycontrollingthewateroilinterface |