Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach
The objective of the current study was to compare the rumen inoculum of slaughtered cattle with the ruminal inoculum of cannulated cattle; additionally, determine its reliability in the in vitro analysis of ruminant feeds throughout a multivariate approach. Five male bovines (weight 320 ± 9.4 kg; me...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Ruminants |
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| Online Access: | https://www.mdpi.com/2673-933X/3/4/30 |
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| author | Henry Daniel Ruiz Alba Victor Guimarães Oliveira Lima Silvana Teixeira Carvalho Luis Carlos Vinhas Ítavo Luis Fernando Batista Pinto Paulo Luiz Souza Carneiro Ronaldo Lopes Oliveira |
| author_facet | Henry Daniel Ruiz Alba Victor Guimarães Oliveira Lima Silvana Teixeira Carvalho Luis Carlos Vinhas Ítavo Luis Fernando Batista Pinto Paulo Luiz Souza Carneiro Ronaldo Lopes Oliveira |
| author_sort | Henry Daniel Ruiz Alba |
| collection | DOAJ |
| description | The objective of the current study was to compare the rumen inoculum of slaughtered cattle with the ruminal inoculum of cannulated cattle; additionally, determine its reliability in the in vitro analysis of ruminant feeds throughout a multivariate approach. Five male bovines (weight 320 ± 9.4 kg; mean ± standard deviation) provided with ruminal cannula and between five and seven bovines slaughtered in slaughterhouse were used. The evaluations were carried out following a completely randomized design. The data obtained were subjected to different multivariate analyzes to determine the reliability of the ruminal inoculum of animals slaughtered in commercial slaughterhouses compared to that obtained from cannulated animals. The relative contribution indicated that the in vitro dry matter digestibility (IVDMD, 50.75%) and in vitro neutral detergent fiber digestibility (IVNDFD, 29.83%) analysis influence 80.13% of the results to determine the reliability of the ruminal inoculum from slaughtered cattle. Furthermore, it was determined that the first two principal components (IVNDFD and acetic acid production) are the ones that influence the results by 89.87%. The grouping of diets using the Tocher optimization method and the dendrogram shows the formation of six groups and two groups, respectively. The grouping shows that the ruminal inoculum source was not the limiting parameter in the evaluation. Rumen inoculum from cattle slaughtered in a commercial slaughterhouse (with unknown diet) has potential as an alternative for the in vitro analysis of cattle feed, provided that the lignin concentration in the diet is less than 35.5 g/kg DM. |
| first_indexed | 2024-03-08T20:23:33Z |
| format | Article |
| id | doaj.art-e4408bc3afdb4cd0bfbf57c249f8118f |
| institution | Directory Open Access Journal |
| issn | 2673-933X |
| language | English |
| last_indexed | 2024-03-08T20:23:33Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Ruminants |
| spelling | doaj.art-e4408bc3afdb4cd0bfbf57c249f8118f2023-12-22T14:39:41ZengMDPI AGRuminants2673-933X2023-11-013436037210.3390/ruminants3040030Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate ApproachHenry Daniel Ruiz Alba0Victor Guimarães Oliveira Lima1Silvana Teixeira Carvalho2Luis Carlos Vinhas Ítavo3Luis Fernando Batista Pinto4Paulo Luiz Souza Carneiro5Ronaldo Lopes Oliveira6Department of Animal Science, Universidade Federal da Bahia, Salvador 40170110, BA, BrazilDepartment of Animal Science, Universidade Federal da Bahia, Salvador 40170110, BA, BrazilDepartment of Animal Science, Universidade Estadual do Oeste do Paraná, Marechal Cândido Rondon 85960000, PR, BrazilDepartment of Animal Science, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070900, MS, BrazilDepartment of Animal Science, Universidade Federal da Bahia, Salvador 40170110, BA, BrazilDepartment of Animal Science, Universidade Estadual do Sudoeste da Bahia, Itapetinga 45700000, BA, BrazilDepartment of Animal Science, Universidade Federal da Bahia, Salvador 40170110, BA, BrazilThe objective of the current study was to compare the rumen inoculum of slaughtered cattle with the ruminal inoculum of cannulated cattle; additionally, determine its reliability in the in vitro analysis of ruminant feeds throughout a multivariate approach. Five male bovines (weight 320 ± 9.4 kg; mean ± standard deviation) provided with ruminal cannula and between five and seven bovines slaughtered in slaughterhouse were used. The evaluations were carried out following a completely randomized design. The data obtained were subjected to different multivariate analyzes to determine the reliability of the ruminal inoculum of animals slaughtered in commercial slaughterhouses compared to that obtained from cannulated animals. The relative contribution indicated that the in vitro dry matter digestibility (IVDMD, 50.75%) and in vitro neutral detergent fiber digestibility (IVNDFD, 29.83%) analysis influence 80.13% of the results to determine the reliability of the ruminal inoculum from slaughtered cattle. Furthermore, it was determined that the first two principal components (IVNDFD and acetic acid production) are the ones that influence the results by 89.87%. The grouping of diets using the Tocher optimization method and the dendrogram shows the formation of six groups and two groups, respectively. The grouping shows that the ruminal inoculum source was not the limiting parameter in the evaluation. Rumen inoculum from cattle slaughtered in a commercial slaughterhouse (with unknown diet) has potential as an alternative for the in vitro analysis of cattle feed, provided that the lignin concentration in the diet is less than 35.5 g/kg DM.https://www.mdpi.com/2673-933X/3/4/30alternative feedbyproductmultivariate analysisrumen contentruminal inoculumslaughter cattle |
| spellingShingle | Henry Daniel Ruiz Alba Victor Guimarães Oliveira Lima Silvana Teixeira Carvalho Luis Carlos Vinhas Ítavo Luis Fernando Batista Pinto Paulo Luiz Souza Carneiro Ronaldo Lopes Oliveira Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach Ruminants alternative feed byproduct multivariate analysis rumen content ruminal inoculum slaughter cattle |
| title | Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach |
| title_full | Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach |
| title_fullStr | Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach |
| title_full_unstemmed | Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach |
| title_short | Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach |
| title_sort | rumen content from slaughterhouse as an alternative inoculum source for in vitro analysis of feeds a multivariate approach |
| topic | alternative feed byproduct multivariate analysis rumen content ruminal inoculum slaughter cattle |
| url | https://www.mdpi.com/2673-933X/3/4/30 |
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