Improvement of Aptamer Affinity by Dimerization
To increase the affinities of aptamers for their targets, we designed an aptamerdimer for thrombin and VEGF. This design is based on the avidity of the antibody, whichenables the aptamer to connect easily since it is a single-strand nucleic acid. In this study,we connected a 15-mer thrombin-binding...
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| Format: | Article |
| Language: | English |
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MDPI AG
2008-02-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/8/2/1090/ |
| _version_ | 1798041959720288256 |
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| author | Kazunori Ikebukuro Koji Sode Ken-ichi Taira Hijiri Hasegawa |
| author_facet | Kazunori Ikebukuro Koji Sode Ken-ichi Taira Hijiri Hasegawa |
| author_sort | Kazunori Ikebukuro |
| collection | DOAJ |
| description | To increase the affinities of aptamers for their targets, we designed an aptamerdimer for thrombin and VEGF. This design is based on the avidity of the antibody, whichenables the aptamer to connect easily since it is a single-strand nucleic acid. In this study,we connected a 15-mer thrombin-binding aptamer with a 29-mer thrombin-binding aptamer.Each aptamer recognizes a different part of the thrombin molecule, and the aptamer dimerhas a Kd value which is 1/10 of that of the monomers from which it is composed. Also, thedesigned aptamer dimer has higher inhibitory activity than the reported (15-mer) thrombin-inhibiting aptamer. Additionally, we connected together two identical aptamers againstvascular endothelial growth factor (VEGF165), which is a homodimeric protein. As in thecase of the anti-thrombin aptamer, the dimeric anti-VEGF aptamer had a much lower Kd value than that of the monomer. This study demonstrated that the dimerization of aptamerseffectively improves the affinities of those aptamers for their targets. |
| first_indexed | 2024-04-11T22:28:51Z |
| format | Article |
| id | doaj.art-e442660507ae4e84890c51136db43bf8 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:28:51Z |
| publishDate | 2008-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-e442660507ae4e84890c51136db43bf82022-12-22T03:59:34ZengMDPI AGSensors1424-82202008-02-018210901098Improvement of Aptamer Affinity by DimerizationKazunori IkebukuroKoji SodeKen-ichi TairaHijiri HasegawaTo increase the affinities of aptamers for their targets, we designed an aptamerdimer for thrombin and VEGF. This design is based on the avidity of the antibody, whichenables the aptamer to connect easily since it is a single-strand nucleic acid. In this study,we connected a 15-mer thrombin-binding aptamer with a 29-mer thrombin-binding aptamer.Each aptamer recognizes a different part of the thrombin molecule, and the aptamer dimerhas a Kd value which is 1/10 of that of the monomers from which it is composed. Also, thedesigned aptamer dimer has higher inhibitory activity than the reported (15-mer) thrombin-inhibiting aptamer. Additionally, we connected together two identical aptamers againstvascular endothelial growth factor (VEGF165), which is a homodimeric protein. As in thecase of the anti-thrombin aptamer, the dimeric anti-VEGF aptamer had a much lower Kd value than that of the monomer. This study demonstrated that the dimerization of aptamerseffectively improves the affinities of those aptamers for their targets.http://www.mdpi.com/1424-8220/8/2/1090/Aptamerdimerizationavidity |
| spellingShingle | Kazunori Ikebukuro Koji Sode Ken-ichi Taira Hijiri Hasegawa Improvement of Aptamer Affinity by Dimerization Sensors Aptamer dimerization avidity |
| title | Improvement of Aptamer Affinity by Dimerization |
| title_full | Improvement of Aptamer Affinity by Dimerization |
| title_fullStr | Improvement of Aptamer Affinity by Dimerization |
| title_full_unstemmed | Improvement of Aptamer Affinity by Dimerization |
| title_short | Improvement of Aptamer Affinity by Dimerization |
| title_sort | improvement of aptamer affinity by dimerization |
| topic | Aptamer dimerization avidity |
| url | http://www.mdpi.com/1424-8220/8/2/1090/ |
| work_keys_str_mv | AT kazunoriikebukuro improvementofaptameraffinitybydimerization AT kojisode improvementofaptameraffinitybydimerization AT kenichitaira improvementofaptameraffinitybydimerization AT hijirihasegawa improvementofaptameraffinitybydimerization |