Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew
Developing effective and durable host plant resistance is crucial for controlling powdery mildew, a devastating disease caused by Blumeria graminis f. sp. tritici (Bgt). In the present study, we dissected the genetic basis of the adult plant resistance to powdery mildew using a recombinant inbred li...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1042399/full |
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| author | Hong Liu Guohao Han Tiantian Gu Yuli Jin Zhipeng Shi Lixian Xing Hanwen Yan Jing Wang Chenyang Hao Meicheng Zhao Diaoguo An Diaoguo An |
| author_facet | Hong Liu Guohao Han Tiantian Gu Yuli Jin Zhipeng Shi Lixian Xing Hanwen Yan Jing Wang Chenyang Hao Meicheng Zhao Diaoguo An Diaoguo An |
| author_sort | Hong Liu |
| collection | DOAJ |
| description | Developing effective and durable host plant resistance is crucial for controlling powdery mildew, a devastating disease caused by Blumeria graminis f. sp. tritici (Bgt). In the present study, we dissected the genetic basis of the adult plant resistance to powdery mildew using a recombinant inbred line (RIL) composed of 176 F9 RILs population derived from a cross between PuBing 3228 (P3228) and susceptible cultivar Gao 8901. P3228 exhibits stable adult-plant resistance to powdery mildew in the field over consecutive years. We identified two QTLs on chromosomes 7DS (QPm.cas-7D) and 1AL (QPm.cas-1A) contributed by P3228, and one QTL on 3DS (QPm.cas-3D) contributed by Gao 8901, which could explain 65.44%, 3.45%, and 2.18% of the phenotypic variances, respectively. By analyzing the annotated genes in the 1.168 Mb physical interval of the major QTL QPm.cas-7D, we locked a previously cloned adult-plant resistance gene Pm38 that was most probably the candidate gene of QPm.cas-7D. Sequence alignment analysis revealed that the candidate gene of QPm.cas-7D in P3228 was identical to the reported Pm38 sequence. Two haplotypes QPm-7D-R and QPm-7D-S were identified in the whole Pm38 genomic regions between P3228 and Gao 8901. To apply QPm.cas-7D in wheat breeding, we developed a kompetitive allele-specific PCR (KASP) marker Kasp5249 that is closely linked with these haplotypes. It is worth mentioning that the QPm-7D-R haplotype significantly decreased TKW and underwent negative selection for higher yields in China wheat breeding. In this study, we identified a major QTL QPm.cas-7D and revealed the relationship between its resistance and yield, which could be beneficial for further applications in wheat disease resistance and high-yield breeding. |
| first_indexed | 2024-04-11T07:31:09Z |
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| id | doaj.art-e44266a3269a4a34be95871840b6dd66 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T07:31:09Z |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-e44266a3269a4a34be95871840b6dd662022-12-22T04:36:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10423991042399Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildewHong Liu0Guohao Han1Tiantian Gu2Yuli Jin3Zhipeng Shi4Lixian Xing5Hanwen Yan6Jing Wang7Chenyang Hao8Meicheng Zhao9Diaoguo An10Diaoguo An11Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, ChinaInnovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaDeveloping effective and durable host plant resistance is crucial for controlling powdery mildew, a devastating disease caused by Blumeria graminis f. sp. tritici (Bgt). In the present study, we dissected the genetic basis of the adult plant resistance to powdery mildew using a recombinant inbred line (RIL) composed of 176 F9 RILs population derived from a cross between PuBing 3228 (P3228) and susceptible cultivar Gao 8901. P3228 exhibits stable adult-plant resistance to powdery mildew in the field over consecutive years. We identified two QTLs on chromosomes 7DS (QPm.cas-7D) and 1AL (QPm.cas-1A) contributed by P3228, and one QTL on 3DS (QPm.cas-3D) contributed by Gao 8901, which could explain 65.44%, 3.45%, and 2.18% of the phenotypic variances, respectively. By analyzing the annotated genes in the 1.168 Mb physical interval of the major QTL QPm.cas-7D, we locked a previously cloned adult-plant resistance gene Pm38 that was most probably the candidate gene of QPm.cas-7D. Sequence alignment analysis revealed that the candidate gene of QPm.cas-7D in P3228 was identical to the reported Pm38 sequence. Two haplotypes QPm-7D-R and QPm-7D-S were identified in the whole Pm38 genomic regions between P3228 and Gao 8901. To apply QPm.cas-7D in wheat breeding, we developed a kompetitive allele-specific PCR (KASP) marker Kasp5249 that is closely linked with these haplotypes. It is worth mentioning that the QPm-7D-R haplotype significantly decreased TKW and underwent negative selection for higher yields in China wheat breeding. In this study, we identified a major QTL QPm.cas-7D and revealed the relationship between its resistance and yield, which could be beneficial for further applications in wheat disease resistance and high-yield breeding.https://www.frontiersin.org/articles/10.3389/fpls.2022.1042399/fulladult plant resistancepowdery mildewPm38haplotypesmolecular markerscommon wheat |
| spellingShingle | Hong Liu Guohao Han Tiantian Gu Yuli Jin Zhipeng Shi Lixian Xing Hanwen Yan Jing Wang Chenyang Hao Meicheng Zhao Diaoguo An Diaoguo An Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew Frontiers in Plant Science adult plant resistance powdery mildew Pm38 haplotypes molecular markers common wheat |
| title | Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew |
| title_full | Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew |
| title_fullStr | Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew |
| title_full_unstemmed | Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew |
| title_short | Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew |
| title_sort | identification of the major qtl qpm cas 7d for adult plant resistance to wheat powdery mildew |
| topic | adult plant resistance powdery mildew Pm38 haplotypes molecular markers common wheat |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1042399/full |
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