A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology
A new biodiesel-like biofuel is obtained by the enzymatic ethanolysis reaction of sunflower oil with ethanol, in free solvent media, by using BIOLIPASE-R, a multipurpose alimentary additive from Biocon®-Spain that is a low cost lipase from a strain of Rhizopus oryzae. This biofuel is composed by two...
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| Format: | Article |
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MDPI AG
2014-05-01
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| Series: | Energies |
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| Online Access: | http://www.mdpi.com/1996-1073/7/5/3383 |
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| author | Carlos Luna Cristobal Verdugo Enrique D. Sancho Diego Luna Juan Calero Alejandro Posadillo Felipa M. Bautista Antonio A. Romero |
| author_facet | Carlos Luna Cristobal Verdugo Enrique D. Sancho Diego Luna Juan Calero Alejandro Posadillo Felipa M. Bautista Antonio A. Romero |
| author_sort | Carlos Luna |
| collection | DOAJ |
| description | A new biodiesel-like biofuel is obtained by the enzymatic ethanolysis reaction of sunflower oil with ethanol, in free solvent media, by using BIOLIPASE-R, a multipurpose alimentary additive from Biocon®-Spain that is a low cost lipase from a strain of Rhizopus oryzae. This biofuel is composed by two parts of fatty acid ethyl esters (FAEE) and one of monoglyceride (MG), which in this form integrates glycerol, through the application of the 1,3-selective lipases. Thus, this process minimizes waste generation and maximizes the efficiency of the process because no residual glycerol is produced. Response surface methodology (RSM) is employed to evaluate the main reaction parameters (reaction temperature, oil/ethanol ratio and pH) on the sunflower oil conversion. Water content and amount of lipase were also previously investigated. Regarding the results, we found that it operates optimally with a water content of the reaction medium of 0.15%, 0.05%–0.1% lipase by weight relative to the weight of oil used, 20 °C, volume ratio (mL/mL) oil/ethanol 12/3.5 and pH 12 (by addition of 50 µL of 10 N NaOH solution). These results have proven a very good efficiency of the biocatalyst in the studied selective process. |
| first_indexed | 2024-04-12T19:38:08Z |
| format | Article |
| id | doaj.art-e444607a088d46ffb0c8c94468eba7a9 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-12T19:38:08Z |
| publishDate | 2014-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-e444607a088d46ffb0c8c94468eba7a92022-12-22T03:19:10ZengMDPI AGEnergies1996-10732014-05-01753383339910.3390/en7053383en7053383A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface MethodologyCarlos Luna0Cristobal Verdugo1Enrique D. Sancho2Diego Luna3Juan Calero4Alejandro Posadillo5Felipa M. Bautista6Antonio A. Romero7Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, SpainLaboratorio de Estudios Cristalográficos, IACT, CSIC-University of Granada, Avenida de las Palmeras 4, 18100 Armilla, Granada, SpainDepartment of Microbiology, University of Córdoba, Campus de Rabanales, Ed. Severo Ochoa, 14014 Córdoba, SpainDepartment of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, SpainDepartment of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, SpainSeneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, SpainDepartment of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, SpainDepartment of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, SpainA new biodiesel-like biofuel is obtained by the enzymatic ethanolysis reaction of sunflower oil with ethanol, in free solvent media, by using BIOLIPASE-R, a multipurpose alimentary additive from Biocon®-Spain that is a low cost lipase from a strain of Rhizopus oryzae. This biofuel is composed by two parts of fatty acid ethyl esters (FAEE) and one of monoglyceride (MG), which in this form integrates glycerol, through the application of the 1,3-selective lipases. Thus, this process minimizes waste generation and maximizes the efficiency of the process because no residual glycerol is produced. Response surface methodology (RSM) is employed to evaluate the main reaction parameters (reaction temperature, oil/ethanol ratio and pH) on the sunflower oil conversion. Water content and amount of lipase were also previously investigated. Regarding the results, we found that it operates optimally with a water content of the reaction medium of 0.15%, 0.05%–0.1% lipase by weight relative to the weight of oil used, 20 °C, volume ratio (mL/mL) oil/ethanol 12/3.5 and pH 12 (by addition of 50 µL of 10 N NaOH solution). These results have proven a very good efficiency of the biocatalyst in the studied selective process.http://www.mdpi.com/1996-1073/7/5/3383biodieselRhizopus oryzae lipase (ROL)BIOLIPASE-Rselective transesterificationethanolysisecodieselsunflower oilglycerol |
| spellingShingle | Carlos Luna Cristobal Verdugo Enrique D. Sancho Diego Luna Juan Calero Alejandro Posadillo Felipa M. Bautista Antonio A. Romero A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology Energies biodiesel Rhizopus oryzae lipase (ROL) BIOLIPASE-R selective transesterification ethanolysis ecodiesel sunflower oil glycerol |
| title | A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology |
| title_full | A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology |
| title_fullStr | A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology |
| title_full_unstemmed | A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology |
| title_short | A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae, Optimized by Response Surface Methodology |
| title_sort | biofuel similar to biodiesel obtained by using a lipase from rhizopus oryzae optimized by response surface methodology |
| topic | biodiesel Rhizopus oryzae lipase (ROL) BIOLIPASE-R selective transesterification ethanolysis ecodiesel sunflower oil glycerol |
| url | http://www.mdpi.com/1996-1073/7/5/3383 |
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