FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA
Objective To research the expression levels of FEN1 and PCNA in carcinoma ⁃ associated fibroblasts (CAFs) and analyze their correlation. Methods Fresh specimens of oral squamous cell carcinoma tissues and normal oral mucosal tissues excised during oral and maxillofacial plastic surgery were collec...
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2018-06-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2018.06.003 |
| _version_ | 1811278576280928256 |
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| author | ZHOU Hongmei |
| author_facet | ZHOU Hongmei |
| author_sort | ZHOU Hongmei |
| collection | DOAJ |
| description | Objective To research the expression levels of FEN1 and PCNA in carcinoma ⁃ associated fibroblasts
(CAFs) and analyze their correlation. Methods Fresh specimens of oral squamous cell carcinoma tissues and normal
oral mucosal tissues excised during oral and maxillofacial plastic surgery were collected. Primary oral CAFs and normal
fibroblasts (NFs) were obtained by tissue culture, identified by immunocytochemistry and divided into the CAF and NF
groups. Western blot and quantitative real ⁃time PCR were used to detect the protein and mRNA expression levels of
both FEN1 and PCNA in the oral CAFs and NFs. The correlation between FEN1 and PCNA expression in oral CAFs
was analyzed. Results Oral CAFs and oral NFs were successfully cultured and identified from 12 samples. Both the protein and mRNA expression levels of FEN1 and PCNA were higher in the oral CAFs than NFs, but there were no sig⁃
nificant differences (P > 0.05). In the oral CAFs, the linear correlation coefficient between FEN1 and PCNA was 0.677
(P = 0.016) at the mRNA level, indicating a strong positive correlation; however, at the protein level, no correlation was
found (P > 0.05). Conclusion In primary cultured oral CAFs and NFs, there were no significant differences in the
FEN1 and PCNA protein and mRNA expression levels. However, in the CAFs, the mRNA levels of FEN1 and PCNA
had a strong positive correlation. The relationship and the regulatory mechanism of the two genes require further study. |
| first_indexed | 2024-04-13T00:38:19Z |
| format | Article |
| id | doaj.art-e4452a97bf4d4ae7b63955e6afe89d87 |
| institution | Directory Open Access Journal |
| issn | 2096-1456 2096-1456 |
| language | zho |
| last_indexed | 2024-04-13T00:38:19Z |
| publishDate | 2018-06-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-e4452a97bf4d4ae7b63955e6afe89d872022-12-22T03:10:16ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562096-14562018-06-0126635435910.12016/j.issn.2096⁃1456.2018.06.003FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA ZHOU Hongmei0State Key Laboratory of Oral Diseas⁃ es, Department of Oral Medicine, West China Hospital of Stomatology, Sichuan UniversityObjective To research the expression levels of FEN1 and PCNA in carcinoma ⁃ associated fibroblasts (CAFs) and analyze their correlation. Methods Fresh specimens of oral squamous cell carcinoma tissues and normal oral mucosal tissues excised during oral and maxillofacial plastic surgery were collected. Primary oral CAFs and normal fibroblasts (NFs) were obtained by tissue culture, identified by immunocytochemistry and divided into the CAF and NF groups. Western blot and quantitative real ⁃time PCR were used to detect the protein and mRNA expression levels of both FEN1 and PCNA in the oral CAFs and NFs. The correlation between FEN1 and PCNA expression in oral CAFs was analyzed. Results Oral CAFs and oral NFs were successfully cultured and identified from 12 samples. Both the protein and mRNA expression levels of FEN1 and PCNA were higher in the oral CAFs than NFs, but there were no sig⁃ nificant differences (P > 0.05). In the oral CAFs, the linear correlation coefficient between FEN1 and PCNA was 0.677 (P = 0.016) at the mRNA level, indicating a strong positive correlation; however, at the protein level, no correlation was found (P > 0.05). Conclusion In primary cultured oral CAFs and NFs, there were no significant differences in the FEN1 and PCNA protein and mRNA expression levels. However, in the CAFs, the mRNA levels of FEN1 and PCNA had a strong positive correlation. The relationship and the regulatory mechanism of the two genes require further study.http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2018.06.003Carcinoma ⁃ associated fibroblastsFlap endonuclease ⁃ 1Proliferating cell nuclear antigenEx⁃ pression levelOral cavity |
| spellingShingle | ZHOU Hongmei FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA 口腔疾病防治 Carcinoma ⁃ associated fibroblasts Flap endonuclease ⁃ 1 Proliferating cell nuclear antigen Ex⁃ pression level Oral cavity |
| title | FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA |
| title_full | FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA |
| title_fullStr | FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA |
| title_full_unstemmed | FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA |
| title_short | FEN1 expression in oral carcinoma⁃associated fibroblasts and its relationship with PCNA |
| title_sort | fen1 expression in oral carcinoma⁃associated fibroblasts and its relationship with pcna |
| topic | Carcinoma ⁃ associated fibroblasts Flap endonuclease ⁃ 1 Proliferating cell nuclear antigen Ex⁃ pression level Oral cavity |
| url | http://www.kqjbfz.com/EN/10.12016/j.issn.2096-1456.2018.06.003 |
| work_keys_str_mv | AT zhouhongmei fen1expressioninoralcarcinomaassociatedfibroblastsanditsrelationshipwithpcna |