A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency
The effect of iron, manganese, phosphorus and nitrogen on growth and lipid synthesis of the microalgae <i>Nannochloropsis oceanica</i> CCMP1779, as well as their impact on the magnetic harvesting efficiency, are examined under their depriving cell culture conditions. Herein, it is demons...
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MDPI AG
2020-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/5/660 |
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| author | Maria G. Savvidou Elenitsa Boli Dimitrios Logothetis Theopisti Lymperopoulou Angelo Ferraro Vasiliki Louli Diomi Mamma Dimitris Kekos Kostis Magoulas Fragiskos N. Kolisis |
| author_facet | Maria G. Savvidou Elenitsa Boli Dimitrios Logothetis Theopisti Lymperopoulou Angelo Ferraro Vasiliki Louli Diomi Mamma Dimitris Kekos Kostis Magoulas Fragiskos N. Kolisis |
| author_sort | Maria G. Savvidou |
| collection | DOAJ |
| description | The effect of iron, manganese, phosphorus and nitrogen on growth and lipid synthesis of the microalgae <i>Nannochloropsis oceanica</i> CCMP1779, as well as their impact on the magnetic harvesting efficiency, are examined under their depriving cell culture conditions. Herein, it is demonstrated that nitrogen and manganese depletion primarily reduced cell growth while phosphorus and iron restriction led to higher dry biomass. Subsequently, the role of those nutrients on fatty acids profile was examined. Phosphorus and nitrogen restriction resulted in lower and higher lipid content, respectively. High amounts of polyunsaturated fatty acids like eicosapentaenoic acid are produced under iron and manganese depletion. Phosphorus deprivation favors monounsaturated fatty acids such as C18:1 and C16:1, while nitrogen restriction favors saturated fatty acid production like C14:0, C16:0 and C18:0. Since the presence/absence of macro- and micro-elements may affect the overall electrostatic charges on the outmost microalgae surface, it was also analyzed how these elements affect the magnetic harvesting efficiency. Results showed that phosphorus deprivation led to the best magnetic harvesting efficiency of <i>N. oceanica</i> cells (93%) as compared to other nutrient starvation as well as standard medium. |
| first_indexed | 2024-03-10T19:39:07Z |
| format | Article |
| id | doaj.art-e445784125354314acd660a4d7754bd0 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T19:39:07Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-e445784125354314acd660a4d7754bd02023-11-20T01:28:46ZengMDPI AGPlants2223-77472020-05-019566010.3390/plants9050660A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting EfficiencyMaria G. Savvidou0Elenitsa Boli1Dimitrios Logothetis2Theopisti Lymperopoulou3Angelo Ferraro4Vasiliki Louli5Diomi Mamma6Dimitris Kekos7Kostis Magoulas8Fragiskos N. Kolisis9Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceLaboratory of Thermodynamics and Transport Phenomena, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceLaboratory of Thermodynamics and Transport Phenomena, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceEnvironment and Quality of Life Center, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceLaboratory of Thermodynamics and Transport Phenomena, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceLaboratory of Thermodynamics and Transport Phenomena, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceBiotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str, Zografou Campus, 15780 Athens, GreeceThe effect of iron, manganese, phosphorus and nitrogen on growth and lipid synthesis of the microalgae <i>Nannochloropsis oceanica</i> CCMP1779, as well as their impact on the magnetic harvesting efficiency, are examined under their depriving cell culture conditions. Herein, it is demonstrated that nitrogen and manganese depletion primarily reduced cell growth while phosphorus and iron restriction led to higher dry biomass. Subsequently, the role of those nutrients on fatty acids profile was examined. Phosphorus and nitrogen restriction resulted in lower and higher lipid content, respectively. High amounts of polyunsaturated fatty acids like eicosapentaenoic acid are produced under iron and manganese depletion. Phosphorus deprivation favors monounsaturated fatty acids such as C18:1 and C16:1, while nitrogen restriction favors saturated fatty acid production like C14:0, C16:0 and C18:0. Since the presence/absence of macro- and micro-elements may affect the overall electrostatic charges on the outmost microalgae surface, it was also analyzed how these elements affect the magnetic harvesting efficiency. Results showed that phosphorus deprivation led to the best magnetic harvesting efficiency of <i>N. oceanica</i> cells (93%) as compared to other nutrient starvation as well as standard medium.https://www.mdpi.com/2223-7747/9/5/660starvationfatty acids profilemicroalgaemagnetic nanoparticlesculture medium |
| spellingShingle | Maria G. Savvidou Elenitsa Boli Dimitrios Logothetis Theopisti Lymperopoulou Angelo Ferraro Vasiliki Louli Diomi Mamma Dimitris Kekos Kostis Magoulas Fragiskos N. Kolisis A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency Plants starvation fatty acids profile microalgae magnetic nanoparticles culture medium |
| title | A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency |
| title_full | A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency |
| title_fullStr | A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency |
| title_full_unstemmed | A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency |
| title_short | A Study on the Effect of Macro- and Micro- Nutrients on <i>Nannochloropsis oceanica</i> Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency |
| title_sort | study on the effect of macro and micro nutrients on i nannochloropsis oceanica i growth fatty acid composition and magnetic harvesting efficiency |
| topic | starvation fatty acids profile microalgae magnetic nanoparticles culture medium |
| url | https://www.mdpi.com/2223-7747/9/5/660 |
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