The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development
Summary Xanthomonas campestris pv. campestris (Xcc) is a vascular pathogen that invades the xylem of Brassica crops. Current chemical and antibiotics‐based control measures for this bacterium are unsustainable and inefficient. After establishing a representative collection of Xcc strains, we isolate...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-06-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14004 |
| _version_ | 1818213935734587392 |
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| author | Dominique Holtappels Kiandro J. Fortuna Lauren Moons Nand Broeckaert Léon E. Bäcker Sofie Venneman Sofie Rombouts Louis Lippens Steve Baeyen Sabien Pollet Jean‐Paul Noben Frank Oechslin Marta Vallino Abram Aertsen Martine Maes Johan Van Vaerenbergh Rob Lavigne Jeroen Wagemans |
| author_facet | Dominique Holtappels Kiandro J. Fortuna Lauren Moons Nand Broeckaert Léon E. Bäcker Sofie Venneman Sofie Rombouts Louis Lippens Steve Baeyen Sabien Pollet Jean‐Paul Noben Frank Oechslin Marta Vallino Abram Aertsen Martine Maes Johan Van Vaerenbergh Rob Lavigne Jeroen Wagemans |
| author_sort | Dominique Holtappels |
| collection | DOAJ |
| description | Summary Xanthomonas campestris pv. campestris (Xcc) is a vascular pathogen that invades the xylem of Brassica crops. Current chemical and antibiotics‐based control measures for this bacterium are unsustainable and inefficient. After establishing a representative collection of Xcc strains, we isolated and characterized bacteriophages from two clades of phages to assess their potential in phage‐based biocontrol. The most promising phages, FoX2 and FoX6, specifically recognize (lipo) polysaccharides, associated with the wxc gene cluster, on the surface of the bacterial cell wall. Next, we determined and optimized the applicability of FoX2 and FoX6 in an array of complementary bioassays, ranging from seed decontamination to irrigation‐ and spray‐based applications. Here, an irrigation‐based application showed promising results. In a final proof‐of‐concept, a CaCl2‐formulated phage cocktail was shown to control the outbreak of Xcc in the open field. This comprehensive approach illustrates the potential of phage biocontrol of black rot disease in Brassica and serves as a reference for the broader implementation of phage biocontrol in integrated pest management strategies. |
| first_indexed | 2024-12-12T06:12:12Z |
| format | Article |
| id | doaj.art-e44855ec774241f6ab6a8467e03d1c04 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-12T06:12:12Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-e44855ec774241f6ab6a8467e03d1c042022-12-22T00:35:09ZengWileyMicrobial Biotechnology1751-79152022-06-011561762178210.1111/1751-7915.14004The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease developmentDominique Holtappels0Kiandro J. Fortuna1Lauren Moons2Nand Broeckaert3Léon E. Bäcker4Sofie Venneman5Sofie Rombouts6Louis Lippens7Steve Baeyen8Sabien Pollet9Jean‐Paul Noben10Frank Oechslin11Marta Vallino12Abram Aertsen13Martine Maes14Johan Van Vaerenbergh15Rob Lavigne16Jeroen Wagemans17Laboratory of Gene Technology KU Leuven Leuven BelgiumLaboratory of Gene Technology KU Leuven Leuven BelgiumLaboratory of Gene Technology KU Leuven Leuven BelgiumLaboratory of Gene Technology KU Leuven Leuven BelgiumLaboratory of Food Microbiology KU Leuven Leuven BelgiumProefstation voor de groenteteelt Sint‐Katelijne‐Waver BelgiumLaboratory of Gene Technology KU Leuven Leuven BelgiumProvinciaal Proefcentrum voor de Groenteteelt Oost‐Vlaanderen Kruishoutem BelgiumFlanders Research Institute for Agriculture, Fisheries and Food Ghent BelgiumInagro Beitem BelgiumBiomedical Research Institute and Transnational University Limburg Hasselt University Hasselt BelgiumDepartment of Fundamental Microbiology University of Lausanne Lausanne SwitzerlandInstitute for Sustainable Plant Protection National Research Counsil of Italy Turin ItalyLaboratory of Food Microbiology KU Leuven Leuven BelgiumIndependent Researcher Ghent BelgiumFlanders Research Institute for Agriculture, Fisheries and Food Ghent BelgiumLaboratory of Gene Technology KU Leuven Leuven BelgiumLaboratory of Gene Technology KU Leuven Leuven BelgiumSummary Xanthomonas campestris pv. campestris (Xcc) is a vascular pathogen that invades the xylem of Brassica crops. Current chemical and antibiotics‐based control measures for this bacterium are unsustainable and inefficient. After establishing a representative collection of Xcc strains, we isolated and characterized bacteriophages from two clades of phages to assess their potential in phage‐based biocontrol. The most promising phages, FoX2 and FoX6, specifically recognize (lipo) polysaccharides, associated with the wxc gene cluster, on the surface of the bacterial cell wall. Next, we determined and optimized the applicability of FoX2 and FoX6 in an array of complementary bioassays, ranging from seed decontamination to irrigation‐ and spray‐based applications. Here, an irrigation‐based application showed promising results. In a final proof‐of‐concept, a CaCl2‐formulated phage cocktail was shown to control the outbreak of Xcc in the open field. This comprehensive approach illustrates the potential of phage biocontrol of black rot disease in Brassica and serves as a reference for the broader implementation of phage biocontrol in integrated pest management strategies.https://doi.org/10.1111/1751-7915.14004 |
| spellingShingle | Dominique Holtappels Kiandro J. Fortuna Lauren Moons Nand Broeckaert Léon E. Bäcker Sofie Venneman Sofie Rombouts Louis Lippens Steve Baeyen Sabien Pollet Jean‐Paul Noben Frank Oechslin Marta Vallino Abram Aertsen Martine Maes Johan Van Vaerenbergh Rob Lavigne Jeroen Wagemans The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development Microbial Biotechnology |
| title | The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development |
| title_full | The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development |
| title_fullStr | The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development |
| title_full_unstemmed | The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development |
| title_short | The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development |
| title_sort | potential of bacteriophages to control xanthomonas campestris pv campestris at different stages of disease development |
| url | https://doi.org/10.1111/1751-7915.14004 |
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