Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts
Abstract Objective Erucin (ERN), an isothiocyanate, is derived from the vegetable arugula. Although ERN has antitumor and antioxidant activity, the effect of ERN on osteoclast and osteoblast differentiation is not well documented. In this study, we evaluated the effects of ERN on osteoclast and oste...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-022-05988-3 |
| _version_ | 1828871786682384384 |
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| author | Tomohiro Takagi Hirofumi Inoue Shungo Fujii Nobuyuki Takahashi Mariko Uehara |
| author_facet | Tomohiro Takagi Hirofumi Inoue Shungo Fujii Nobuyuki Takahashi Mariko Uehara |
| author_sort | Tomohiro Takagi |
| collection | DOAJ |
| description | Abstract Objective Erucin (ERN), an isothiocyanate, is derived from the vegetable arugula. Although ERN has antitumor and antioxidant activity, the effect of ERN on osteoclast and osteoblast differentiation is not well documented. In this study, we evaluated the effects of ERN on osteoclast and osteoblast differentiation in vitro. Results ERN significantly reduced the formation of 1α,25(OH)2D3-induced tartrate-resistant acid phosphatase (TRAP)-positive cells at non-cytotoxic concentrations. Furthermore, ERN downregulated the mRNA expression of osteoclast-associated genes, such as nuclear factor of activated T cells cytoplasmic-1, TRAP, and cathepsin K. In addition, ERN suppressed mRNA expression of dendritic cell specific transmembrane protein (DC-STAMP), which encodes cell–cell fusion. However, ERN did not affect mineralization by osteoblasts. Thus, our data suggest that ERN may attenuate osteoclastic bone resorption by inhibiting multinucleation of mononuclear pre-osteoclasts and by suppressing mRNA expression of DC-STAMP in bone marrow cells without influencing mineralization by osteoblasts. |
| first_indexed | 2024-12-13T06:40:38Z |
| format | Article |
| id | doaj.art-e4487edba674456e89c6ef374b98e641 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-13T06:40:38Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-e4487edba674456e89c6ef374b98e6412022-12-21T23:56:26ZengBMCBMC Research Notes1756-05002022-03-011511610.1186/s13104-022-05988-3Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblastsTomohiro Takagi0Hirofumi Inoue1Shungo Fujii2Nobuyuki Takahashi3Mariko Uehara4Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of AgricultureDepartment of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of AgricultureDepartment of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of AgricultureDepartment of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of AgricultureDepartment of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of AgricultureAbstract Objective Erucin (ERN), an isothiocyanate, is derived from the vegetable arugula. Although ERN has antitumor and antioxidant activity, the effect of ERN on osteoclast and osteoblast differentiation is not well documented. In this study, we evaluated the effects of ERN on osteoclast and osteoblast differentiation in vitro. Results ERN significantly reduced the formation of 1α,25(OH)2D3-induced tartrate-resistant acid phosphatase (TRAP)-positive cells at non-cytotoxic concentrations. Furthermore, ERN downregulated the mRNA expression of osteoclast-associated genes, such as nuclear factor of activated T cells cytoplasmic-1, TRAP, and cathepsin K. In addition, ERN suppressed mRNA expression of dendritic cell specific transmembrane protein (DC-STAMP), which encodes cell–cell fusion. However, ERN did not affect mineralization by osteoblasts. Thus, our data suggest that ERN may attenuate osteoclastic bone resorption by inhibiting multinucleation of mononuclear pre-osteoclasts and by suppressing mRNA expression of DC-STAMP in bone marrow cells without influencing mineralization by osteoblasts.https://doi.org/10.1186/s13104-022-05988-3ErucinIsothiocyanateOsteoclast differentiationOsteoclast cell fusionDC-STAMP |
| spellingShingle | Tomohiro Takagi Hirofumi Inoue Shungo Fujii Nobuyuki Takahashi Mariko Uehara Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts BMC Research Notes Erucin Isothiocyanate Osteoclast differentiation Osteoclast cell fusion DC-STAMP |
| title | Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts |
| title_full | Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts |
| title_fullStr | Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts |
| title_full_unstemmed | Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts |
| title_short | Erucin inhibits osteoclast formation via suppressing cell–cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts |
| title_sort | erucin inhibits osteoclast formation via suppressing cell cell fusion molecule dc stamp without influencing mineralization by osteoblasts |
| topic | Erucin Isothiocyanate Osteoclast differentiation Osteoclast cell fusion DC-STAMP |
| url | https://doi.org/10.1186/s13104-022-05988-3 |
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