Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins
Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/418 |
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| author | Mikhail Oliveira Leastro Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús Á. Sánchez-Navarro |
| author_facet | Mikhail Oliveira Leastro Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús Á. Sánchez-Navarro |
| author_sort | Mikhail Oliveira Leastro |
| collection | DOAJ |
| description | Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed. |
| first_indexed | 2024-03-09T00:49:05Z |
| format | Article |
| id | doaj.art-e44bf21d60df47448fcd7747a0983f25 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T00:49:05Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e44bf21d60df47448fcd7747a0983f252023-12-11T17:22:46ZengMDPI AGMicroorganisms2076-26072021-02-019241810.3390/microorganisms9020418Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid ProteinsMikhail Oliveira Leastro0Juliana Freitas-Astúa1Elliot Watanabe Kitajima2Vicente Pallás3Jesús Á. Sánchez-Navarro4Unidade Laboratorial de Referência em Biologia Molecular Aplicada, Instituto Biológico, São Paulo, SP 04014-002, BrazilUnidade Laboratorial de Referência em Biologia Molecular Aplicada, Instituto Biológico, São Paulo, SP 04014-002, BrazilDepartamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP 13418-900, BrazilInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas (CSIC), 46022 Valencia, SpainInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas (CSIC), 46022 Valencia, SpainAlthough citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed.https://www.mdpi.com/2076-2607/9/2/418membrane associationtopologycilevirusmovement proteinp29 capsid protein |
| spellingShingle | Mikhail Oliveira Leastro Juliana Freitas-Astúa Elliot Watanabe Kitajima Vicente Pallás Jesús Á. Sánchez-Navarro Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins Microorganisms membrane association topology cilevirus movement protein p29 capsid protein |
| title | Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins |
| title_full | Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins |
| title_fullStr | Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins |
| title_full_unstemmed | Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins |
| title_short | Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins |
| title_sort | membrane association and topology of citrus leprosis virus c2 movement and capsid proteins |
| topic | membrane association topology cilevirus movement protein p29 capsid protein |
| url | https://www.mdpi.com/2076-2607/9/2/418 |
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