Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells Ferroptosis
Jia Chen,1– 3 Xiaoyu Deng,1– 3 Hansheng Xie,1– 3 Caiyun Wang,1– 3 Jiefeng Huang,1– 3 Ningfang Lian1– 3 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 2Fujian Provincial Sleep-Disordered Breathing...
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Dove Medical Press
2024-02-01
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| Series: | International Journal of COPD |
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| Online Access: | https://www.dovepress.com/circular-rna0025843-alleviated-cigarette-smoke-extract-induced-broncho-peer-reviewed-fulltext-article-COPD |
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| author | Chen J Deng X Xie H Wang C Huang J Lian N |
| author_facet | Chen J Deng X Xie H Wang C Huang J Lian N |
| author_sort | Chen J |
| collection | DOAJ |
| description | Jia Chen,1– 3 Xiaoyu Deng,1– 3 Hansheng Xie,1– 3 Caiyun Wang,1– 3 Jiefeng Huang,1– 3 Ningfang Lian1– 3 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 2Fujian Provincial Sleep-Disordered Breathing Clinic Center, Institute of Respiratory Disease, Fujian Medical University, Fuzhou, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, People’s Republic of ChinaCorrespondence: Ningfang Lian, Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20, Chazhong Road, Taijiang District, Fuzhou, Fujian Province, 350005, People’s Republic of China, Tel +86-591-87981698, Email 1533532863@qq.comPurpose: Circular RNA (circRNA) plays an important role in various biological processes. However, their functions in cigarette smoke extract (CSE) induced human normal lung epithelial cells (BEAS-2B) injury remain vague. The study aimed to explore circRNA expression profiles and reveal their potential roles in CSE-treated BEAS-2B cells.Methods: 5% CSE exposure for 24 hours were used to build the BEAS-2B cells ferroptosis model. Differentially expressed circRNAs (DECs) were identified by next-generation RNA sequencing. Six randomly selected DECs were validated via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Ontology (GO) analysis were conducted to clarify the potential functions of the DECs. Furthermore, the role of hsa_circ_0025843 in CSE-related BEAS-2B cells ferroptosis was confirmed.Results: 5% CSE exposure induced BEAS-2B cells ferroptosis. Fifty-one up-regulated cirRNAs and 80 down-regulated circRNAs were revealed in CSE-treated BEAS-2B cells. Hsa_circ_0003461, hsa_circ_0007548, hsa_circ_0025843, hsa_circ_0068896, hsa_circ_0005832, and hsa_circ_0053378 were selected randomly to validate the reliability of next-generation RNA sequencing by qRT-PCR. After KEGG pathway analysis, DECs were found to participate in the process of EGFR tyrosine kinase inhibitor resistance and glycerophospholipid metabolism. The knockdown of hsa_circ_0025843 significantly alleviated CSE-induced BEAS-2B cells ferroptosis.Conclusion: The study indicated the circRNA expression profiles in CSE-treated BEAS-2B cells. Hsa_circ_0025843 alleviated CSE induced BEAS-2B cells ferroptosis, which might be a potential therapeutic target of CSE related lung injury.Keywords: cigarette smoke extract, Circular RNA, next-generation RNA sequencing, lung injury, ferroptosis |
| first_indexed | 2024-03-08T06:09:01Z |
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| language | English |
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| series | International Journal of COPD |
| spelling | doaj.art-e44f52efc81f453082af44031248b1ae2024-02-04T17:43:33ZengDove Medical PressInternational Journal of COPD1178-20052024-02-01Volume 1936337490200Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells FerroptosisChen JDeng XXie HWang CHuang JLian NJia Chen,1– 3 Xiaoyu Deng,1– 3 Hansheng Xie,1– 3 Caiyun Wang,1– 3 Jiefeng Huang,1– 3 Ningfang Lian1– 3 1Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 2Fujian Provincial Sleep-Disordered Breathing Clinic Center, Institute of Respiratory Disease, Fujian Medical University, Fuzhou, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, People’s Republic of ChinaCorrespondence: Ningfang Lian, Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Fujian Medical University, No. 20, Chazhong Road, Taijiang District, Fuzhou, Fujian Province, 350005, People’s Republic of China, Tel +86-591-87981698, Email 1533532863@qq.comPurpose: Circular RNA (circRNA) plays an important role in various biological processes. However, their functions in cigarette smoke extract (CSE) induced human normal lung epithelial cells (BEAS-2B) injury remain vague. The study aimed to explore circRNA expression profiles and reveal their potential roles in CSE-treated BEAS-2B cells.Methods: 5% CSE exposure for 24 hours were used to build the BEAS-2B cells ferroptosis model. Differentially expressed circRNAs (DECs) were identified by next-generation RNA sequencing. Six randomly selected DECs were validated via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Ontology (GO) analysis were conducted to clarify the potential functions of the DECs. Furthermore, the role of hsa_circ_0025843 in CSE-related BEAS-2B cells ferroptosis was confirmed.Results: 5% CSE exposure induced BEAS-2B cells ferroptosis. Fifty-one up-regulated cirRNAs and 80 down-regulated circRNAs were revealed in CSE-treated BEAS-2B cells. Hsa_circ_0003461, hsa_circ_0007548, hsa_circ_0025843, hsa_circ_0068896, hsa_circ_0005832, and hsa_circ_0053378 were selected randomly to validate the reliability of next-generation RNA sequencing by qRT-PCR. After KEGG pathway analysis, DECs were found to participate in the process of EGFR tyrosine kinase inhibitor resistance and glycerophospholipid metabolism. The knockdown of hsa_circ_0025843 significantly alleviated CSE-induced BEAS-2B cells ferroptosis.Conclusion: The study indicated the circRNA expression profiles in CSE-treated BEAS-2B cells. Hsa_circ_0025843 alleviated CSE induced BEAS-2B cells ferroptosis, which might be a potential therapeutic target of CSE related lung injury.Keywords: cigarette smoke extract, Circular RNA, next-generation RNA sequencing, lung injury, ferroptosishttps://www.dovepress.com/circular-rna0025843-alleviated-cigarette-smoke-extract-induced-broncho-peer-reviewed-fulltext-article-COPDcigarette smoke extractcircular rnanext-generation rna sequencinglung injuryferroptosis |
| spellingShingle | Chen J Deng X Xie H Wang C Huang J Lian N Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells Ferroptosis International Journal of COPD cigarette smoke extract circular rna next-generation rna sequencing lung injury ferroptosis |
| title | Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells Ferroptosis |
| title_full | Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells Ferroptosis |
| title_fullStr | Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells Ferroptosis |
| title_full_unstemmed | Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells Ferroptosis |
| title_short | Circular RNA_0025843 Alleviated Cigarette Smoke Extract Induced Bronchoalveolar Epithelial Cells Ferroptosis |
| title_sort | circular rna 0025843 alleviated cigarette smoke extract induced bronchoalveolar epithelial cells ferroptosis |
| topic | cigarette smoke extract circular rna next-generation rna sequencing lung injury ferroptosis |
| url | https://www.dovepress.com/circular-rna0025843-alleviated-cigarette-smoke-extract-induced-broncho-peer-reviewed-fulltext-article-COPD |
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