Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast
Summary: Paxillin is a scaffold protein that participates in focal adhesion signaling in mammalian cells. Fission yeast paxillin ortholog, Pxl1, is required for contractile actomyosin ring (CAR) integrity and collaborates with the β-glucan synthase Bgs1 in septum formation. We show here that Pxl1’s...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-10-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124718315079 |
| _version_ | 1819263971489742848 |
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| author | Rebeca Martín-García Victor Arribas Pedro M. Coll Mario Pinar Raul A. Viana Sergio A. Rincón Jaime Correa-Bordes Juan Carlos Ribas Pilar Pérez |
| author_facet | Rebeca Martín-García Victor Arribas Pedro M. Coll Mario Pinar Raul A. Viana Sergio A. Rincón Jaime Correa-Bordes Juan Carlos Ribas Pilar Pérez |
| author_sort | Rebeca Martín-García |
| collection | DOAJ |
| description | Summary: Paxillin is a scaffold protein that participates in focal adhesion signaling in mammalian cells. Fission yeast paxillin ortholog, Pxl1, is required for contractile actomyosin ring (CAR) integrity and collaborates with the β-glucan synthase Bgs1 in septum formation. We show here that Pxl1’s main function is to recruit calcineurin (CN) phosphatase to the actomyosin ring; and thus the absence of either Pxl1 or calcineurin causes similar cytokinesis defects. In turn, CN participates in the dephosphorylation of the Cdc15 F-BAR protein, which recruits and concentrates Pxl1 at the CAR. Our findings suggest the existence of a positive feedback loop between Pxl1 and CN and establish that Pxl1 is a crucial component of the CN signaling pathway during cytokinesis. : Martín-García et al. show that fission yeast paxillin binds and recruits calcineurin (CN) phosphatase to the cytokinetic actomyosin ring (CAR). There, CN collaborates with Bgs1 in septum ingression and promotes the concentration of Pxl1 at the CAR in a feedback loop likely mediated through CN dephosphorylation of the Cdc15 F-BAR protein, essential for cytokinesis. Keywords: Schizosaccharomyces pombe, cytokinesis, calcineurin, paxillin, septum, actomyosin ring, Bgs1, Cdc15 |
| first_indexed | 2024-12-23T20:22:04Z |
| format | Article |
| id | doaj.art-e44fba83e4f34910b90e050daf1135d0 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-23T20:22:04Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e44fba83e4f34910b90e050daf1135d02022-12-21T17:32:31ZengElsevierCell Reports2211-12472018-10-01253772783.e4Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission YeastRebeca Martín-García0Victor Arribas1Pedro M. Coll2Mario Pinar3Raul A. Viana4Sergio A. Rincón5Jaime Correa-Bordes6Juan Carlos Ribas7Pilar Pérez8Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, SpainInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, SpainInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, SpainDepartamento de Biología Celular y Molecular, Centro de Investigaciones Biológicas del CSIC, Madrid 28040, SpainInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, SpainInstitut Curie, PSL Research University, CNRS, UMR 144, 75005 Paris, FranceDepartamento de Ciencias Biomédicas, Universidad de Extremadura, Badajoz, SpainInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, SpainInstituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain; Corresponding authorSummary: Paxillin is a scaffold protein that participates in focal adhesion signaling in mammalian cells. Fission yeast paxillin ortholog, Pxl1, is required for contractile actomyosin ring (CAR) integrity and collaborates with the β-glucan synthase Bgs1 in septum formation. We show here that Pxl1’s main function is to recruit calcineurin (CN) phosphatase to the actomyosin ring; and thus the absence of either Pxl1 or calcineurin causes similar cytokinesis defects. In turn, CN participates in the dephosphorylation of the Cdc15 F-BAR protein, which recruits and concentrates Pxl1 at the CAR. Our findings suggest the existence of a positive feedback loop between Pxl1 and CN and establish that Pxl1 is a crucial component of the CN signaling pathway during cytokinesis. : Martín-García et al. show that fission yeast paxillin binds and recruits calcineurin (CN) phosphatase to the cytokinetic actomyosin ring (CAR). There, CN collaborates with Bgs1 in septum ingression and promotes the concentration of Pxl1 at the CAR in a feedback loop likely mediated through CN dephosphorylation of the Cdc15 F-BAR protein, essential for cytokinesis. Keywords: Schizosaccharomyces pombe, cytokinesis, calcineurin, paxillin, septum, actomyosin ring, Bgs1, Cdc15http://www.sciencedirect.com/science/article/pii/S2211124718315079 |
| spellingShingle | Rebeca Martín-García Victor Arribas Pedro M. Coll Mario Pinar Raul A. Viana Sergio A. Rincón Jaime Correa-Bordes Juan Carlos Ribas Pilar Pérez Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast Cell Reports |
| title | Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast |
| title_full | Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast |
| title_fullStr | Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast |
| title_full_unstemmed | Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast |
| title_short | Paxillin-Mediated Recruitment of Calcineurin to the Contractile Ring Is Required for the Correct Progression of Cytokinesis in Fission Yeast |
| title_sort | paxillin mediated recruitment of calcineurin to the contractile ring is required for the correct progression of cytokinesis in fission yeast |
| url | http://www.sciencedirect.com/science/article/pii/S2211124718315079 |
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