Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review
Human adipose-derived stromal/stem cells (hASC) are widely used for in vitro modeling of physiologically relevant human adipose tissue. These models are useful for the development of tissue constructs for soft tissue regeneration and 3-dimensional (3D) microphysiological systems (MPS) for drug disco...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/6/1378 |
| _version_ | 1797531549812391936 |
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| author | Peyton Gibler Jeffrey Gimble Katie Hamel Emma Rogers Michael Henderson Xiying Wu Spencer Olesky Trivia Frazier |
| author_facet | Peyton Gibler Jeffrey Gimble Katie Hamel Emma Rogers Michael Henderson Xiying Wu Spencer Olesky Trivia Frazier |
| author_sort | Peyton Gibler |
| collection | DOAJ |
| description | Human adipose-derived stromal/stem cells (hASC) are widely used for in vitro modeling of physiologically relevant human adipose tissue. These models are useful for the development of tissue constructs for soft tissue regeneration and 3-dimensional (3D) microphysiological systems (MPS) for drug discovery. In this systematic review, we report on the current state of hASC culture and assessment methods for adipose tissue engineering using 3D MPS. Our search efforts resulted in the identification of 184 independent records, of which 27 were determined to be most relevant to the goals of the present review. Our results demonstrate a lack of consensus on methods for hASC culture and assessment for the production of physiologically relevant in vitro models of human adipose tissue. Few studies have assessed the impact of different 3D culture conditions on hASC adipogenesis. Additionally, there has been a limited use of assays for characterizing the functionality of adipose tissue in vitro. Results from this study suggest the need for more standardized culture methods and further analysis on in vitro tissue functionality. These will be necessary to validate the utility of 3D MPS as an in vitro model to reduce, refine, and replace in vivo experiments in the drug discovery regulatory process. |
| first_indexed | 2024-03-10T10:46:21Z |
| format | Article |
| id | doaj.art-e450849656ae42f5806c6d542ada031c |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T10:46:21Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-e450849656ae42f5806c6d542ada031c2023-11-21T22:37:08ZengMDPI AGCells2073-44092021-06-01106137810.3390/cells10061378Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic ReviewPeyton Gibler0Jeffrey Gimble1Katie Hamel2Emma Rogers3Michael Henderson4Xiying Wu5Spencer Olesky6Trivia Frazier7Obatala Sciences Inc., New Orleans, LA 70148, USAObatala Sciences Inc., New Orleans, LA 70148, USAObatala Sciences Inc., New Orleans, LA 70148, USAObatala Sciences Inc., New Orleans, LA 70148, USAObatala Sciences Inc., New Orleans, LA 70148, USAObatala Sciences Inc., New Orleans, LA 70148, USAObatala Sciences Inc., New Orleans, LA 70148, USAObatala Sciences Inc., New Orleans, LA 70148, USAHuman adipose-derived stromal/stem cells (hASC) are widely used for in vitro modeling of physiologically relevant human adipose tissue. These models are useful for the development of tissue constructs for soft tissue regeneration and 3-dimensional (3D) microphysiological systems (MPS) for drug discovery. In this systematic review, we report on the current state of hASC culture and assessment methods for adipose tissue engineering using 3D MPS. Our search efforts resulted in the identification of 184 independent records, of which 27 were determined to be most relevant to the goals of the present review. Our results demonstrate a lack of consensus on methods for hASC culture and assessment for the production of physiologically relevant in vitro models of human adipose tissue. Few studies have assessed the impact of different 3D culture conditions on hASC adipogenesis. Additionally, there has been a limited use of assays for characterizing the functionality of adipose tissue in vitro. Results from this study suggest the need for more standardized culture methods and further analysis on in vitro tissue functionality. These will be necessary to validate the utility of 3D MPS as an in vitro model to reduce, refine, and replace in vivo experiments in the drug discovery regulatory process.https://www.mdpi.com/2073-4409/10/6/1378adipose-derived stromal/stem cellsculture methods3-dimensionalmicrophysiological system |
| spellingShingle | Peyton Gibler Jeffrey Gimble Katie Hamel Emma Rogers Michael Henderson Xiying Wu Spencer Olesky Trivia Frazier Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review Cells adipose-derived stromal/stem cells culture methods 3-dimensional microphysiological system |
| title | Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review |
| title_full | Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review |
| title_fullStr | Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review |
| title_full_unstemmed | Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review |
| title_short | Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review |
| title_sort | human adipose derived stromal stem cell culture and analysis methods for adipose tissue modeling in vitro a systematic review |
| topic | adipose-derived stromal/stem cells culture methods 3-dimensional microphysiological system |
| url | https://www.mdpi.com/2073-4409/10/6/1378 |
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