Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo
Tian Zhou,1 Baiyan Sui,1 Xiumei Mo,2,* Jiao Sun1,* 1Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2017-05-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/multifunctional-and-biomimetic-fish-collagenbioactive-glass-nanofibers-peer-reviewed-article-IJN |
| _version_ | 1819021335357030400 |
|---|---|
| author | Zhou T Sui B Mo X Sun J |
| author_facet | Zhou T Sui B Mo X Sun J |
| author_sort | Zhou T |
| collection | DOAJ |
| description | Tian Zhou,1 Baiyan Sui,1 Xiumei Mo,2,* Jiao Sun1,* 1Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: The development of skin wound dressings with excellent properties has always been an important challenge in the field of biomedicine. In this study, biomimetic electrospun fish collagen/bioactive glass (Col/BG) nanofibers were prepared. Their structure, tensile strength, antibacterial activity and biological effects on human keratinocytes, human dermal fibroblasts and human vascular endothelial cells were investigated. Furthermore, the Sprague Dawley rat skin defect model was used to validate their effect on wound healing. The results showed that compared with pure fish collagen nanofibers, the tensile strength of the Col/BG nanofibers increased to 21.87±0.21 Mpa, with a certain degree of antibacterial activity against Staphylococcus aureus. It was also found that the Col/BG nanofibers promoted the adhesion, proliferation and migration of human keratinocytes. Col/BG nanofibers induced the secretion of type one collagen and vascular endothelial growth factor by human dermal fibroblasts, which further stimulated the proliferation of human vascular endothelial cells. Animal experimentation indicated that the Col/BG nanofibers could accelerate rat skin wound healing. This study developed a type of multifunctional and biomimetic fish Col/BG nanofibers, which had the ability to induce skin regeneration with adequate tensile strength and antibacterial activity. The Col/BG nanofibers are also easily available and inexpensive, providing the possibility for using as a functional skin wound dressing. Keywords: electrospun, collagen/bioactive glass nanofibers, antibacterial activity, skin regeneration, angiogenesis |
| first_indexed | 2024-12-21T04:05:28Z |
| format | Article |
| id | doaj.art-e4588d894c4448879bee95dc79795b8e |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-21T04:05:28Z |
| publishDate | 2017-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-e4588d894c4448879bee95dc79795b8e2022-12-21T19:16:37ZengDove Medical PressInternational Journal of Nanomedicine1178-20132017-05-01Volume 123495350732638Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivoZhou TSui BMo XSun JTian Zhou,1 Baiyan Sui,1 Xiumei Mo,2,* Jiao Sun1,* 1Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: The development of skin wound dressings with excellent properties has always been an important challenge in the field of biomedicine. In this study, biomimetic electrospun fish collagen/bioactive glass (Col/BG) nanofibers were prepared. Their structure, tensile strength, antibacterial activity and biological effects on human keratinocytes, human dermal fibroblasts and human vascular endothelial cells were investigated. Furthermore, the Sprague Dawley rat skin defect model was used to validate their effect on wound healing. The results showed that compared with pure fish collagen nanofibers, the tensile strength of the Col/BG nanofibers increased to 21.87±0.21 Mpa, with a certain degree of antibacterial activity against Staphylococcus aureus. It was also found that the Col/BG nanofibers promoted the adhesion, proliferation and migration of human keratinocytes. Col/BG nanofibers induced the secretion of type one collagen and vascular endothelial growth factor by human dermal fibroblasts, which further stimulated the proliferation of human vascular endothelial cells. Animal experimentation indicated that the Col/BG nanofibers could accelerate rat skin wound healing. This study developed a type of multifunctional and biomimetic fish Col/BG nanofibers, which had the ability to induce skin regeneration with adequate tensile strength and antibacterial activity. The Col/BG nanofibers are also easily available and inexpensive, providing the possibility for using as a functional skin wound dressing. Keywords: electrospun, collagen/bioactive glass nanofibers, antibacterial activity, skin regeneration, angiogenesishttps://www.dovepress.com/multifunctional-and-biomimetic-fish-collagenbioactive-glass-nanofibers-peer-reviewed-article-IJNelectrospuncollagen/bioactive glass nanofibersantibacterial activityskin regenerationangiogenesis |
| spellingShingle | Zhou T Sui B Mo X Sun J Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo International Journal of Nanomedicine electrospun collagen/bioactive glass nanofibers antibacterial activity skin regeneration angiogenesis |
| title | Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo |
| title_full | Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo |
| title_fullStr | Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo |
| title_full_unstemmed | Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo |
| title_short | Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo |
| title_sort | multifunctional and biomimetic fish collagen bioactive glass nanofibers fabrication antibacterial activity and inducing skin regeneration in vitro and in vivo |
| topic | electrospun collagen/bioactive glass nanofibers antibacterial activity skin regeneration angiogenesis |
| url | https://www.dovepress.com/multifunctional-and-biomimetic-fish-collagenbioactive-glass-nanofibers-peer-reviewed-article-IJN |
| work_keys_str_mv | AT zhout multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo AT suib multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo AT mox multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo AT sunj multifunctionalandbiomimeticfishcollagenbioactiveglassnanofibersfabricationantibacterialactivityandinducingskinregenerationinvitroandinvivo |