The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic Properties
Being superior to natural enzymes, nanoenzymes are drawing a great deal of attention in the field of biosensing. Herein, we developed an ultrasensitive, stable and selective colorimetric assay having dual functionalities of Au-tipped Pt nanorods (NRs). The optical and catalytic properties of Au-tipp...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/10/11/1285 |
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| author | Hao Gan Wenzhao Han Jiadi Liu Juntian Qi Hui Li Liping Wang |
| author_facet | Hao Gan Wenzhao Han Jiadi Liu Juntian Qi Hui Li Liping Wang |
| author_sort | Hao Gan |
| collection | DOAJ |
| description | Being superior to natural enzymes, nanoenzymes are drawing a great deal of attention in the field of biosensing. Herein, we developed an ultrasensitive, stable and selective colorimetric assay having dual functionalities of Au-tipped Pt nanorods (NRs). The optical and catalytic properties of Au-tipped Pt NRs were monitored using a spectrophotometer and the chromogenic substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the presence of H<sub>2</sub>O<sub>2</sub>, respectively. We found that Au-tipped Pt NRs exhibited excellent peroxidase-like activity, which decomposed hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) into oxygen (O<sub>2</sub>). The produced O<sub>2</sub> oxidized the chromogenic substrate into a blue color product. The oxidation rate of the chromogenic substrate could be monitored using a spectrophotometer at 652 nm. Notably, the peroxidase-like activity of Au-tipped Pt NRs decreased in the presence of ascorbic acid (AA). The produced O<sub>2</sub> preferentially reacted with AA, generating ascorbyl radicals (AA·) instead of oxidizing TMB, and thereby decreased the oxidation rate of TMB. Based on this inhibitory property, a selective colorimetric assay was developed using Au-tipped Pt NRs for the detection of AA. This work offers a novel detection method for AA. |
| first_indexed | 2024-03-10T15:05:34Z |
| format | Article |
| id | doaj.art-e45893658bd74cdc8249fdb4970ed5d8 |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T15:05:34Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Catalysts |
| spelling | doaj.art-e45893658bd74cdc8249fdb4970ed5d82023-11-20T19:52:34ZengMDPI AGCatalysts2073-43442020-11-011011128510.3390/catal10111285The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic PropertiesHao Gan0Wenzhao Han1Jiadi Liu2Juntian Qi3Hui Li4Liping Wang5Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, School of Life Sciences, Engineering Laboratory for AIDS Vaccine, Jilin Universtiy, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, Ministry of Education, School of Life Sciences, Engineering Laboratory for AIDS Vaccine, Jilin Universtiy, Changchun 130012, ChinaSchool of Plant Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, Ministry of Education, School of Life Sciences, Engineering Laboratory for AIDS Vaccine, Jilin Universtiy, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, Ministry of Education, School of Life Sciences, Engineering Laboratory for AIDS Vaccine, Jilin Universtiy, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, Ministry of Education, School of Life Sciences, Engineering Laboratory for AIDS Vaccine, Jilin Universtiy, Changchun 130012, ChinaBeing superior to natural enzymes, nanoenzymes are drawing a great deal of attention in the field of biosensing. Herein, we developed an ultrasensitive, stable and selective colorimetric assay having dual functionalities of Au-tipped Pt nanorods (NRs). The optical and catalytic properties of Au-tipped Pt NRs were monitored using a spectrophotometer and the chromogenic substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the presence of H<sub>2</sub>O<sub>2</sub>, respectively. We found that Au-tipped Pt NRs exhibited excellent peroxidase-like activity, which decomposed hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) into oxygen (O<sub>2</sub>). The produced O<sub>2</sub> oxidized the chromogenic substrate into a blue color product. The oxidation rate of the chromogenic substrate could be monitored using a spectrophotometer at 652 nm. Notably, the peroxidase-like activity of Au-tipped Pt NRs decreased in the presence of ascorbic acid (AA). The produced O<sub>2</sub> preferentially reacted with AA, generating ascorbyl radicals (AA·) instead of oxidizing TMB, and thereby decreased the oxidation rate of TMB. Based on this inhibitory property, a selective colorimetric assay was developed using Au-tipped Pt NRs for the detection of AA. This work offers a novel detection method for AA.https://www.mdpi.com/2073-4344/10/11/1285Au-tipped Ptnanoenzymeelectron transfercatalyticdetection |
| spellingShingle | Hao Gan Wenzhao Han Jiadi Liu Juntian Qi Hui Li Liping Wang The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic Properties Catalysts Au-tipped Pt nanoenzyme electron transfer catalytic detection |
| title | The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic Properties |
| title_full | The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic Properties |
| title_fullStr | The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic Properties |
| title_full_unstemmed | The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic Properties |
| title_short | The Mimic Enzyme Properties of Au@PtNRs and the Detection for Ascorbic Acid Based on Their Catalytic Properties |
| title_sort | mimic enzyme properties of au ptnrs and the detection for ascorbic acid based on their catalytic properties |
| topic | Au-tipped Pt nanoenzyme electron transfer catalytic detection |
| url | https://www.mdpi.com/2073-4344/10/11/1285 |
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