miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation.
This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3622609?pdf=render |
| _version_ | 1818188187095269376 |
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| author | Jose Luis Viñas Marina Ventayol Bernhard Brüne Michaela Jung Anna Sola Felip Pi Chrysoula Mastora Georgina Hotter |
| author_facet | Jose Luis Viñas Marina Ventayol Bernhard Brüne Michaela Jung Anna Sola Felip Pi Chrysoula Mastora Georgina Hotter |
| author_sort | Jose Luis Viñas |
| collection | DOAJ |
| description | This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production. |
| first_indexed | 2024-12-11T23:22:56Z |
| format | Article |
| id | doaj.art-e459687eea6e416bae434136b30e9b9c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T23:22:56Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e459687eea6e416bae434136b30e9b9c2022-12-22T00:46:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6093710.1371/journal.pone.0060937miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation.Jose Luis ViñasMarina VentayolBernhard BrüneMichaela JungAnna SolaFelip PiChrysoula MastoraGeorgina HotterThis study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.http://europepmc.org/articles/PMC3622609?pdf=render |
| spellingShingle | Jose Luis Viñas Marina Ventayol Bernhard Brüne Michaela Jung Anna Sola Felip Pi Chrysoula Mastora Georgina Hotter miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. PLoS ONE |
| title | miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. |
| title_full | miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. |
| title_fullStr | miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. |
| title_full_unstemmed | miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. |
| title_short | miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation. |
| title_sort | mirna let 7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation |
| url | http://europepmc.org/articles/PMC3622609?pdf=render |
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