Transcriptional response of Meloidogyne incognita to non-fumigant nematicides
Abstract There is limited research about the impacts of new nematicides, including fluazaindolizine, fluopyram, and fluensulfone, on the plant-parasitic nematode Meloidogyne incognita, despite it being a pervasive agricultural pest. In this study, M. incognita second-stage juveniles were exposed for...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13815-9 |
| _version_ | 1818236883358973952 |
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| author | Catherine L. Wram Cedar N. Hesse Inga A. Zasada |
| author_facet | Catherine L. Wram Cedar N. Hesse Inga A. Zasada |
| author_sort | Catherine L. Wram |
| collection | DOAJ |
| description | Abstract There is limited research about the impacts of new nematicides, including fluazaindolizine, fluopyram, and fluensulfone, on the plant-parasitic nematode Meloidogyne incognita, despite it being a pervasive agricultural pest. In this study, M. incognita second-stage juveniles were exposed for 24-h to fluensulfone, fluazaindolizine, fluopyram, and oxamyl and total RNA was extracted and sequenced using next-generation sequencing to determine gene expression. The effects of nematicide exposure on cellular detoxification pathways, common differentially expressed (DE) genes, and fatty acid and retinol-binding genes were examined. Fluopyram and oxamyl had the smallest impacts on the M. incognita transcriptome with 48 and 151 genes that were DE, respectively. These compounds also elicited a weak response in the cellular detoxification pathway and fatty acid and retinol-binding (FAR) genes. Fluensulfone and fluazaindolizine produced robust transcriptional responses with 1208 and 2611 DE genes, respectively. These compounds had strong impacts on cellular detoxification, causing differential regulation of transcription factors and genes in the detox pathway. These compounds strongly down-regulated FAR genes between 52–85%. Having a greater understanding of how these compounds function at a molecular level will help to promote proper stewardship, aid with nematicide discovery, and help to stay a step ahead of nematicide resistance. |
| first_indexed | 2024-12-12T12:16:56Z |
| format | Article |
| id | doaj.art-e459bd71a30a4708b68a211cd0516a91 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T12:16:56Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e459bd71a30a4708b68a211cd0516a912022-12-22T00:24:45ZengNature PortfolioScientific Reports2045-23222022-06-0112111210.1038/s41598-022-13815-9Transcriptional response of Meloidogyne incognita to non-fumigant nematicidesCatherine L. Wram0Cedar N. Hesse1Inga A. Zasada2Department of Botany and Plant Pathology, Oregon State UniversityUSDA-ARS Horticultural Crops Research UnitUSDA-ARS Horticultural Crops Research UnitAbstract There is limited research about the impacts of new nematicides, including fluazaindolizine, fluopyram, and fluensulfone, on the plant-parasitic nematode Meloidogyne incognita, despite it being a pervasive agricultural pest. In this study, M. incognita second-stage juveniles were exposed for 24-h to fluensulfone, fluazaindolizine, fluopyram, and oxamyl and total RNA was extracted and sequenced using next-generation sequencing to determine gene expression. The effects of nematicide exposure on cellular detoxification pathways, common differentially expressed (DE) genes, and fatty acid and retinol-binding genes were examined. Fluopyram and oxamyl had the smallest impacts on the M. incognita transcriptome with 48 and 151 genes that were DE, respectively. These compounds also elicited a weak response in the cellular detoxification pathway and fatty acid and retinol-binding (FAR) genes. Fluensulfone and fluazaindolizine produced robust transcriptional responses with 1208 and 2611 DE genes, respectively. These compounds had strong impacts on cellular detoxification, causing differential regulation of transcription factors and genes in the detox pathway. These compounds strongly down-regulated FAR genes between 52–85%. Having a greater understanding of how these compounds function at a molecular level will help to promote proper stewardship, aid with nematicide discovery, and help to stay a step ahead of nematicide resistance.https://doi.org/10.1038/s41598-022-13815-9 |
| spellingShingle | Catherine L. Wram Cedar N. Hesse Inga A. Zasada Transcriptional response of Meloidogyne incognita to non-fumigant nematicides Scientific Reports |
| title | Transcriptional response of Meloidogyne incognita to non-fumigant nematicides |
| title_full | Transcriptional response of Meloidogyne incognita to non-fumigant nematicides |
| title_fullStr | Transcriptional response of Meloidogyne incognita to non-fumigant nematicides |
| title_full_unstemmed | Transcriptional response of Meloidogyne incognita to non-fumigant nematicides |
| title_short | Transcriptional response of Meloidogyne incognita to non-fumigant nematicides |
| title_sort | transcriptional response of meloidogyne incognita to non fumigant nematicides |
| url | https://doi.org/10.1038/s41598-022-13815-9 |
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