Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil
Several studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no Leishmania specimens have been isolated from saliva. Here, we report the first isolation of Leishmania braziliensis from the saliva of humans with cuta...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2018-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000400401&lng=en&tlng=en |
| _version_ | 1797718476830277632 |
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| author | Maria Edileuza Felinto de Brito Ericka Lima Almeida Angela Cristina Rapela Medeiros Roberto Pereira Werkhäuser Joanna Lucia de Almeida Alexandre Bruna Santos Lima Figueiredo Sá Eduardo Henrique Gomes Rodrigues Sinval Pinto Brandão-Filho |
| author_facet | Maria Edileuza Felinto de Brito Ericka Lima Almeida Angela Cristina Rapela Medeiros Roberto Pereira Werkhäuser Joanna Lucia de Almeida Alexandre Bruna Santos Lima Figueiredo Sá Eduardo Henrique Gomes Rodrigues Sinval Pinto Brandão-Filho |
| author_sort | Maria Edileuza Felinto de Brito |
| collection | DOAJ |
| description | Several studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no Leishmania specimens have been isolated from saliva. Here, we report the first isolation of Leishmania braziliensis from the saliva of humans with cutaneous leishmaniasis but without lesions on their mucosa. The isolates were obtained from salivary fluid inoculated in hamsters and were tested by multilocus enzyme electrophoresis. Seven samples from 43 patients suspected of having the disease were identified for in vivo culture. These findings suggest that saliva is a clinical sample that allows the isolation of Leishmania sp. |
| first_indexed | 2024-03-12T08:51:22Z |
| format | Article |
| id | doaj.art-e45a11eca0bb41b1a89afb2ba11bab9c |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:51:22Z |
| publishDate | 2018-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-e45a11eca0bb41b1a89afb2ba11bab9c2023-09-02T16:17:54ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602018-02-01113410.1590/0074-02760170250S0074-02762018000400401Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern BrazilMaria Edileuza Felinto de BritoEricka Lima AlmeidaAngela Cristina Rapela MedeirosRoberto Pereira WerkhäuserJoanna Lucia de Almeida AlexandreBruna Santos Lima Figueiredo SáEduardo Henrique Gomes RodriguesSinval Pinto Brandão-FilhoSeveral studies have described the use of non-invasive collection methods, mostly based on the detection of parasite DNA, for diagnosis. However, no Leishmania specimens have been isolated from saliva. Here, we report the first isolation of Leishmania braziliensis from the saliva of humans with cutaneous leishmaniasis but without lesions on their mucosa. The isolates were obtained from salivary fluid inoculated in hamsters and were tested by multilocus enzyme electrophoresis. Seven samples from 43 patients suspected of having the disease were identified for in vivo culture. These findings suggest that saliva is a clinical sample that allows the isolation of Leishmania sp.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000400401&lng=en&tlng=enLeishmania braziliensissalivaculture media |
| spellingShingle | Maria Edileuza Felinto de Brito Ericka Lima Almeida Angela Cristina Rapela Medeiros Roberto Pereira Werkhäuser Joanna Lucia de Almeida Alexandre Bruna Santos Lima Figueiredo Sá Eduardo Henrique Gomes Rodrigues Sinval Pinto Brandão-Filho Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil Memorias do Instituto Oswaldo Cruz Leishmania braziliensis saliva culture media |
| title | Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil |
| title_full | Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil |
| title_fullStr | Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil |
| title_full_unstemmed | Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil |
| title_short | Leishmania (Viannia) braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis-endemic area of northeastern Brazil |
| title_sort | leishmania viannia braziliensis isolated from the saliva of patients in a cutaneous leishmaniasis endemic area of northeastern brazil |
| topic | Leishmania braziliensis saliva culture media |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000400401&lng=en&tlng=en |
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