Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats
Vascular dementia is a transversal phenomenon in different kinds of neurodegenerative diseases involving acute and chronic brain alterations. Specifically, the role of phospholipids in the pathogenesis of dementia remains unknown. In the present study, we explored phospholipid profiles a month posti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnins.2018.00989/full |
| _version_ | 1811277476342530048 |
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| author | Angelica Maria Sabogal-Guáqueta Javier Gustavo Villamil-Ortiz Julian David Arias-Londoño Gloria Patricia Cardona-Gómez |
| author_facet | Angelica Maria Sabogal-Guáqueta Javier Gustavo Villamil-Ortiz Julian David Arias-Londoño Gloria Patricia Cardona-Gómez |
| author_sort | Angelica Maria Sabogal-Guáqueta |
| collection | DOAJ |
| description | Vascular dementia is a transversal phenomenon in different kinds of neurodegenerative diseases involving acute and chronic brain alterations. Specifically, the role of phospholipids in the pathogenesis of dementia remains unknown. In the present study, we explored phospholipid profiles a month postischemia in cognitively impaired rats. The two-vessel occlusion (2-VO) model was used to generate brain parenchyma ischemia in adult male rats confirmed by alterations in myelin, endothelium, astrocytes and inflammation mediator. A lipidomic analysis was performed via mass spectrometry in the hippocampus and serum a month postischemia. We found decreases in phospholipids (PLs) associated with neurotransmission, such as phosphatidylcholine (PC 32:0, PC 34:2, PC 36:3, PC 36:4, and PC 42:1), and increases in PLs implied in membrane structure and signaling, such as lysophosphatidylethanolamine (LPE 18:1, 20:3, and 22:6) and phosphatidylserine (PS 38:4, 36:2, and 40:4), in the hippocampus. Complementarily, PC (PC 34:2, PC 34:3, PC 38:5, and PC 36:5) and ether-PC (ePC 34:1, 34:2, 36:2, 38:2, and 38:3) decreased, while Lyso-PC (LPC 18:0, 18:1, 20:4, 20:5, and LPC 22:6) and phosphatidylinositol (PI 36:2, 38:4, 38:5, and 40:5), as neurovascular state sensors, increased in the serum. Taken together, these data suggest inverse PC/LPC-PI levels as peripheral biomarkers and inverse PC/LPE-PS as a central indicator of postischemic cognitive impairment in rats. |
| first_indexed | 2024-04-13T00:17:23Z |
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| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-13T00:17:23Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-e45e6cb3d8f04b3d9807f3a3d02dcbc82022-12-22T03:10:54ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2018-12-011210.3389/fnins.2018.00989413311Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in RatsAngelica Maria Sabogal-Guáqueta0Javier Gustavo Villamil-Ortiz1Julian David Arias-Londoño2Gloria Patricia Cardona-Gómez3Neuroscience Group of Antioquia, Cellular and Molecular Neurobiology Area – School of Medicine, Sede de Investigación Universitaria (SIU), University of Antioquia, Medellin, ColombiaNeuroscience Group of Antioquia, Cellular and Molecular Neurobiology Area – School of Medicine, Sede de Investigación Universitaria (SIU), University of Antioquia, Medellin, ColombiaDepartment of Systems Engineering, University of Antioquia, Medellín, ColombiaNeuroscience Group of Antioquia, Cellular and Molecular Neurobiology Area – School of Medicine, Sede de Investigación Universitaria (SIU), University of Antioquia, Medellin, ColombiaVascular dementia is a transversal phenomenon in different kinds of neurodegenerative diseases involving acute and chronic brain alterations. Specifically, the role of phospholipids in the pathogenesis of dementia remains unknown. In the present study, we explored phospholipid profiles a month postischemia in cognitively impaired rats. The two-vessel occlusion (2-VO) model was used to generate brain parenchyma ischemia in adult male rats confirmed by alterations in myelin, endothelium, astrocytes and inflammation mediator. A lipidomic analysis was performed via mass spectrometry in the hippocampus and serum a month postischemia. We found decreases in phospholipids (PLs) associated with neurotransmission, such as phosphatidylcholine (PC 32:0, PC 34:2, PC 36:3, PC 36:4, and PC 42:1), and increases in PLs implied in membrane structure and signaling, such as lysophosphatidylethanolamine (LPE 18:1, 20:3, and 22:6) and phosphatidylserine (PS 38:4, 36:2, and 40:4), in the hippocampus. Complementarily, PC (PC 34:2, PC 34:3, PC 38:5, and PC 36:5) and ether-PC (ePC 34:1, 34:2, 36:2, 38:2, and 38:3) decreased, while Lyso-PC (LPC 18:0, 18:1, 20:4, 20:5, and LPC 22:6) and phosphatidylinositol (PI 36:2, 38:4, 38:5, and 40:5), as neurovascular state sensors, increased in the serum. Taken together, these data suggest inverse PC/LPC-PI levels as peripheral biomarkers and inverse PC/LPE-PS as a central indicator of postischemic cognitive impairment in rats.https://www.frontiersin.org/article/10.3389/fnins.2018.00989/fullglobal ischemiacognitive impairmentphospholipid profilebiomarkersserumhippocampus |
| spellingShingle | Angelica Maria Sabogal-Guáqueta Javier Gustavo Villamil-Ortiz Julian David Arias-Londoño Gloria Patricia Cardona-Gómez Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats Frontiers in Neuroscience global ischemia cognitive impairment phospholipid profile biomarkers serum hippocampus |
| title | Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats |
| title_full | Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats |
| title_fullStr | Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats |
| title_full_unstemmed | Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats |
| title_short | Inverse Phosphatidylcholine/Phosphatidylinositol Levels as Peripheral Biomarkers and Phosphatidylcholine/Lysophosphatidylethanolamine-Phosphatidylserine as Hippocampal Indicator of Postischemic Cognitive Impairment in Rats |
| title_sort | inverse phosphatidylcholine phosphatidylinositol levels as peripheral biomarkers and phosphatidylcholine lysophosphatidylethanolamine phosphatidylserine as hippocampal indicator of postischemic cognitive impairment in rats |
| topic | global ischemia cognitive impairment phospholipid profile biomarkers serum hippocampus |
| url | https://www.frontiersin.org/article/10.3389/fnins.2018.00989/full |
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