Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic Evaluation
Among the fungal diseases of apple trees, serious yield losses are due to an apple scab caused by <i>Venturia inaequalis</i>. Protection against this disease is based mainly on chemical treatments, which are currently very limited. Therefore, it is extremely important to introduce cultiv...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/527 |
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| author | Małgorzata Podwyszyńska Monika Markiewicz Agata Broniarek-Niemiec Bożena Matysiak Agnieszka Marasek-Ciolakowska |
| author_facet | Małgorzata Podwyszyńska Monika Markiewicz Agata Broniarek-Niemiec Bożena Matysiak Agnieszka Marasek-Ciolakowska |
| author_sort | Małgorzata Podwyszyńska |
| collection | DOAJ |
| description | Among the fungal diseases of apple trees, serious yield losses are due to an apple scab caused by <i>Venturia inaequalis</i>. Protection against this disease is based mainly on chemical treatments, which are currently very limited. Therefore, it is extremely important to introduce cultivars with reduced susceptibility to this pathogen. One of the important sources of variability for breeding is the process of polyploidization. Newly obtained polyploids may acquire new features, including increased resistance to diseases. In our earlier studies, numerous tetraploids have been obtained for several apple cultivars with ‘Free Redstar’ tetraploids manifesting enhanced resistance to apple scab. In the present study, tetraploids of ‘Free Redstar’ were assessed in terms of phenotype and genotype with particular emphasis on the genetic background of their increased resistance to apple scab. Compared to diploid plants, tetraploids (own-rooted plants) were characterized with poor growth, especially during first growing season. They had considerably shorter shoots, fewer branches, smaller stem diameter, and reshaped leaves. In contrast to own-rooted plants, in M9-grafted three-year old trees, no significant differences between diplo- and tetraploids were observed, either in morphological or physiological parameters, with the exceptions of the increased leaf thickness and chlorophyll content recorded in tetraploids. Significant differences between sibling tetraploid clones were recorded, particularly in leaf shape and some physiological parameters. The amplified fragment length polymorphism (AFLP) analysis confirmed genetic polymorphism of tetraploid clones. Methylation-sensitive amplification polymorphism (MSAP) analysis showed that the level of DNA methylation was twice as high in young tetraploid plants as in a diploid donor tree, which may explain the weaker vigour of neotetraploids in the early period of their growth in the juvenile phase. Molecular analysis showed that ‘Free Redstar’ cultivar and their tetraploids bear six <i>Rvi</i> genes (<i>Rvi5</i>, <i>Rvi6</i>, <i>Rvi8</i>, <i>Rvi11</i>, <i>Rvi14</i> and <i>Rvi17</i>). Transcriptome analysis confirmed enhanced resistance to apple scab of ‘Free Redstar’ tetraploids since the expression levels of genes related to resistance were strongly enhanced in tetraploids compared to their diploid counterparts. |
| first_indexed | 2024-03-09T05:49:42Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
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| last_indexed | 2024-03-09T05:49:42Z |
| publishDate | 2021-01-01 |
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| spelling | doaj.art-e45e7c68944845b0a63731c5f56a55502023-12-03T12:18:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122252710.3390/ijms22020527Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic EvaluationMałgorzata Podwyszyńska0Monika Markiewicz1Agata Broniarek-Niemiec2Bożena Matysiak3Agnieszka Marasek-Ciolakowska4Department of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Street, 96-100 Skierniewice, PolandDepartment of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Street, 96-100 Skierniewice, PolandDepartment of Phytopathology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Street, 96-100 Skierniewice, PolandDepartment of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Street, 96-100 Skierniewice, PolandDepartment of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Street, 96-100 Skierniewice, PolandAmong the fungal diseases of apple trees, serious yield losses are due to an apple scab caused by <i>Venturia inaequalis</i>. Protection against this disease is based mainly on chemical treatments, which are currently very limited. Therefore, it is extremely important to introduce cultivars with reduced susceptibility to this pathogen. One of the important sources of variability for breeding is the process of polyploidization. Newly obtained polyploids may acquire new features, including increased resistance to diseases. In our earlier studies, numerous tetraploids have been obtained for several apple cultivars with ‘Free Redstar’ tetraploids manifesting enhanced resistance to apple scab. In the present study, tetraploids of ‘Free Redstar’ were assessed in terms of phenotype and genotype with particular emphasis on the genetic background of their increased resistance to apple scab. Compared to diploid plants, tetraploids (own-rooted plants) were characterized with poor growth, especially during first growing season. They had considerably shorter shoots, fewer branches, smaller stem diameter, and reshaped leaves. In contrast to own-rooted plants, in M9-grafted three-year old trees, no significant differences between diplo- and tetraploids were observed, either in morphological or physiological parameters, with the exceptions of the increased leaf thickness and chlorophyll content recorded in tetraploids. Significant differences between sibling tetraploid clones were recorded, particularly in leaf shape and some physiological parameters. The amplified fragment length polymorphism (AFLP) analysis confirmed genetic polymorphism of tetraploid clones. Methylation-sensitive amplification polymorphism (MSAP) analysis showed that the level of DNA methylation was twice as high in young tetraploid plants as in a diploid donor tree, which may explain the weaker vigour of neotetraploids in the early period of their growth in the juvenile phase. Molecular analysis showed that ‘Free Redstar’ cultivar and their tetraploids bear six <i>Rvi</i> genes (<i>Rvi5</i>, <i>Rvi6</i>, <i>Rvi8</i>, <i>Rvi11</i>, <i>Rvi14</i> and <i>Rvi17</i>). Transcriptome analysis confirmed enhanced resistance to apple scab of ‘Free Redstar’ tetraploids since the expression levels of genes related to resistance were strongly enhanced in tetraploids compared to their diploid counterparts.https://www.mdpi.com/1422-0067/22/2/527polyploidizationDNA methylationdisease-resistance related genes<i>Rvi</i> genes<i>Venturia inaequalis</i><i>Malus</i> × <i>domestica</i> |
| spellingShingle | Małgorzata Podwyszyńska Monika Markiewicz Agata Broniarek-Niemiec Bożena Matysiak Agnieszka Marasek-Ciolakowska Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic Evaluation International Journal of Molecular Sciences polyploidization DNA methylation disease-resistance related genes <i>Rvi</i> genes <i>Venturia inaequalis</i> <i>Malus</i> × <i>domestica</i> |
| title | Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic Evaluation |
| title_full | Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic Evaluation |
| title_fullStr | Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic Evaluation |
| title_full_unstemmed | Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic Evaluation |
| title_short | Apple Autotetraploids with Enhanced Resistance to Apple Scab (<i>Venturia inaequalis</i>) Due to Genome Duplication-Phenotypic and Genetic Evaluation |
| title_sort | apple autotetraploids with enhanced resistance to apple scab i venturia inaequalis i due to genome duplication phenotypic and genetic evaluation |
| topic | polyploidization DNA methylation disease-resistance related genes <i>Rvi</i> genes <i>Venturia inaequalis</i> <i>Malus</i> × <i>domestica</i> |
| url | https://www.mdpi.com/1422-0067/22/2/527 |
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