Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts
Bacterial ghosts (BGs) are promising vaccine platforms owing to their high adjuvant properties and delivery efficiency. Heterologous antigens can be anchored to different parts of BGs using genetic engineering strategies to prepare vaccines. However, several key issues need to be resolved, including...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1349746/full |
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| author | Huaiyu Zhang Huaiyu Zhang Zhaocai Li Wei Li Youshun Jin Yunhui Li Qian Xiao Dewen Tong Jizhang Zhou Jizhang Zhou |
| author_facet | Huaiyu Zhang Huaiyu Zhang Zhaocai Li Wei Li Youshun Jin Yunhui Li Qian Xiao Dewen Tong Jizhang Zhou Jizhang Zhou |
| author_sort | Huaiyu Zhang |
| collection | DOAJ |
| description | Bacterial ghosts (BGs) are promising vaccine platforms owing to their high adjuvant properties and delivery efficiency. Heterologous antigens can be anchored to different parts of BGs using genetic engineering strategies to prepare vaccines. However, several key issues need to be resolved, including the efficient preparation of BGs and determining the optimal anchoring position of exogenous antigens in the BGs. Here, we prepared an efficient temperature-controlled lysis system using lysis gene E of phage PhiX174 and used the major outer membrane protein (MOMP) of Chlamydia abortus (C. abortus) as a model antigen to explore the optimal display location of exogenous antigens in BGs. We demonstrated that the constructed recombinant temperature-controlled lysis plasmid can still stably inhibit E gene expression at 37°C, and the lysis efficiency of E. coli can reach above 99.9%. Four recombinant MOMP Escherichia coli (E. coli) ghost vaccines were constructed using different anchor sequences. These vaccines all induced strong specific antibody responses and secrete high levels of IFN-γ in immunized mice and significantly increased the clearance of C. abortus in a mouse infection model. Notably, the strongest immune effect was observed when MOMP was displayed on the surface of E. coli ghosts (rECG-InpN-M), which resulted in the clearance of C. abortus in mice 6 days earlier than that with the recombinant MOMP vaccine. Altogether, we constructed an efficient BG temperature-controlled lysis system and provided a feasible strategy for developing a BG delivery platform with enhanced immune effects. |
| first_indexed | 2024-03-08T04:11:00Z |
| format | Article |
| id | doaj.art-e45f91b119614458bbe5de100d2943b8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T04:11:00Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e45f91b119614458bbe5de100d2943b82024-02-08T19:43:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13497461349746Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghostsHuaiyu Zhang0Huaiyu Zhang1Zhaocai Li2Wei Li3Youshun Jin4Yunhui Li5Qian Xiao6Dewen Tong7Jizhang Zhou8Jizhang Zhou9State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaGansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou, ChinaBacterial ghosts (BGs) are promising vaccine platforms owing to their high adjuvant properties and delivery efficiency. Heterologous antigens can be anchored to different parts of BGs using genetic engineering strategies to prepare vaccines. However, several key issues need to be resolved, including the efficient preparation of BGs and determining the optimal anchoring position of exogenous antigens in the BGs. Here, we prepared an efficient temperature-controlled lysis system using lysis gene E of phage PhiX174 and used the major outer membrane protein (MOMP) of Chlamydia abortus (C. abortus) as a model antigen to explore the optimal display location of exogenous antigens in BGs. We demonstrated that the constructed recombinant temperature-controlled lysis plasmid can still stably inhibit E gene expression at 37°C, and the lysis efficiency of E. coli can reach above 99.9%. Four recombinant MOMP Escherichia coli (E. coli) ghost vaccines were constructed using different anchor sequences. These vaccines all induced strong specific antibody responses and secrete high levels of IFN-γ in immunized mice and significantly increased the clearance of C. abortus in a mouse infection model. Notably, the strongest immune effect was observed when MOMP was displayed on the surface of E. coli ghosts (rECG-InpN-M), which resulted in the clearance of C. abortus in mice 6 days earlier than that with the recombinant MOMP vaccine. Altogether, we constructed an efficient BG temperature-controlled lysis system and provided a feasible strategy for developing a BG delivery platform with enhanced immune effects.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1349746/fullbacterial ghostsvaccinedelivery vectorChlamydia abortusMOMP antigen |
| spellingShingle | Huaiyu Zhang Huaiyu Zhang Zhaocai Li Wei Li Youshun Jin Yunhui Li Qian Xiao Dewen Tong Jizhang Zhou Jizhang Zhou Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts Frontiers in Microbiology bacterial ghosts vaccine delivery vector Chlamydia abortus MOMP antigen |
| title | Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts |
| title_full | Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts |
| title_fullStr | Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts |
| title_full_unstemmed | Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts |
| title_short | Comparison of the immune effects of the Chlamydia abortus MOMP antigen displayed in different parts of bacterial ghosts |
| title_sort | comparison of the immune effects of the chlamydia abortus momp antigen displayed in different parts of bacterial ghosts |
| topic | bacterial ghosts vaccine delivery vector Chlamydia abortus MOMP antigen |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1349746/full |
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