Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel Disease
We explored the fecal microbiota in pediatric patients <18 years of age with treatment-naïve IBD (80 Crohn’s disease (CD), 27 ulcerative colitis (UC)), in 50 non-IBD patients with gastrointestinal symptoms without inflammation and in 75 healthy children. Using a targeted qPCR approach, the quanti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1273 |
| _version_ | 1797433355673796608 |
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| author | Christine Olbjørn Milada Cvancarova Småstuen Aina Elisabeth Fossum Moen |
| author_facet | Christine Olbjørn Milada Cvancarova Småstuen Aina Elisabeth Fossum Moen |
| author_sort | Christine Olbjørn |
| collection | DOAJ |
| description | We explored the fecal microbiota in pediatric patients <18 years of age with treatment-naïve IBD (80 Crohn’s disease (CD), 27 ulcerative colitis (UC)), in 50 non-IBD patients with gastrointestinal symptoms without inflammation and in 75 healthy children. Using a targeted qPCR approach, the quantities of more than 100 different bacterial species were measured. <b>Results:</b> The bacterial abundance was statistically significantly reduced in the IBD and non-IBD patients compared to the healthy children for several beneficial species. The CD patients had a lower abundance of <i>Bifidobacterium</i> species compared to the UC patients, and the IBD patients in need of biologic therapy had a lower abundance of butyrate producing bacteria. Based on the abundance of bacterial species at diagnosis, we constructed Diagnostic, Phenotype and Prognostic Indexes. Patients with a high Diagnostic Index had 2.5 times higher odds for having IBD than those with a lower index. The CD patients had a higher Phenotype Index than the UC patients. Patients with a high Prognostic Index had 2.1 higher odds for needing biologic therapy compared to those with a lower index. <b>Conclusions:</b> The fecal abundance of bacterial species can aid in diagnosing IBD, in distinguishing CD from UC and in identifying children with IBD in need of biologic therapy. |
| first_indexed | 2024-03-09T10:14:51Z |
| format | Article |
| id | doaj.art-e460ac3e6d6447698e0c530d68ffaf34 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:14:51Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e460ac3e6d6447698e0c530d68ffaf342023-12-01T22:28:02ZengMDPI AGMicroorganisms2076-26072022-06-01107127310.3390/microorganisms10071273Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel DiseaseChristine Olbjørn0Milada Cvancarova Småstuen1Aina Elisabeth Fossum Moen2Department for Paediatric and Adolescent Medicine, Akershus University Hospital, NO-1470 Lørenskog, NorwayDepartment of Nursing and Health Promotion, Oslo Metropolitan University, NO-0176 Oslo, NorwayDivision of Infection Control and Environmental Health, Norwegian Institute of Public Health, NO-0213 Oslo, NorwayWe explored the fecal microbiota in pediatric patients <18 years of age with treatment-naïve IBD (80 Crohn’s disease (CD), 27 ulcerative colitis (UC)), in 50 non-IBD patients with gastrointestinal symptoms without inflammation and in 75 healthy children. Using a targeted qPCR approach, the quantities of more than 100 different bacterial species were measured. <b>Results:</b> The bacterial abundance was statistically significantly reduced in the IBD and non-IBD patients compared to the healthy children for several beneficial species. The CD patients had a lower abundance of <i>Bifidobacterium</i> species compared to the UC patients, and the IBD patients in need of biologic therapy had a lower abundance of butyrate producing bacteria. Based on the abundance of bacterial species at diagnosis, we constructed Diagnostic, Phenotype and Prognostic Indexes. Patients with a high Diagnostic Index had 2.5 times higher odds for having IBD than those with a lower index. The CD patients had a higher Phenotype Index than the UC patients. Patients with a high Prognostic Index had 2.1 higher odds for needing biologic therapy compared to those with a lower index. <b>Conclusions:</b> The fecal abundance of bacterial species can aid in diagnosing IBD, in distinguishing CD from UC and in identifying children with IBD in need of biologic therapy.https://www.mdpi.com/2076-2607/10/7/1273IBDmicrobiotapediatricprognosisbiologic therapybiomarker |
| spellingShingle | Christine Olbjørn Milada Cvancarova Småstuen Aina Elisabeth Fossum Moen Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel Disease Microorganisms IBD microbiota pediatric prognosis biologic therapy biomarker |
| title | Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel Disease |
| title_full | Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel Disease |
| title_fullStr | Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel Disease |
| title_full_unstemmed | Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel Disease |
| title_short | Targeted Analysis of the Gut Microbiome for Diagnosis, Prognosis and Treatment Individualization in Pediatric Inflammatory Bowel Disease |
| title_sort | targeted analysis of the gut microbiome for diagnosis prognosis and treatment individualization in pediatric inflammatory bowel disease |
| topic | IBD microbiota pediatric prognosis biologic therapy biomarker |
| url | https://www.mdpi.com/2076-2607/10/7/1273 |
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