Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan
Thalamita danae (Stimpson1858), re-described as a new report, based on an integrative taxonomy approach combining 16S rRNA partial sequence of mitochondrial DNA and morphological analyses was used for the accurate identification of specimens. The morphological and molecular analysis provides the con...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2018.1438855 |
| _version_ | 1827774325260812288 |
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| author | Noor Us Saher Farah Naz Syeda Mariam Siddiqa Mohammad Mumtaz Khan Mustafa Kamal |
| author_facet | Noor Us Saher Farah Naz Syeda Mariam Siddiqa Mohammad Mumtaz Khan Mustafa Kamal |
| author_sort | Noor Us Saher |
| collection | DOAJ |
| description | Thalamita danae (Stimpson1858), re-described as a new report, based on an integrative taxonomy approach combining 16S rRNA partial sequence of mitochondrial DNA and morphological analyses was used for the accurate identification of specimens. The morphological and molecular analysis provides the confirm evidence of T. danae in coastal waters of Pakistan. Results were confirmed by amplification of partial sequences of 16S rRNA mt-DNA gene and the sequence was searched for similarity using BLASTn (Basic Local Alignment Tool), the result showed 97% sequence similarity with partial sequences of T. danae Stimpson, 1858 as obtained from gene bank. The attained sequence was submitted to gene bank after confirmation of genetic and morphological similarity. High sequence similarity with accession no: FJ152165.1 indicated, that misidentification of species does not occur. |
| first_indexed | 2024-03-11T13:35:51Z |
| format | Article |
| id | doaj.art-e46419a2e2294506bd0beac6268395a6 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:35:51Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-e46419a2e2294506bd0beac6268395a62023-11-02T15:57:22ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592018-01-013133233510.1080/23802359.2018.14388551438855Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, PakistanNoor Us Saher0Farah Naz1Syeda Mariam Siddiqa2Mohammad Mumtaz Khan3Mustafa Kamal4University of KarachiUniversity of KarachiUniversity of KarachiUniversity of HaripurUniversity of KarachiThalamita danae (Stimpson1858), re-described as a new report, based on an integrative taxonomy approach combining 16S rRNA partial sequence of mitochondrial DNA and morphological analyses was used for the accurate identification of specimens. The morphological and molecular analysis provides the confirm evidence of T. danae in coastal waters of Pakistan. Results were confirmed by amplification of partial sequences of 16S rRNA mt-DNA gene and the sequence was searched for similarity using BLASTn (Basic Local Alignment Tool), the result showed 97% sequence similarity with partial sequences of T. danae Stimpson, 1858 as obtained from gene bank. The attained sequence was submitted to gene bank after confirmation of genetic and morphological similarity. High sequence similarity with accession no: FJ152165.1 indicated, that misidentification of species does not occur.http://dx.doi.org/10.1080/23802359.2018.1438855brachyurasystematicsmolecular phylogenymt dnapakistan coast |
| spellingShingle | Noor Us Saher Farah Naz Syeda Mariam Siddiqa Mohammad Mumtaz Khan Mustafa Kamal Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan Mitochondrial DNA. Part B. Resources brachyura systematics molecular phylogeny mt dna pakistan coast |
| title | Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan |
| title_full | Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan |
| title_fullStr | Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan |
| title_full_unstemmed | Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan |
| title_short | Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan |
| title_sort | re description and molecular identification of thalamita danae stimpson 1858 decapoda brachyura portunidae based on fresh material from the coastal waters pakistan |
| topic | brachyura systematics molecular phylogeny mt dna pakistan coast |
| url | http://dx.doi.org/10.1080/23802359.2018.1438855 |
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