Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>
Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-reso...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/11/1774 |
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| author | Xian Ju Xingxing Fang Yunzhu Xiao Bingyu Li Ruoping Shi Chaoliang Wei Conghui You |
| author_facet | Xian Ju Xingxing Fang Yunzhu Xiao Bingyu Li Ruoping Shi Chaoliang Wei Conghui You |
| author_sort | Xian Ju |
| collection | DOAJ |
| description | Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-resolution RNA-seq assay, we compared the transcriptomes of a wild-type <i>Escherichia coli</i> K-12 strain and its <i>gcvB</i> deletion derivative grown in minimal medium and identified putative targets responding to GcvB, including <i>flu</i>, a determinant gene of auto-aggregation. The following molecular studies and the enhanced auto-aggregation ability of the <i>gcvB</i> knockout strain further substantiated the induced expression of these genes. Intriguingly, the reduced expression of OxyR (the oxidative stress regulator) in the <i>gcvB</i> knockout strain was identified to account for the increased expression of <i>flu</i>. Additionally, GcvB was characterized to up-regulate the expression of OxyR at the translational level. Accordingly, compared to the wild type, the GcvB deletion strain was more sensitive to oxidative stress and lost some its ability to eliminate endogenous reactive oxygen species. Taken together, we reveal that GcvB regulates oxidative stress response by up-regulating OxyR expression. Our findings provide an insight into the diversity of GcvB regulation and add an additional layer to the regulation of OxyR. |
| first_indexed | 2024-03-10T05:45:39Z |
| format | Article |
| id | doaj.art-e4654f4491fe4dd3b94510491b93957b |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T05:45:39Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-e4654f4491fe4dd3b94510491b93957b2023-11-22T22:13:30ZengMDPI AGAntioxidants2076-39212021-11-011011177410.3390/antiox10111774Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i>Xian Ju0Xingxing Fang1Yunzhu Xiao2Bingyu Li3Ruoping Shi4Chaoliang Wei5Conghui You6Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanology, Shenzhen University, Shenzhen 518055, ChinaShenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanology, Shenzhen University, Shenzhen 518055, ChinaShenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanology, Shenzhen University, Shenzhen 518055, ChinaShenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanology, Shenzhen University, Shenzhen 518055, ChinaShenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanology, Shenzhen University, Shenzhen 518055, ChinaHealth Science Center, Shenzhen University, Shenzhen 518055, ChinaShenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanology, Shenzhen University, Shenzhen 518055, ChinaSmall non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-resolution RNA-seq assay, we compared the transcriptomes of a wild-type <i>Escherichia coli</i> K-12 strain and its <i>gcvB</i> deletion derivative grown in minimal medium and identified putative targets responding to GcvB, including <i>flu</i>, a determinant gene of auto-aggregation. The following molecular studies and the enhanced auto-aggregation ability of the <i>gcvB</i> knockout strain further substantiated the induced expression of these genes. Intriguingly, the reduced expression of OxyR (the oxidative stress regulator) in the <i>gcvB</i> knockout strain was identified to account for the increased expression of <i>flu</i>. Additionally, GcvB was characterized to up-regulate the expression of OxyR at the translational level. Accordingly, compared to the wild type, the GcvB deletion strain was more sensitive to oxidative stress and lost some its ability to eliminate endogenous reactive oxygen species. Taken together, we reveal that GcvB regulates oxidative stress response by up-regulating OxyR expression. Our findings provide an insight into the diversity of GcvB regulation and add an additional layer to the regulation of OxyR.https://www.mdpi.com/2076-3921/10/11/1774<i>Escherichia coli</i>small RNAGcvBoxidative stressOxyR |
| spellingShingle | Xian Ju Xingxing Fang Yunzhu Xiao Bingyu Li Ruoping Shi Chaoliang Wei Conghui You Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i> Antioxidants <i>Escherichia coli</i> small RNA GcvB oxidative stress OxyR |
| title | Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i> |
| title_full | Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i> |
| title_fullStr | Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i> |
| title_full_unstemmed | Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i> |
| title_short | Small RNA GcvB Regulates Oxidative Stress Response of <i>Escherichia coli</i> |
| title_sort | small rna gcvb regulates oxidative stress response of i escherichia coli i |
| topic | <i>Escherichia coli</i> small RNA GcvB oxidative stress OxyR |
| url | https://www.mdpi.com/2076-3921/10/11/1774 |
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