An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards
This is the first report describing an analytical method for quantitative analysis of two naturally occurring sulphur compounds, <i>S</i>-methyl-<span style="font-variant: small-caps;">l</span>-cysteine (SMC) and <i>S</i>-methyl-<span style="font...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/13/2427 |
| _version_ | 1818469650062639104 |
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| author | Tharsini Sivapalan Antonietta Melchini Jack Coode-Bate Paul W. Needs Richard F. Mithen Shikha Saha |
| author_facet | Tharsini Sivapalan Antonietta Melchini Jack Coode-Bate Paul W. Needs Richard F. Mithen Shikha Saha |
| author_sort | Tharsini Sivapalan |
| collection | DOAJ |
| description | This is the first report describing an analytical method for quantitative analysis of two naturally occurring sulphur compounds, <i>S</i>-methyl-<span style="font-variant: small-caps;">l</span>-cysteine (SMC) and <i>S</i>-methyl-<span style="font-variant: small-caps;">l</span>-cysteine sulfoxide (SMCSO), in human body fluids using isotope-labelled internal standards and liquid chromatography-mass spectrometry (LC-MS)/MS techniques. This method was validated according to the guideline of the Royal Society of Chemistry Analytical Methods Committee. It offers significant advantages including simple and fast preparation of human biological samples. The limits of detection of SMC were 0.08 µM for urine and 0.04 µM for plasma. The limits of detection of SMCSO were 0.03 µM for urine and 0.02 µM for plasma. The calibration curves of all matrices showed linearity with correlation coefficients r<sup>2</sup> > 0.9987. The intra and inter day precisions in three levels of known concentrations were >10% and >20%, respectively. The quantification accuracy was 98.28 ± 5.66%. The proposed method would be beneficial for the rapid and accurate determination of the SMC and SMCSO in human plasma and urine samples using by isotope labelled internal standards. |
| first_indexed | 2024-04-13T21:26:26Z |
| format | Article |
| id | doaj.art-e46cb48b535249bfb08cfda523772eda |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T21:26:26Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e46cb48b535249bfb08cfda523772eda2022-12-22T02:29:18ZengMDPI AGMolecules1420-30492019-07-012413242710.3390/molecules24132427molecules24132427An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal StandardsTharsini Sivapalan0Antonietta Melchini1Jack Coode-Bate2Paul W. Needs3Richard F. Mithen4Shikha Saha5Quadram Institute Bioscience, Norwich NR4 7UQ, UKQuadram Institute Bioscience, Norwich NR4 7UQ, UKQuadram Institute Bioscience, Norwich NR4 7UQ, UKQuadram Institute Bioscience, Norwich NR4 7UQ, UKQuadram Institute Bioscience, Norwich NR4 7UQ, UKQuadram Institute Bioscience, Norwich NR4 7UQ, UKThis is the first report describing an analytical method for quantitative analysis of two naturally occurring sulphur compounds, <i>S</i>-methyl-<span style="font-variant: small-caps;">l</span>-cysteine (SMC) and <i>S</i>-methyl-<span style="font-variant: small-caps;">l</span>-cysteine sulfoxide (SMCSO), in human body fluids using isotope-labelled internal standards and liquid chromatography-mass spectrometry (LC-MS)/MS techniques. This method was validated according to the guideline of the Royal Society of Chemistry Analytical Methods Committee. It offers significant advantages including simple and fast preparation of human biological samples. The limits of detection of SMC were 0.08 µM for urine and 0.04 µM for plasma. The limits of detection of SMCSO were 0.03 µM for urine and 0.02 µM for plasma. The calibration curves of all matrices showed linearity with correlation coefficients r<sup>2</sup> > 0.9987. The intra and inter day precisions in three levels of known concentrations were >10% and >20%, respectively. The quantification accuracy was 98.28 ± 5.66%. The proposed method would be beneficial for the rapid and accurate determination of the SMC and SMCSO in human plasma and urine samples using by isotope labelled internal standards.https://www.mdpi.com/1420-3049/24/13/2427<i>S</i>-methyl-<span style="font-variant: small-caps">l</span>-cysteine<i>S</i>-methyl-<span style="font-variant: small-caps">l</span>-cysteine sulfoxidesulphur compoundsliquid chromatography tandem mass spectrometrybody fluids |
| spellingShingle | Tharsini Sivapalan Antonietta Melchini Jack Coode-Bate Paul W. Needs Richard F. Mithen Shikha Saha An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards Molecules <i>S</i>-methyl-<span style="font-variant: small-caps">l</span>-cysteine <i>S</i>-methyl-<span style="font-variant: small-caps">l</span>-cysteine sulfoxide sulphur compounds liquid chromatography tandem mass spectrometry body fluids |
| title | An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards |
| title_full | An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards |
| title_fullStr | An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards |
| title_full_unstemmed | An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards |
| title_short | An LC-MS/MS Method to Measure <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine and <i>S</i>-Methyl-<span style="font-variant: small-caps">l</span>-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards |
| title_sort | lc ms ms method to measure i s i methyl span style font variant small caps l span cysteine and i s i methyl span style font variant small caps l span cysteine sulfoxide in human specimens using isotope labelled internal standards |
| topic | <i>S</i>-methyl-<span style="font-variant: small-caps">l</span>-cysteine <i>S</i>-methyl-<span style="font-variant: small-caps">l</span>-cysteine sulfoxide sulphur compounds liquid chromatography tandem mass spectrometry body fluids |
| url | https://www.mdpi.com/1420-3049/24/13/2427 |
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