Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimurium
There are 27 million cases of Salmonella Typhimurium (STM) reported worldwide annually, which have resulted in 217,000 deaths to date. Thus, there is an urgent requirement to develop novel antibacterial agents to target the multidrug-resistant strains of STM. We evaluated the inhibitory effect of th...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222010228 |
| _version_ | 1798003161357615104 |
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| author | Yuanze Gao Helin Chen Wen Li Yu Zhang Jie Luo Ling Zhao Fei Shi Gang Ye Xiaoli He Zhiwen Xu Ling Zhu Huaqiao Tang Yinglun Li |
| author_facet | Yuanze Gao Helin Chen Wen Li Yu Zhang Jie Luo Ling Zhao Fei Shi Gang Ye Xiaoli He Zhiwen Xu Ling Zhu Huaqiao Tang Yinglun Li |
| author_sort | Yuanze Gao |
| collection | DOAJ |
| description | There are 27 million cases of Salmonella Typhimurium (STM) reported worldwide annually, which have resulted in 217,000 deaths to date. Thus, there is an urgent requirement to develop novel antibacterial agents to target the multidrug-resistant strains of STM. We evaluated the inhibitory effect of the chloroform extracts of Atractylodes chinensis (Ac-CE) on the virulence of STM in vitro and develop it as a potential antibacterial agent. First, we determined the in vitro effects of Ac-CE on STM biofilm formation, and swimming, swarming, and adhesion to mucin. Further, we evaluated the effect of Ac-CE on the adhesion and invasion of STM at the gene level. Lastly, we evaluated the inhibitory effect of Ac-CE on STM infectivity at the cellular level. Ac-CE could attenuate both the adhesion and invasion abilities of STM in vitro. At the gene level, it could inhibit the expression of flagella, pilus, biofilm, SPI-1, and SPI-2 genes, which are related to the adhesion and invasion ability of STM in cells. Ac-CE significantly downregulated the expression of inflammatory cytokines and the TLR4/MyD88/NF-κB pathway in an STM infection cell model. It also significantly recovered the expression of intestinal barrier–related genes and proteins in intestinal cells that are damaged during STM infection. Ac-CE is effective as an antivirulence agent in alleviating STM infection. Although the main components of Ac-CE were analyzed.We have not demonstrated the antivirulence effect of the active ingredients in Ac-CE. And the antivirulence effect of Ac-CE and its active ingredients warrant further in vivo studies. |
| first_indexed | 2024-04-11T12:03:28Z |
| format | Article |
| id | doaj.art-e46ce7febf214f939240495646321278 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-11T12:03:28Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-e46ce7febf214f9392404956463212782022-12-22T04:24:47ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-10-01154113633Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimuriumYuanze Gao0Helin Chen1Wen Li2Yu Zhang3Jie Luo4Ling Zhao5Fei Shi6Gang Ye7Xiaoli He8Zhiwen Xu9Ling Zhu10Huaqiao Tang11Yinglun Li12College of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaAnimal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaNational Ethnic Affairs Commission Key Open Laboratory of Traditional Chinese Veterinary Medicine, Tongren Polytechnic College, Tongren 554300, Guizhou, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaCollege of Science, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, China; Corresponding authors.College of Veterinary Medicine, Sichuan Agricultural University, 211 Huimin Road, Chengdu 611130, Sichuan, China; Corresponding authors.There are 27 million cases of Salmonella Typhimurium (STM) reported worldwide annually, which have resulted in 217,000 deaths to date. Thus, there is an urgent requirement to develop novel antibacterial agents to target the multidrug-resistant strains of STM. We evaluated the inhibitory effect of the chloroform extracts of Atractylodes chinensis (Ac-CE) on the virulence of STM in vitro and develop it as a potential antibacterial agent. First, we determined the in vitro effects of Ac-CE on STM biofilm formation, and swimming, swarming, and adhesion to mucin. Further, we evaluated the effect of Ac-CE on the adhesion and invasion of STM at the gene level. Lastly, we evaluated the inhibitory effect of Ac-CE on STM infectivity at the cellular level. Ac-CE could attenuate both the adhesion and invasion abilities of STM in vitro. At the gene level, it could inhibit the expression of flagella, pilus, biofilm, SPI-1, and SPI-2 genes, which are related to the adhesion and invasion ability of STM in cells. Ac-CE significantly downregulated the expression of inflammatory cytokines and the TLR4/MyD88/NF-κB pathway in an STM infection cell model. It also significantly recovered the expression of intestinal barrier–related genes and proteins in intestinal cells that are damaged during STM infection. Ac-CE is effective as an antivirulence agent in alleviating STM infection. Although the main components of Ac-CE were analyzed.We have not demonstrated the antivirulence effect of the active ingredients in Ac-CE. And the antivirulence effect of Ac-CE and its active ingredients warrant further in vivo studies.http://www.sciencedirect.com/science/article/pii/S0753332222010228Salmonella typhimuriumAtractylodes chinensisAdhesionInvasionIPEC-J2Inflammation |
| spellingShingle | Yuanze Gao Helin Chen Wen Li Yu Zhang Jie Luo Ling Zhao Fei Shi Gang Ye Xiaoli He Zhiwen Xu Ling Zhu Huaqiao Tang Yinglun Li Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimurium Biomedicine & Pharmacotherapy Salmonella typhimurium Atractylodes chinensis Adhesion Invasion IPEC-J2 Inflammation |
| title | Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimurium |
| title_full | Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimurium |
| title_fullStr | Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimurium |
| title_full_unstemmed | Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimurium |
| title_short | Chloroform extracts of Atractylodes chinensis inhibit the adhesion and invasion of Salmonella typhimurium |
| title_sort | chloroform extracts of atractylodes chinensis inhibit the adhesion and invasion of salmonella typhimurium |
| topic | Salmonella typhimurium Atractylodes chinensis Adhesion Invasion IPEC-J2 Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222010228 |
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