Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals
Objective: To identify the biodiversity of Nocardia species isolated from the environments of Tehran hospitals via phenotypic and PCR molecular methods. Methods: A total of 236 samples were collected from the transplantation and cancer centers of five Tehran hospitals, and the presence of Nocardia w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Acute Disease |
| Subjects: | |
| Online Access: | http://www.jadweb.org/article.asp?issn=2221-6189;year=2019;volume=8;issue=6;spage=255;epage=259;aulast=Javadi |
| _version_ | 1811213801107750912 |
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| author | Ali Javadi Mohamad Reza Pourmand Javad Hamedi Seyyed Saeed Eshraghi |
| author_facet | Ali Javadi Mohamad Reza Pourmand Javad Hamedi Seyyed Saeed Eshraghi |
| author_sort | Ali Javadi |
| collection | DOAJ |
| description | Objective: To identify the biodiversity of Nocardia species isolated from the environments of Tehran hospitals via phenotypic and PCR molecular methods.
Methods: A total of 236 samples were collected from the transplantation and cancer centers of five Tehran hospitals, and the presence of Nocardia was identified by using standard protocols. Conventional tests were used for preliminary identification, and PCR amplification of the 598 bp amplicon of the 16S rRNA and sequence analysis of 16S rRNA were performed for genus and species identification.
Results: From the 236 soil samples, 16 samples were identified as positive based on the culture tests, while 8 samples showed positive results based on the molecular method. The phylogenetic tree showed that the most frequent genus of Nocardia was related to Nocardia cyriacigeorgica.
Conclusion: The hospital environment is a potential reservoir of a diverse range of Nocardia species. Due to the strong capability of survival, these bacteria bring a threat to the health of patients. Its identification can help physicians to implement treatments and is important for healthcare centers. |
| first_indexed | 2024-04-12T05:52:18Z |
| format | Article |
| id | doaj.art-e46f56bf45cd4eb387d7874c7d47902a |
| institution | Directory Open Access Journal |
| issn | 2221-6189 2589-5516 |
| language | English |
| last_indexed | 2024-04-12T05:52:18Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-e46f56bf45cd4eb387d7874c7d47902a2022-12-22T03:45:17ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892589-55162019-01-018625525910.4103/2221-6189.272858Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitalsAli JavadiMohamad Reza PourmandJavad HamediSeyyed Saeed EshraghiObjective: To identify the biodiversity of Nocardia species isolated from the environments of Tehran hospitals via phenotypic and PCR molecular methods. Methods: A total of 236 samples were collected from the transplantation and cancer centers of five Tehran hospitals, and the presence of Nocardia was identified by using standard protocols. Conventional tests were used for preliminary identification, and PCR amplification of the 598 bp amplicon of the 16S rRNA and sequence analysis of 16S rRNA were performed for genus and species identification. Results: From the 236 soil samples, 16 samples were identified as positive based on the culture tests, while 8 samples showed positive results based on the molecular method. The phylogenetic tree showed that the most frequent genus of Nocardia was related to Nocardia cyriacigeorgica. Conclusion: The hospital environment is a potential reservoir of a diverse range of Nocardia species. Due to the strong capability of survival, these bacteria bring a threat to the health of patients. Its identification can help physicians to implement treatments and is important for healthcare centers.http://www.jadweb.org/article.asp?issn=2221-6189;year=2019;volume=8;issue=6;spage=255;epage=259;aulast=Javadinocardiatransplantation centercancer centersoil samplesmolecular identification |
| spellingShingle | Ali Javadi Mohamad Reza Pourmand Javad Hamedi Seyyed Saeed Eshraghi Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals Journal of Acute Disease nocardia transplantation center cancer center soil samples molecular identification |
| title | Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals |
| title_full | Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals |
| title_fullStr | Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals |
| title_full_unstemmed | Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals |
| title_short | Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals |
| title_sort | diversity of nocardia species isolated from transplantation and cancer centers of tehran hospitals |
| topic | nocardia transplantation center cancer center soil samples molecular identification |
| url | http://www.jadweb.org/article.asp?issn=2221-6189;year=2019;volume=8;issue=6;spage=255;epage=259;aulast=Javadi |
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