Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells
Diet can play a vital role in cancer prevention. Nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence. The purpose of this research is to examine anti-mutagenic and apoptotic effects of apigenin in human lymphoma cells. In present study human chr...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2010-10-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/4049 |
| _version_ | 1818206996397031424 |
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| author | Mehrdad Hashemi Mehdi Nouri Long Maliheh Entezari Shohreh Nafisi Hossein Nowroozii |
| author_facet | Mehrdad Hashemi Mehdi Nouri Long Maliheh Entezari Shohreh Nafisi Hossein Nowroozii |
| author_sort | Mehrdad Hashemi |
| collection | DOAJ |
| description | Diet can play a vital role in cancer prevention. Nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence. The purpose of this research is to examine anti-mutagenic and apoptotic effects of apigenin in human lymphoma cells. In present study human chronic lymphocytic leukemia (Eheb cell line) were cultured in RPMI 1640 (Sigma), supplemented with 10% fetal calf serum, penicillin-streptomycin, L-glutamine and incubated at 37 ºC for 2 days. In addition cancer cell line was treated by and apigenin and cellular vital capacity was determined by MTT assay. Then effect of apigenin in human lymphoma B cells was examined by flow cytometry techniques. The apigenin was subsequently evaluated in terms of anti-mutagenic properties by a standard reverse mutation assay (Ames test). This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100). Thus, it requires histidine from a foreign supply to ensure its growth. The aforementioned strain gives rise to reverted colonies when expose to sodium azide as a carcinogen substance. During MTT assay, human chronic lymphocytic leukemia revealed to have a meaningful cell death when compared with controls (P<0.01) Apoptosis was induced suitably after 48 hours by flow cytometry assay. In Ames test apigenin prevented the reverted mutations and the hindrance percent of apigenin was 98.17%.These results have revealed apigenin induced apoptosis in human lymphoma B cells in vitro. |
| first_indexed | 2024-12-12T04:21:54Z |
| format | Article |
| id | doaj.art-e474619a40a5478cbc0dfd842fa58de6 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-12T04:21:54Z |
| publishDate | 2010-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-e474619a40a5478cbc0dfd842fa58de62022-12-22T00:38:18ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942010-10-014854045Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia CellsMehrdad Hashemi0Mehdi Nouri Long1Maliheh Entezari2Shohreh Nafisi3Hossein Nowroozii4Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran.Department of Medicine, Islamic Azad University, Tehran medical Branch, Tehran, Iran.Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran.Department of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran, Iran. AND Research Institute for Islamic and Complementary Medicine, Iran University of Medical Science, Tehran, Iran.Research Institute for Islamic and Complementary Medicine, Iran University of Medical Science, Tehran, Iran.Diet can play a vital role in cancer prevention. Nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence. The purpose of this research is to examine anti-mutagenic and apoptotic effects of apigenin in human lymphoma cells. In present study human chronic lymphocytic leukemia (Eheb cell line) were cultured in RPMI 1640 (Sigma), supplemented with 10% fetal calf serum, penicillin-streptomycin, L-glutamine and incubated at 37 ºC for 2 days. In addition cancer cell line was treated by and apigenin and cellular vital capacity was determined by MTT assay. Then effect of apigenin in human lymphoma B cells was examined by flow cytometry techniques. The apigenin was subsequently evaluated in terms of anti-mutagenic properties by a standard reverse mutation assay (Ames test). This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100). Thus, it requires histidine from a foreign supply to ensure its growth. The aforementioned strain gives rise to reverted colonies when expose to sodium azide as a carcinogen substance. During MTT assay, human chronic lymphocytic leukemia revealed to have a meaningful cell death when compared with controls (P<0.01) Apoptosis was induced suitably after 48 hours by flow cytometry assay. In Ames test apigenin prevented the reverted mutations and the hindrance percent of apigenin was 98.17%.These results have revealed apigenin induced apoptosis in human lymphoma B cells in vitro.https://acta.tums.ac.ir/index.php/acta/article/view/4049Antimutagenicityantineoplastic agentsLeukemia |
| spellingShingle | Mehrdad Hashemi Mehdi Nouri Long Maliheh Entezari Shohreh Nafisi Hossein Nowroozii Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells Acta Medica Iranica Antimutagenicity antineoplastic agents Leukemia |
| title | Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells |
| title_full | Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells |
| title_fullStr | Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells |
| title_full_unstemmed | Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells |
| title_short | Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells |
| title_sort | anti mutagenic and pro apoptotic effects of apigenin on human chronic lymphocytic leukemia cells |
| topic | Antimutagenicity antineoplastic agents Leukemia |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/4049 |
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