The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid
Candida albicans is a resident fungus of the human intestinal microflora. Commonly isolated at low abundance in healthy people, C. albicans outcompetes local microbiota during candidiasis episodes. Under normal conditions, members of the human gastrointestinal (GI) microbiota were shown to keep C. a...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-11-01
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| Series: | G3: Genes, Genomes, Genetics |
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| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.117.300238 |
| _version_ | 1818430894729330688 |
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| author | Fabien Cottier Alrina Shin Min Tan Marina Yurieva Webber Liao Josephine Lum Michael Poidinger Francesca Zolezzi Norman Pavelka |
| author_facet | Fabien Cottier Alrina Shin Min Tan Marina Yurieva Webber Liao Josephine Lum Michael Poidinger Francesca Zolezzi Norman Pavelka |
| author_sort | Fabien Cottier |
| collection | DOAJ |
| description | Candida albicans is a resident fungus of the human intestinal microflora. Commonly isolated at low abundance in healthy people, C. albicans outcompetes local microbiota during candidiasis episodes. Under normal conditions, members of the human gastrointestinal (GI) microbiota were shown to keep C. albicans colonization under control. By releasing weak organic acids (WOAs), bacteria are able to moderate yeast growth. This mechanism displays a synergistic effect in vitro with the absence of glucose in medium of culture, which underlines the complex interactions that C. albicans faces in its natural environment. Inactivation of the transcriptional regulator MIG1 in C. albicans results in a lack of sensitivity to this synergistic outcome. To decipher C. albicans transcriptional responses to glucose, WOAs, and the role of MIG1, we performed RNA sequencing (RNA-seq) on four biological replicates exposed to combinations of these three parameters. We were able to characterize the (i) glucose response, (ii) response to acetic and butyric acid, (iii) MIG1 regulation of C. albicans, and (iv) genes responsible for WOA resistance. We identified a group of six genes linked to WOA sensitivity in a glucose-MIG1-dependent manner and inactivated one of these genes, the putative glucose transporter HGT16, in a SC5314 wild-type background. As expected, the mutant displayed a partial complementation to WOA resistance in the absence of glucose. This result points toward a mechanism of WOA sensitivity in C. albicans involving membrane transporters, which could be exploited to control yeast colonization in human body niches. |
| first_indexed | 2024-12-14T15:40:40Z |
| format | Article |
| id | doaj.art-e47667bb6a994f699a7cc6bf050161fe |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-14T15:40:40Z |
| publishDate | 2017-11-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-e47667bb6a994f699a7cc6bf050161fe2022-12-21T22:55:38ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362017-11-017113597360410.1534/g3.117.3002384The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic AcidFabien CottierAlrina Shin Min TanMarina YurievaWebber LiaoJosephine LumMichael PoidingerFrancesca ZolezziNorman PavelkaCandida albicans is a resident fungus of the human intestinal microflora. Commonly isolated at low abundance in healthy people, C. albicans outcompetes local microbiota during candidiasis episodes. Under normal conditions, members of the human gastrointestinal (GI) microbiota were shown to keep C. albicans colonization under control. By releasing weak organic acids (WOAs), bacteria are able to moderate yeast growth. This mechanism displays a synergistic effect in vitro with the absence of glucose in medium of culture, which underlines the complex interactions that C. albicans faces in its natural environment. Inactivation of the transcriptional regulator MIG1 in C. albicans results in a lack of sensitivity to this synergistic outcome. To decipher C. albicans transcriptional responses to glucose, WOAs, and the role of MIG1, we performed RNA sequencing (RNA-seq) on four biological replicates exposed to combinations of these three parameters. We were able to characterize the (i) glucose response, (ii) response to acetic and butyric acid, (iii) MIG1 regulation of C. albicans, and (iv) genes responsible for WOA resistance. We identified a group of six genes linked to WOA sensitivity in a glucose-MIG1-dependent manner and inactivated one of these genes, the putative glucose transporter HGT16, in a SC5314 wild-type background. As expected, the mutant displayed a partial complementation to WOA resistance in the absence of glucose. This result points toward a mechanism of WOA sensitivity in C. albicans involving membrane transporters, which could be exploited to control yeast colonization in human body niches.http://g3journal.org/lookup/doi/10.1534/g3.117.300238short-chain fatty acidtranscriptomicsgene expression profilingRNA-seqglucose repressionMIG1Candida albicans |
| spellingShingle | Fabien Cottier Alrina Shin Min Tan Marina Yurieva Webber Liao Josephine Lum Michael Poidinger Francesca Zolezzi Norman Pavelka The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid G3: Genes, Genomes, Genetics short-chain fatty acid transcriptomics gene expression profiling RNA-seq glucose repression MIG1 Candida albicans |
| title | The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid |
| title_full | The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid |
| title_fullStr | The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid |
| title_full_unstemmed | The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid |
| title_short | The Transcriptional Response of Candida albicans to Weak Organic Acids, Carbon Source, and MIG1 Inactivation Unveils a Role for HGT16 in Mediating the Fungistatic Effect of Acetic Acid |
| title_sort | transcriptional response of candida albicans to weak organic acids carbon source and mig1 inactivation unveils a role for hgt16 in mediating the fungistatic effect of acetic acid |
| topic | short-chain fatty acid transcriptomics gene expression profiling RNA-seq glucose repression MIG1 Candida albicans |
| url | http://g3journal.org/lookup/doi/10.1534/g3.117.300238 |
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