Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MS
The goal of this study was to identify and compare the main biomarkers of <i>Taxillus chinensis</i> from different hosts. A metabolomics approach utilizing ultra-pressure liquid chromatography coupled with tandem mass spectrometry (UPLC-MS), including cluster analysis, sample correlation...
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MDPI AG
2021-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/24/7681 |
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| author | Li Li Jianbei Teng Yilin Zhu Fengfeng Xie Jing Hou Yuan Ling Hua Zhu |
| author_facet | Li Li Jianbei Teng Yilin Zhu Fengfeng Xie Jing Hou Yuan Ling Hua Zhu |
| author_sort | Li Li |
| collection | DOAJ |
| description | The goal of this study was to identify and compare the main biomarkers of <i>Taxillus chinensis</i> from different hosts. A metabolomics approach utilizing ultra-pressure liquid chromatography coupled with tandem mass spectrometry (UPLC-MS), including cluster analysis, sample correlation analysis and orthogonal partial least squares discriminant analysis, was used to explore the flavonoid metabolites of <i>Taxillus chinensis</i> growing on different hosts. Results: The total flavonoids content (up to 30.08 mg/g) in <i>Taxillus chinensis</i> from <i>Morus alba</i> (CSG) was significantly higher than that from growth on <i>Liquidambar formosana</i> (CFG) or <i>Clausena lansium</i> (CHG) (<i>p </i>< 0.01). There were 23 different metabolites between CSG and CHG, 23 different metabolites between CSG and CFG, and 19 different metabolites between CHG and CFG. The results demonstrated that different hosts exerted a large influence on the metabolites of <i>Taxillus chinensis</i>; it was found that CSG differed from CFG and CHG in eleven metabolic compounds, ten of which were upregulated and one of which was downregulated. Most of these metabolites derive from compounds contained in the host plant, white mulberry (<i>Morus alba</i>); many feature potent anti-cancer effects. Differences in host can influence the type and abundance of flavonoids in parasitic plants such as <i>Taxillus chinensis</i>, which is of great significance to researchers seeking to understand the formation mechanism of <i>Taxillus chinensis</i> metabolites. Therefore, attention should be paid to the species of host plant when studying the <i>Taxillus chinensis</i> metabolome. Plants grown on <i>Morus alba</i> offer the greatest potential for the development of new anti-cancer drugs. |
| first_indexed | 2024-03-10T03:28:47Z |
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| language | English |
| last_indexed | 2024-03-10T03:28:47Z |
| publishDate | 2021-12-01 |
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| series | Molecules |
| spelling | doaj.art-e47900b92fca413c9dcf2ae1717d2fcf2023-11-23T09:47:43ZengMDPI AGMolecules1420-30492021-12-012624768110.3390/molecules26247681Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MSLi Li0Jianbei Teng1Yilin Zhu2Fengfeng Xie3Jing Hou4Yuan Ling5Hua Zhu6College of Pharmacy, Chengdu University of TCM, Chengdu 611137, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Chengdu University of TCM, Chengdu 611137, ChinaThe goal of this study was to identify and compare the main biomarkers of <i>Taxillus chinensis</i> from different hosts. A metabolomics approach utilizing ultra-pressure liquid chromatography coupled with tandem mass spectrometry (UPLC-MS), including cluster analysis, sample correlation analysis and orthogonal partial least squares discriminant analysis, was used to explore the flavonoid metabolites of <i>Taxillus chinensis</i> growing on different hosts. Results: The total flavonoids content (up to 30.08 mg/g) in <i>Taxillus chinensis</i> from <i>Morus alba</i> (CSG) was significantly higher than that from growth on <i>Liquidambar formosana</i> (CFG) or <i>Clausena lansium</i> (CHG) (<i>p </i>< 0.01). There were 23 different metabolites between CSG and CHG, 23 different metabolites between CSG and CFG, and 19 different metabolites between CHG and CFG. The results demonstrated that different hosts exerted a large influence on the metabolites of <i>Taxillus chinensis</i>; it was found that CSG differed from CFG and CHG in eleven metabolic compounds, ten of which were upregulated and one of which was downregulated. Most of these metabolites derive from compounds contained in the host plant, white mulberry (<i>Morus alba</i>); many feature potent anti-cancer effects. Differences in host can influence the type and abundance of flavonoids in parasitic plants such as <i>Taxillus chinensis</i>, which is of great significance to researchers seeking to understand the formation mechanism of <i>Taxillus chinensis</i> metabolites. Therefore, attention should be paid to the species of host plant when studying the <i>Taxillus chinensis</i> metabolome. Plants grown on <i>Morus alba</i> offer the greatest potential for the development of new anti-cancer drugs.https://www.mdpi.com/1420-3049/26/24/7681<i>Taxillus chinensis</i>flavonoid metabolitesPCAOPLS-DAUPLC-ESI-MS/MS |
| spellingShingle | Li Li Jianbei Teng Yilin Zhu Fengfeng Xie Jing Hou Yuan Ling Hua Zhu Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MS Molecules <i>Taxillus chinensis</i> flavonoid metabolites PCA OPLS-DA UPLC-ESI-MS/MS |
| title | Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MS |
| title_full | Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MS |
| title_fullStr | Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MS |
| title_full_unstemmed | Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MS |
| title_short | Metabolomics Study of Flavonoids of <em>Taxillus</em><em>chinensis</em> on Different Hosts Using UPLC-ESI-MS/MS |
| title_sort | metabolomics study of flavonoids of em taxillus em em chinensis em on different hosts using uplc esi ms ms |
| topic | <i>Taxillus chinensis</i> flavonoid metabolites PCA OPLS-DA UPLC-ESI-MS/MS |
| url | https://www.mdpi.com/1420-3049/26/24/7681 |
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