Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection
IntroductionPeanut (Arachis hypogaea L.) is an important cash crop worldwide. Compared with the ordinary peanut with pure pink testa, peanut with variegated testa color has attractive appearance and a higher market value. In addition, the variegated testa represents a distinct regulation pattern of...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1145098/full |
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| author | Hao Chen Hao Chen Xinlei Yang Rirong Xu Xiangyu Chen Haifeng Zhong Nian Liu Li Huang Huaiyong Luo Dongxin Huai Wenjing Liu Yuhua Chen Jianhong Chen Huifang Jiang |
| author_facet | Hao Chen Hao Chen Xinlei Yang Rirong Xu Xiangyu Chen Haifeng Zhong Nian Liu Li Huang Huaiyong Luo Dongxin Huai Wenjing Liu Yuhua Chen Jianhong Chen Huifang Jiang |
| author_sort | Hao Chen |
| collection | DOAJ |
| description | IntroductionPeanut (Arachis hypogaea L.) is an important cash crop worldwide. Compared with the ordinary peanut with pure pink testa, peanut with variegated testa color has attractive appearance and a higher market value. In addition, the variegated testa represents a distinct regulation pattern of anthocyanin accumulation in integument cells.MethodsIn order to identify the genetic locus underlying variegated testa color in peanut, two populations were constructed from the crosses between Fuhua 8 (pure-pink testa) and Wucai (red on white variegated testa), Quanhonghua 1 (pure-red testa) and Wucai, respectively. Genetic analysis and bulked sergeant analysis sequencing were applied to detect and identify the genetic locus for variegated testa color. Marker-assisted selection was used to develop new variegated testa peanut lines.ResultsAs a result, all the seeds harvested from the F1 individuals of both populations showed the variegated testa type with white trace. Genetic analysis revealed that the pigmentation of colored region in red on white variegated testa was controlled by a previous reported gene AhRt1, while the formation of white region (un-pigmented region) in variegated testa was controlled by another single genetic locus. This locus, named as AhVt1 (Arachis hypogaea Variegated Testa 1), was preliminary mapped on chromosome 08 through bulked sergeant analysis sequencing. Using a secondary mapping population derived from the cross between Fuhua 8 and Wucai, AhVt1 was further mapped to a 1.89-Mb genomic interval by linkage analysis, and several potential genes associated with the uneven distribution of anthocyanin, such as MADS-box, MYB, and Chalcone synthase-like protein, were harbored in the region. Moreover, the molecular markers closely linked to the AhVt1 were developed, and the new variegated testa peanut lines were obtained with the help of marker-assisted selection.ConclusionOur findings will accelerate the breeding program for developing new peanut varieties with “colorful” testa colors and laid a foundation for map-based cloning of gene responsible for variegated testa. |
| first_indexed | 2024-04-09T23:37:45Z |
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| spelling | doaj.art-e47ae74104834f44b86d9d5ecdf745602023-03-20T05:17:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11450981145098Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selectionHao Chen0Hao Chen1Xinlei Yang2Rirong Xu3Xiangyu Chen4Haifeng Zhong5Nian Liu6Li Huang7Huaiyong Luo8Dongxin Huai9Wenjing Liu10Yuhua Chen11Jianhong Chen12Huifang Jiang13Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fujian Research Station of Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Wuhan, ChinaState Key Laboratory of North China Crop Improvement and Regulation, North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory for Crop Germplasm Resources of Hebei, Hebei Agricultural University, Baoding, ChinaInstitute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fujian Research Station of Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaInstitute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fujian Research Station of Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaInstitute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fujian Research Station of Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Wuhan, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Wuhan, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Wuhan, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Wuhan, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fujian Key Laboratory of Agro-products Quality and Safety, Fuzhou, ChinaInstitute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fujian Research Station of Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, ChinaR&D Center for Oil Crops, Quanzhou Institute of Agricultural Sciences, Jinjiang, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Wuhan, ChinaIntroductionPeanut (Arachis hypogaea L.) is an important cash crop worldwide. Compared with the ordinary peanut with pure pink testa, peanut with variegated testa color has attractive appearance and a higher market value. In addition, the variegated testa represents a distinct regulation pattern of anthocyanin accumulation in integument cells.MethodsIn order to identify the genetic locus underlying variegated testa color in peanut, two populations were constructed from the crosses between Fuhua 8 (pure-pink testa) and Wucai (red on white variegated testa), Quanhonghua 1 (pure-red testa) and Wucai, respectively. Genetic analysis and bulked sergeant analysis sequencing were applied to detect and identify the genetic locus for variegated testa color. Marker-assisted selection was used to develop new variegated testa peanut lines.ResultsAs a result, all the seeds harvested from the F1 individuals of both populations showed the variegated testa type with white trace. Genetic analysis revealed that the pigmentation of colored region in red on white variegated testa was controlled by a previous reported gene AhRt1, while the formation of white region (un-pigmented region) in variegated testa was controlled by another single genetic locus. This locus, named as AhVt1 (Arachis hypogaea Variegated Testa 1), was preliminary mapped on chromosome 08 through bulked sergeant analysis sequencing. Using a secondary mapping population derived from the cross between Fuhua 8 and Wucai, AhVt1 was further mapped to a 1.89-Mb genomic interval by linkage analysis, and several potential genes associated with the uneven distribution of anthocyanin, such as MADS-box, MYB, and Chalcone synthase-like protein, were harbored in the region. Moreover, the molecular markers closely linked to the AhVt1 were developed, and the new variegated testa peanut lines were obtained with the help of marker-assisted selection.ConclusionOur findings will accelerate the breeding program for developing new peanut varieties with “colorful” testa colors and laid a foundation for map-based cloning of gene responsible for variegated testa.https://www.frontiersin.org/articles/10.3389/fpls.2023.1145098/fullvariegated testa colorBSA-seqpeanut (Arachis hypogaea L)genetic mappingmarker- assisted selection |
| spellingShingle | Hao Chen Hao Chen Xinlei Yang Rirong Xu Xiangyu Chen Haifeng Zhong Nian Liu Li Huang Huaiyong Luo Dongxin Huai Wenjing Liu Yuhua Chen Jianhong Chen Huifang Jiang Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection Frontiers in Plant Science variegated testa color BSA-seq peanut (Arachis hypogaea L) genetic mapping marker- assisted selection |
| title | Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection |
| title_full | Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection |
| title_fullStr | Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection |
| title_full_unstemmed | Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection |
| title_short | Genetic mapping of AhVt1, a novel genetic locus that confers the variegated testa color in cultivated peanut (Arachis hypogaea L.) and its utilization for marker-assisted selection |
| title_sort | genetic mapping of ahvt1 a novel genetic locus that confers the variegated testa color in cultivated peanut arachis hypogaea l and its utilization for marker assisted selection |
| topic | variegated testa color BSA-seq peanut (Arachis hypogaea L) genetic mapping marker- assisted selection |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1145098/full |
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