Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics
Purpose: To analyze human and bacteria proteomic profiles in bile, exposed to a tumor vs. non-tumor microenvironment, in order to identify differences between these conditions, which may contribute to a better understanding of pancreatic carcinogenesis.Patients and Methods: Using liquid chromatograp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.01032/full |
| _version_ | 1819092344304041984 |
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| author | Ariel A. Arteta Ariel A. Arteta Ariel A. Arteta Miryan Sánchez-Jiménez Diego F. Dávila Oscar G. Palacios Nora Cardona-Castro Nora Cardona-Castro Nora Cardona-Castro |
| author_facet | Ariel A. Arteta Ariel A. Arteta Ariel A. Arteta Miryan Sánchez-Jiménez Diego F. Dávila Oscar G. Palacios Nora Cardona-Castro Nora Cardona-Castro Nora Cardona-Castro |
| author_sort | Ariel A. Arteta |
| collection | DOAJ |
| description | Purpose: To analyze human and bacteria proteomic profiles in bile, exposed to a tumor vs. non-tumor microenvironment, in order to identify differences between these conditions, which may contribute to a better understanding of pancreatic carcinogenesis.Patients and Methods: Using liquid chromatography and mass spectrometry, human and bacterial proteomic profiles of a total of 20 bile samples (7 from gallstone (GS) patients, and 13 from pancreatic head ductal adenocarcinoma (PDAC) patients) that were collected during surgery and taken directly from the gallbladder, were compared. g:Profiler and KEGG (Kyoto Encyclopedia of Genes and Genomes) Mapper Reconstruct Pathway were used as the main comparative platform focusing on over-represented biological pathways among human proteins and interaction pathways among bacterial proteins.Results: Three bacterial infection pathways were over-represented in the human PDAC group of proteins. IL-8 is the only human protein that coincides in the three pathways and this protein is only present in the PDAC group. Quantitative and qualitative differences in bacterial proteins suggest a dysbiotic microenvironment in the PDAC group, supported by significant participation of antibiotic biosynthesis enzymes. Prokaryotes interaction signaling pathways highlight the presence of zeatin in the GS group and surfactin in the PDAC group, the former in the metabolism of terpenoids and polyketides, and the latter in both metabolisms of terpenoids, polyketides and quorum sensing. Based on our findings, we propose a bacterial-induced carcinogenesis model for the biliary tract.Conclusion: To the best of our knowledge this is the first study with the aim of comparing human and bacterial bile proteins in a tumor vs. non-tumor microenvironment. We proposed a new carcinogenesis model for the biliary tract based on bile metaproteomic findings. Our results suggest that bacteria may be key players in biliary tract carcinogenesis, in a long-lasting dysbiotic and epithelially harmful microenvironment, in which specific bacterial species' biofilm formation is of utmost importance. Our finding should be further explored in future using in vitro and in vivo investigations. |
| first_indexed | 2024-12-21T22:54:07Z |
| format | Article |
| id | doaj.art-e47b243ca8cb4edcb5c14b535eca38cf |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-21T22:54:07Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-e47b243ca8cb4edcb5c14b535eca38cf2022-12-21T18:47:29ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-07-011010.3389/fonc.2020.01032532862Biliary Tract Carcinogenesis Model Based on Bile MetaproteomicsAriel A. Arteta0Ariel A. Arteta1Ariel A. Arteta2Miryan Sánchez-Jiménez3Diego F. Dávila4Oscar G. Palacios5Nora Cardona-Castro6Nora Cardona-Castro7Nora Cardona-Castro8School of Graduate Studies, CES University, Medellín, ColombiaBasic Science Research Group, School of Medicine, CES University, Medellín, ColombiaAssociated Professor Department of Pathology, University of Antioquia, Medellín, ColombiaColombian Institute of Tropical Medicine (ICMT), Sabaneta, ColombiaDepartment of Hepatobiliary and Pancreatic Surgery, CES Clinic, Medellín, ColombiaDepartment of Hepatobiliary and Pancreatic Surgery, CES Clinic, Medellín, ColombiaSchool of Graduate Studies, CES University, Medellín, ColombiaBasic Science Research Group, School of Medicine, CES University, Medellín, ColombiaColombian Institute of Tropical Medicine (ICMT), Sabaneta, ColombiaPurpose: To analyze human and bacteria proteomic profiles in bile, exposed to a tumor vs. non-tumor microenvironment, in order to identify differences between these conditions, which may contribute to a better understanding of pancreatic carcinogenesis.Patients and Methods: Using liquid chromatography and mass spectrometry, human and bacterial proteomic profiles of a total of 20 bile samples (7 from gallstone (GS) patients, and 13 from pancreatic head ductal adenocarcinoma (PDAC) patients) that were collected during surgery and taken directly from the gallbladder, were compared. g:Profiler and KEGG (Kyoto Encyclopedia of Genes and Genomes) Mapper Reconstruct Pathway were used as the main comparative platform focusing on over-represented biological pathways among human proteins and interaction pathways among bacterial proteins.Results: Three bacterial infection pathways were over-represented in the human PDAC group of proteins. IL-8 is the only human protein that coincides in the three pathways and this protein is only present in the PDAC group. Quantitative and qualitative differences in bacterial proteins suggest a dysbiotic microenvironment in the PDAC group, supported by significant participation of antibiotic biosynthesis enzymes. Prokaryotes interaction signaling pathways highlight the presence of zeatin in the GS group and surfactin in the PDAC group, the former in the metabolism of terpenoids and polyketides, and the latter in both metabolisms of terpenoids, polyketides and quorum sensing. Based on our findings, we propose a bacterial-induced carcinogenesis model for the biliary tract.Conclusion: To the best of our knowledge this is the first study with the aim of comparing human and bacterial bile proteins in a tumor vs. non-tumor microenvironment. We proposed a new carcinogenesis model for the biliary tract based on bile metaproteomic findings. Our results suggest that bacteria may be key players in biliary tract carcinogenesis, in a long-lasting dysbiotic and epithelially harmful microenvironment, in which specific bacterial species' biofilm formation is of utmost importance. Our finding should be further explored in future using in vitro and in vivo investigations.https://www.frontiersin.org/article/10.3389/fonc.2020.01032/fullpancreatic cancermetaproteomicproteomicbilezeatinsurfactin |
| spellingShingle | Ariel A. Arteta Ariel A. Arteta Ariel A. Arteta Miryan Sánchez-Jiménez Diego F. Dávila Oscar G. Palacios Nora Cardona-Castro Nora Cardona-Castro Nora Cardona-Castro Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics Frontiers in Oncology pancreatic cancer metaproteomic proteomic bile zeatin surfactin |
| title | Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics |
| title_full | Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics |
| title_fullStr | Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics |
| title_full_unstemmed | Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics |
| title_short | Biliary Tract Carcinogenesis Model Based on Bile Metaproteomics |
| title_sort | biliary tract carcinogenesis model based on bile metaproteomics |
| topic | pancreatic cancer metaproteomic proteomic bile zeatin surfactin |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.01032/full |
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