Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach
Allicin (diallylthiosulfinate) is the principal organosulfur compound present in freshly damaged garlic tissue which exhibits a wide range of biological actions including antibacterial, antifungal, antiviral and anticancer properties. The antifungal activities of allicin were investigated against pl...
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MDPI AG
2020-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/7/2563 |
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| author | Muhammad Sarfraz Muhammad Jawad Nasim Claus Jacob Martin C. H. Gruhlke |
| author_facet | Muhammad Sarfraz Muhammad Jawad Nasim Claus Jacob Martin C. H. Gruhlke |
| author_sort | Muhammad Sarfraz |
| collection | DOAJ |
| description | Allicin (diallylthiosulfinate) is the principal organosulfur compound present in freshly damaged garlic tissue which exhibits a wide range of biological actions including antibacterial, antifungal, antiviral and anticancer properties. The antifungal activities of allicin were investigated against plant pathogenic fungi of agriculture importance. Furthermore, a yeast genome haploinsufficiency screening was also employed to decipher the antifungal mode of action of allicin. Wildtype and 1152 yeast mutant strains (each deprived of one specific allele of an essential gene in a diploid strain) were screened against allicin. Allicin exhibited promising antifungal properties against all the tested plant pathogens. Haploinsufficiency screening revealed three hypersensitive yeast mutants with gene deletions coding for proteins involved in DNA replication, mitochondrial translation and chromatids cohesion. These processes play a vital role in the cell cycle, growth and viability of yeast cells. Taken together, the results of the present study unravel the excellent antifungal activities and mechanisms and modes of action of allicin. These findings also indicate the potential use of allicin as an alternative “green” fungicide (fumigant) in agriculture. |
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| format | Article |
| id | doaj.art-e47cb9beb1f74fc3a150164f4daece5e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:36:19Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-e47cb9beb1f74fc3a150164f4daece5e2023-11-19T21:01:28ZengMDPI AGApplied Sciences2076-34172020-04-01107256310.3390/app10072563Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling ApproachMuhammad Sarfraz0Muhammad Jawad Nasim1Claus Jacob2Martin C. H. Gruhlke3Department of Plant Physiology, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, GermanyDepartment of Plant Physiology, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, GermanyDivision of Bioorganic Chemistry, School of Pharmacy, Campus B2 1, University of Saarland, D-66123 Saarbruecken, GermanyDepartment of Plant Physiology, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, GermanyAllicin (diallylthiosulfinate) is the principal organosulfur compound present in freshly damaged garlic tissue which exhibits a wide range of biological actions including antibacterial, antifungal, antiviral and anticancer properties. The antifungal activities of allicin were investigated against plant pathogenic fungi of agriculture importance. Furthermore, a yeast genome haploinsufficiency screening was also employed to decipher the antifungal mode of action of allicin. Wildtype and 1152 yeast mutant strains (each deprived of one specific allele of an essential gene in a diploid strain) were screened against allicin. Allicin exhibited promising antifungal properties against all the tested plant pathogens. Haploinsufficiency screening revealed three hypersensitive yeast mutants with gene deletions coding for proteins involved in DNA replication, mitochondrial translation and chromatids cohesion. These processes play a vital role in the cell cycle, growth and viability of yeast cells. Taken together, the results of the present study unravel the excellent antifungal activities and mechanisms and modes of action of allicin. These findings also indicate the potential use of allicin as an alternative “green” fungicide (fumigant) in agriculture.https://www.mdpi.com/2076-3417/10/7/2563allicingarlicantifungal activitieshaploinsufficiency screeningyeastchemogenetic screening |
| spellingShingle | Muhammad Sarfraz Muhammad Jawad Nasim Claus Jacob Martin C. H. Gruhlke Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach Applied Sciences allicin garlic antifungal activities haploinsufficiency screening yeast chemogenetic screening |
| title | Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach |
| title_full | Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach |
| title_fullStr | Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach |
| title_full_unstemmed | Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach |
| title_short | Efficacy of Allicin against Plant Pathogenic Fungi and Unveiling the Underlying Mode of Action Employing Yeast Based Chemogenetic Profiling Approach |
| title_sort | efficacy of allicin against plant pathogenic fungi and unveiling the underlying mode of action employing yeast based chemogenetic profiling approach |
| topic | allicin garlic antifungal activities haploinsufficiency screening yeast chemogenetic screening |
| url | https://www.mdpi.com/2076-3417/10/7/2563 |
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