Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry
Purpose: To investigate, for the first time, the chemical composition of essential oil of the tubers and leaves of Jerusalem artichoke (Helianthus tuberosus L.), a species of sunflower native to eastern North America, growing in Ukraine. Methods: A hydrodistillation apparatus was used for the extrac...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2014-12-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-521.pdf |
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| author | Zead Helmi Khaldun Mohammad Al Azzam Yuliya Tsymbalista Refat Abo Ghazleh Hassan Shaibah Hassan Aboul-Enein |
| author_facet | Zead Helmi Khaldun Mohammad Al Azzam Yuliya Tsymbalista Refat Abo Ghazleh Hassan Shaibah Hassan Aboul-Enein |
| author_sort | Zead Helmi |
| collection | DOAJ |
| description | Purpose: To investigate, for the first time, the chemical composition of
essential oil of the tubers and leaves of Jerusalem artichoke
(Helianthus tuberosus L.), a species of sunflower native
to eastern North America, growing in Ukraine.
Methods: A hydrodistillation apparatus was used for the extraction of
volatile components and then it was analysed by gas chromatography
equipped with a split-splitless injector (split ratio, 1:50) and flame
ionization detector (FID). The oil was analyzed under linear temperature
programming applied at 4°C/min from 50°C - 340°C. Temperatures of the
injector and FID detector were maintained at 280°C and 300°C,
respectively. The chemical analysis of the oil was carried out using gas
chromatography coupled to mass spectrometry (GC-MS), to determine the
chemical composition of the volatile fraction.
Results: The essential oils content ranged from 0.00019 to 0.03486 and
0.00011 to 0.00205 (g/100g), in leaves and tubers, respectively. The
qualitative and quantitative analysis led to the identification of 17
components in both species samples. The major component found in leaves
and tubers was (-)-β-bisabolene with 70.7% and 63.1%, respectively.
Conclusion:> Essential oil profile of Jerusalem artichoke species
showed significant differences between leaves and tubers species.
Additionally, the leaves of Jerusalem artichoke are a promising source
of natural β-bisabolene. |
| first_indexed | 2024-12-14T01:07:49Z |
| format | Article |
| id | doaj.art-e47d9d9b6d3c40ddad45a81d2052a5b3 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-14T01:07:49Z |
| publishDate | 2014-12-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-e47d9d9b6d3c40ddad45a81d2052a5b32022-12-21T23:22:54ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082014-12-014652152610.5681/apb.2014.077APB_1387_20140419213639Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass SpectrometryZead Helmi0Khaldun Mohammad Al Azzam1Yuliya Tsymbalista2Refat Abo Ghazleh3Hassan Shaibah4Hassan Aboul-Enein5 Faculty of Pharmacy, Isra University, 11622 Amman, Jordan. Faculty of Pharmacy, Isra University, 11622 Amman, Jordan. Department of pharmacognosy and botany, National Medical University is the name of O.O.Bogomolets, Ukraine. Pharmacy program, Batterjee Medical College for Science and Technology (BMC), 21442 Jeddah, Kingdom of Saudi Arabia. Pharmacy program, Batterjee Medical College for Science and Technology (BMC), 21442 Jeddah, Kingdom of Saudi Arabia. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Tahrir Street, Dokki, Cairo 12311, Egypt.Purpose: To investigate, for the first time, the chemical composition of essential oil of the tubers and leaves of Jerusalem artichoke (Helianthus tuberosus L.), a species of sunflower native to eastern North America, growing in Ukraine. Methods: A hydrodistillation apparatus was used for the extraction of volatile components and then it was analysed by gas chromatography equipped with a split-splitless injector (split ratio, 1:50) and flame ionization detector (FID). The oil was analyzed under linear temperature programming applied at 4°C/min from 50°C - 340°C. Temperatures of the injector and FID detector were maintained at 280°C and 300°C, respectively. The chemical analysis of the oil was carried out using gas chromatography coupled to mass spectrometry (GC-MS), to determine the chemical composition of the volatile fraction. Results: The essential oils content ranged from 0.00019 to 0.03486 and 0.00011 to 0.00205 (g/100g), in leaves and tubers, respectively. The qualitative and quantitative analysis led to the identification of 17 components in both species samples. The major component found in leaves and tubers was (-)-β-bisabolene with 70.7% and 63.1%, respectively. Conclusion:> Essential oil profile of Jerusalem artichoke species showed significant differences between leaves and tubers species. Additionally, the leaves of Jerusalem artichoke are a promising source of natural β-bisabolene.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-521.pdfEssential oilHelianthus tuberosus L.Jerusalem artichokeLeavesTubersGas chromatography |
| spellingShingle | Zead Helmi Khaldun Mohammad Al Azzam Yuliya Tsymbalista Refat Abo Ghazleh Hassan Shaibah Hassan Aboul-Enein Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry Advanced Pharmaceutical Bulletin Essential oil Helianthus tuberosus L. Jerusalem artichoke Leaves Tubers Gas chromatography |
| title | Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry |
| title_full | Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry |
| title_fullStr | Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry |
| title_full_unstemmed | Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry |
| title_short | Analysis of Essential Oil in Jerusalem Artichoke (Helianthus tuberosus L.) Leaves and Tubers by Gas Chromatography-Mass Spectrometry |
| title_sort | analysis of essential oil in jerusalem artichoke helianthus tuberosus l leaves and tubers by gas chromatography mass spectrometry |
| topic | Essential oil Helianthus tuberosus L. Jerusalem artichoke Leaves Tubers Gas chromatography |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-521.pdf |
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