Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> Cultures
Multidrug resistant bacteria are one of the most serious public health threats nowadays. How bacteria, as a population, react to the presence of antibiotics is of major importance to the outcome of the chosen treatment. In this study we addressed the impact of oxacillin, a β-lactam, the most clinica...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Fluids |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5521/5/2/76 |
| _version_ | 1797567648897171456 |
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| author | Raquel Portela Filipe Valcovo Pedro L. Almeida Rita G. Sobral Catarina R. Leal |
| author_facet | Raquel Portela Filipe Valcovo Pedro L. Almeida Rita G. Sobral Catarina R. Leal |
| author_sort | Raquel Portela |
| collection | DOAJ |
| description | Multidrug resistant bacteria are one of the most serious public health threats nowadays. How bacteria, as a population, react to the presence of antibiotics is of major importance to the outcome of the chosen treatment. In this study we addressed the impact of oxacillin, a β-lactam, the most clinically relevant class of antibiotics, in the viscosity profile of the methicillin resistant <i>Staphylococcus aureus</i> (MRSA) strain COL. In the first approach, the antibiotic was added, at concentrations under the minimum inhibitory concentration (sub-MIC), to the culture of <i>S. aureus</i> and steady-state shear flow curves were obtained for discrete time points during the bacterial growth, with and without the presence of the antibiotic, showing distinct viscosity progress over time. The different behaviors obtained led us to test the impact of the sub-inhibitory concentration and a concentration that inhibited growth. In the second approach, the viscosity growth curves were measured at a constant shear rate of 10 s<sup>−1</sup>, over time. The obtained rheological behaviors revealed distinctive characteristics associated to the presence of each concentration of the tested antibiotic. These results bring new insights to the bacteria response to a well-known bacteriolytic antibiotic. |
| first_indexed | 2024-03-10T19:45:12Z |
| format | Article |
| id | doaj.art-e483451642bc4c2f8d8dad0c49517750 |
| institution | Directory Open Access Journal |
| issn | 2311-5521 |
| language | English |
| last_indexed | 2024-03-10T19:45:12Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fluids |
| spelling | doaj.art-e483451642bc4c2f8d8dad0c495177502023-11-20T00:52:41ZengMDPI AGFluids2311-55212020-05-01527610.3390/fluids5020076Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> CulturesRaquel Portela0Filipe Valcovo1Pedro L. Almeida2Rita G. Sobral3Catarina R. Leal4UCIBIO@REQUIMTE, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, PortugalUCIBIO@REQUIMTE, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, PortugalÁrea Departamental de Física, ISEL—Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, 1959-007 Lisboa, PortugalUCIBIO@REQUIMTE, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, PortugalÁrea Departamental de Física, ISEL—Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, 1959-007 Lisboa, PortugalMultidrug resistant bacteria are one of the most serious public health threats nowadays. How bacteria, as a population, react to the presence of antibiotics is of major importance to the outcome of the chosen treatment. In this study we addressed the impact of oxacillin, a β-lactam, the most clinically relevant class of antibiotics, in the viscosity profile of the methicillin resistant <i>Staphylococcus aureus</i> (MRSA) strain COL. In the first approach, the antibiotic was added, at concentrations under the minimum inhibitory concentration (sub-MIC), to the culture of <i>S. aureus</i> and steady-state shear flow curves were obtained for discrete time points during the bacterial growth, with and without the presence of the antibiotic, showing distinct viscosity progress over time. The different behaviors obtained led us to test the impact of the sub-inhibitory concentration and a concentration that inhibited growth. In the second approach, the viscosity growth curves were measured at a constant shear rate of 10 s<sup>−1</sup>, over time. The obtained rheological behaviors revealed distinctive characteristics associated to the presence of each concentration of the tested antibiotic. These results bring new insights to the bacteria response to a well-known bacteriolytic antibiotic.https://www.mdpi.com/2311-5521/5/2/76rheologyMRSA<i>S. aureus</i>antibioticsoxacillinbactericidal |
| spellingShingle | Raquel Portela Filipe Valcovo Pedro L. Almeida Rita G. Sobral Catarina R. Leal Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> Cultures Fluids rheology MRSA <i>S. aureus</i> antibiotics oxacillin bactericidal |
| title | Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> Cultures |
| title_full | Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> Cultures |
| title_fullStr | Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> Cultures |
| title_full_unstemmed | Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> Cultures |
| title_short | Antibiotic Activity Screened by the Rheology of <i>S. aureus</i> Cultures |
| title_sort | antibiotic activity screened by the rheology of i s aureus i cultures |
| topic | rheology MRSA <i>S. aureus</i> antibiotics oxacillin bactericidal |
| url | https://www.mdpi.com/2311-5521/5/2/76 |
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