A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory Responses
For food quality and safety issues, the emergence of foodborne pathogenic bacteria has further accelerated the spread of antibiotic residues and drug resistance genes. To alleviate the harm caused by bacterial infections, it is necessary to seek novel antimicrobial agents as biopreservatives to prev...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/24/13580 |
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| author | Ming Kuang Haitao Yu Shiyan Qiao Tao Huang Jiaqi Zhang Mingchao Sun Xiumei Shi Han Chen |
| author_facet | Ming Kuang Haitao Yu Shiyan Qiao Tao Huang Jiaqi Zhang Mingchao Sun Xiumei Shi Han Chen |
| author_sort | Ming Kuang |
| collection | DOAJ |
| description | For food quality and safety issues, the emergence of foodborne pathogenic bacteria has further accelerated the spread of antibiotic residues and drug resistance genes. To alleviate the harm caused by bacterial infections, it is necessary to seek novel antimicrobial agents as biopreservatives to prevent microbial spoilage. Nanoantimicrobials have been widely used in the direct treatment of bacterial infections. CNMs, formed by chitosan nanoparticles and peptides, are promising antibiotic alternatives for use as excellent new antibacterial drugs against pathogenic bacteria. Herein, the current study evaluated the function of CNMs in the protection of foodborne pathogen <i>Escherichia coli</i> (<i>E. coli</i>) O157 infection using an intestinal epithelial cell model. Antibacterial activity assays indicated that CNMs exerted excellent bactericidal activity against <i>E. coli O157</i>. Assessment of the cytotoxicity risks toward cells demonstrated that 0.0125–0.02% of CNMs did not cause toxicity, but 0.4% of CNMs caused cytotoxicity. Additionally, CNMs did not induced genotoxicity either. CNMs protected against <i>E. coli O157</i>-induced barrier dysfunction by increasing transepithelial electrical resistance, decreasing lactate dehydrogenase and promoting the protein expression of occludin. CNMs were further found to ameliorate inflammation via modulation of tumor factor α, toll-like receptor 4 and nuclear factor κB (NF-κB) expression via inhibition of mitogen-activated protein kinase and NF-κB activation and improved antioxidant activity. Taken together, CNMs could protect the host against <i>E. coli O157</i>-induced intestinal barrier damage and inflammation, showing that CNMs have great advantages and potential application as novel antimicrobial polymers in the food industry as food biopreservatives, bringing new hope for the treatment of bacterial infections. |
| first_indexed | 2024-03-10T03:55:08Z |
| format | Article |
| id | doaj.art-e48825c083574f0eb922a929a0210c4d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:55:08Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e48825c083574f0eb922a929a0210c4d2023-11-23T08:48:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122241358010.3390/ijms222413580A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory ResponsesMing Kuang0Haitao Yu1Shiyan Qiao2Tao Huang3Jiaqi Zhang4Mingchao Sun5Xiumei Shi6Han Chen7Institute of Systems Biomedicine, Department of Immunology, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing 100191, ChinaInstitute of Systems Biomedicine, Department of Immunology, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing 100191, ChinaState Key Laboratory of Animal Nutrition, Ministry of Agriculture and Rural Affairs Feed Industry Center, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Urban Hydrological Cycle and Sponge City Technology, College of Water Sciences, Beijing Normal University, Beijing 100875, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaFor food quality and safety issues, the emergence of foodborne pathogenic bacteria has further accelerated the spread of antibiotic residues and drug resistance genes. To alleviate the harm caused by bacterial infections, it is necessary to seek novel antimicrobial agents as biopreservatives to prevent microbial spoilage. Nanoantimicrobials have been widely used in the direct treatment of bacterial infections. CNMs, formed by chitosan nanoparticles and peptides, are promising antibiotic alternatives for use as excellent new antibacterial drugs against pathogenic bacteria. Herein, the current study evaluated the function of CNMs in the protection of foodborne pathogen <i>Escherichia coli</i> (<i>E. coli</i>) O157 infection using an intestinal epithelial cell model. Antibacterial activity assays indicated that CNMs exerted excellent bactericidal activity against <i>E. coli O157</i>. Assessment of the cytotoxicity risks toward cells demonstrated that 0.0125–0.02% of CNMs did not cause toxicity, but 0.4% of CNMs caused cytotoxicity. Additionally, CNMs did not induced genotoxicity either. CNMs protected against <i>E. coli O157</i>-induced barrier dysfunction by increasing transepithelial electrical resistance, decreasing lactate dehydrogenase and promoting the protein expression of occludin. CNMs were further found to ameliorate inflammation via modulation of tumor factor α, toll-like receptor 4 and nuclear factor κB (NF-κB) expression via inhibition of mitogen-activated protein kinase and NF-κB activation and improved antioxidant activity. Taken together, CNMs could protect the host against <i>E. coli O157</i>-induced intestinal barrier damage and inflammation, showing that CNMs have great advantages and potential application as novel antimicrobial polymers in the food industry as food biopreservatives, bringing new hope for the treatment of bacterial infections.https://www.mdpi.com/1422-0067/22/24/13580chitosan nanoparticlesantimicrobial peptidemicrocin J25foodborne pathogens<i>E. coli O157</i>anti-inflammation |
| spellingShingle | Ming Kuang Haitao Yu Shiyan Qiao Tao Huang Jiaqi Zhang Mingchao Sun Xiumei Shi Han Chen A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory Responses International Journal of Molecular Sciences chitosan nanoparticles antimicrobial peptide microcin J25 foodborne pathogens <i>E. coli O157</i> anti-inflammation |
| title | A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory Responses |
| title_full | A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory Responses |
| title_fullStr | A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory Responses |
| title_full_unstemmed | A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory Responses |
| title_short | A Novel Nano-Antimicrobial Polymer Engineered with Chitosan Nanoparticles and Bioactive Peptides as Promising Food Biopreservative Effective against Foodborne Pathogen <i>E. coli O157</i>-Caused Epithelial Barrier Dysfunction and Inflammatory Responses |
| title_sort | novel nano antimicrobial polymer engineered with chitosan nanoparticles and bioactive peptides as promising food biopreservative effective against foodborne pathogen i e coli o157 i caused epithelial barrier dysfunction and inflammatory responses |
| topic | chitosan nanoparticles antimicrobial peptide microcin J25 foodborne pathogens <i>E. coli O157</i> anti-inflammation |
| url | https://www.mdpi.com/1422-0067/22/24/13580 |
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