Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings
Background Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the...
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PeerJ Inc.
2021-06-01
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| Online Access: | https://peerj.com/articles/11114.pdf |
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| author | Renata Duarte de Souza-Rodrigues Bruna Puty Laís Bonfim Lygia Sega Nogueira Priscila Cunha Nascimento Leonardo Oliveira Bittencourt Roberta Souza D’Almeida Couto Carlos Augusto Galvão Barboza Edivaldo Herculano Corrêa de Oliveira Marcia Martins Marques Rafael Rodrigues Lima |
| author_facet | Renata Duarte de Souza-Rodrigues Bruna Puty Laís Bonfim Lygia Sega Nogueira Priscila Cunha Nascimento Leonardo Oliveira Bittencourt Roberta Souza D’Almeida Couto Carlos Augusto Galvão Barboza Edivaldo Herculano Corrêa de Oliveira Marcia Martins Marques Rafael Rodrigues Lima |
| author_sort | Renata Duarte de Souza-Rodrigues |
| collection | DOAJ |
| description | Background Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure. Methods Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 µM of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 µM MeHg were tested by measuring glutathione (GSH) level. Results It was observed that cell viability decreased significantly after exposure to 2.5 and 5 µM of MeHg, but the metabolic viability only decreased significantly at 5 µM MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 µM has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells. |
| first_indexed | 2024-03-09T08:08:05Z |
| format | Article |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:08:05Z |
| publishDate | 2021-06-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-e4893cc2ddd94203bbc87edd7a8067012023-12-02T23:45:10ZengPeerJ Inc.PeerJ2167-83592021-06-019e1111410.7717/peerj.11114Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findingsRenata Duarte de Souza-Rodrigues0Bruna Puty1Laís Bonfim2Lygia Sega Nogueira3Priscila Cunha Nascimento4Leonardo Oliveira Bittencourt5Roberta Souza D’Almeida Couto6Carlos Augusto Galvão Barboza7Edivaldo Herculano Corrêa de Oliveira8Marcia Martins Marques9Rafael Rodrigues Lima10Institute of Arts Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, BrazilFaculty of Dentistry, Federal University of Pará (UFPA), Belém, Pará, BrazilDepartment of Morphology, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, BrazilLaboratory of Tissue Culture and Cytogenetics, Environment Section, Evandro Chagas Institute, Ananindeua, Pará, BrazilGraduation Program, School of Dentistry, Ibirapuera University (UNIb), São Paulo, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil, BrazilBackground Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure. Methods Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 µM of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 µM MeHg were tested by measuring glutathione (GSH) level. Results It was observed that cell viability decreased significantly after exposure to 2.5 and 5 µM of MeHg, but the metabolic viability only decreased significantly at 5 µM MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 µM has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells.https://peerj.com/articles/11114.pdfMethylmercury (MeHg)Dental pulp stem cellsCell viabilityCell metabolismOxidative stress |
| spellingShingle | Renata Duarte de Souza-Rodrigues Bruna Puty Laís Bonfim Lygia Sega Nogueira Priscila Cunha Nascimento Leonardo Oliveira Bittencourt Roberta Souza D’Almeida Couto Carlos Augusto Galvão Barboza Edivaldo Herculano Corrêa de Oliveira Marcia Martins Marques Rafael Rodrigues Lima Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings PeerJ Methylmercury (MeHg) Dental pulp stem cells Cell viability Cell metabolism Oxidative stress |
| title | Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
| title_full | Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
| title_fullStr | Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
| title_full_unstemmed | Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
| title_short | Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings |
| title_sort | methylmercury induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells the first toxicological findings |
| topic | Methylmercury (MeHg) Dental pulp stem cells Cell viability Cell metabolism Oxidative stress |
| url | https://peerj.com/articles/11114.pdf |
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