Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods
Leishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropri...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1632 |
| _version_ | 1797550126301970432 |
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| author | Juliana Quero Reimão Elizabeth Magiolo Coser Monica Ran Lee Adriano Cappellazzo Coelho |
| author_facet | Juliana Quero Reimão Elizabeth Magiolo Coser Monica Ran Lee Adriano Cappellazzo Coelho |
| author_sort | Juliana Quero Reimão |
| collection | DOAJ |
| description | Leishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropriate resources is still a problem. Most accessible methods for diagnosis, particularly in these areas, do not include the identification of each one of more than 20 species responsible for the disease. Here, we summarize the main methods used for the detection and identification of leishmaniasis that can be performed by demonstration of the parasite in biological samples from the patient through microscopic examination, by in vitro culture or animal inoculation; by molecular methods through the detection of parasite DNA; or by immunological methods through the detection of parasite antigens that may be present in urine or through the detection of specific antibodies against the parasite. Potential new methods that can be applied for laboratory diagnosis of leishmaniasis are also discussed. |
| first_indexed | 2024-03-10T15:25:05Z |
| format | Article |
| id | doaj.art-e48f0cb30cca4b8594a1f36173d41137 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:25:05Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e48f0cb30cca4b8594a1f36173d411372023-11-20T18:09:23ZengMDPI AGMicroorganisms2076-26072020-10-01811163210.3390/microorganisms8111632Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future MethodsJuliana Quero Reimão0Elizabeth Magiolo Coser1Monica Ran Lee2Adriano Cappellazzo Coelho3Departamento de Morfologia e Patologia Básica, Faculdade de Medicina de Jundiaí, Jundiaí 13202-550, BrazilDepartamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-862, BrazilDepartamento de Morfologia e Patologia Básica, Faculdade de Medicina de Jundiaí, Jundiaí 13202-550, BrazilDepartamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-862, BrazilLeishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropriate resources is still a problem. Most accessible methods for diagnosis, particularly in these areas, do not include the identification of each one of more than 20 species responsible for the disease. Here, we summarize the main methods used for the detection and identification of leishmaniasis that can be performed by demonstration of the parasite in biological samples from the patient through microscopic examination, by in vitro culture or animal inoculation; by molecular methods through the detection of parasite DNA; or by immunological methods through the detection of parasite antigens that may be present in urine or through the detection of specific antibodies against the parasite. Potential new methods that can be applied for laboratory diagnosis of leishmaniasis are also discussed.https://www.mdpi.com/2076-2607/8/11/1632leishmaniasiscutaneous leishmaniasisvisceral leishmaniasis<i>Leishmania</i>diagnosis |
| spellingShingle | Juliana Quero Reimão Elizabeth Magiolo Coser Monica Ran Lee Adriano Cappellazzo Coelho Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods Microorganisms leishmaniasis cutaneous leishmaniasis visceral leishmaniasis <i>Leishmania</i> diagnosis |
| title | Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods |
| title_full | Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods |
| title_fullStr | Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods |
| title_full_unstemmed | Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods |
| title_short | Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods |
| title_sort | laboratory diagnosis of cutaneous and visceral leishmaniasis current and future methods |
| topic | leishmaniasis cutaneous leishmaniasis visceral leishmaniasis <i>Leishmania</i> diagnosis |
| url | https://www.mdpi.com/2076-2607/8/11/1632 |
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