Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage
Ensiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and Pediococcus pentosaceus on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1035942/full |
| _version_ | 1828140285335437312 |
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| author | Xueyan Bao Haoran Feng Gang Guo Wenjie Huo Qinghong Li Qingfang Xu Qiang Liu Cong Wang Lei Chen |
| author_facet | Xueyan Bao Haoran Feng Gang Guo Wenjie Huo Qinghong Li Qingfang Xu Qiang Liu Cong Wang Lei Chen |
| author_sort | Xueyan Bao |
| collection | DOAJ |
| description | Ensiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and Pediococcus pentosaceus on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa. The application of laccase and Pediococcus pentosaceus combination was more potent in modulating the fermentation quality of silage than laccase and Pediococcus pentosaceus alone, as indicated by higher lactic acid contents and lactic acid to acetic acid ratios, and lower pH, dry matter losses, and ammonia nitrogen contents. Moreover, treatments with additive enhanced protein preservation and structural carbohydrate degradation, while increasing true protein and water-soluble carbohydrate contents. By promoting lignin degradation, treatments containing laccase further facilitated the release of sugars from cellulose compared with treatment with Pediococcus pentosaceus alone. The additive treatments reduced the bacterial diversity and optimized the bacterial community composition of silage, with an increase in the relative abundance of desirable Lactobacillus and a decrease in the relative abundance of undesirable Enterobacter and Klebsiella. PICRUSt functional prediction based on Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed that PL and LPL treatments increased the metabolism of membrane transport, carbohydrate, and terpenoids and polyketides related to fermentation activities. It can be concluded that bioaugmented ensiling with laccase and Pediococcus pentosaceus combination can be an effective and practical strategy to improve silage fermentation and nutrient preservation of alfalfa silage. |
| first_indexed | 2024-04-11T19:06:48Z |
| format | Article |
| id | doaj.art-e493549cc62a48fd9770eb1e9bbadf30 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T19:06:48Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e493549cc62a48fd9770eb1e9bbadf302022-12-22T04:07:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10359421035942Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silageXueyan Bao0Haoran Feng1Gang Guo2Wenjie Huo3Qinghong Li4Qingfang Xu5Qiang Liu6Cong Wang7Lei Chen8College of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaEnsiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and Pediococcus pentosaceus on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa. The application of laccase and Pediococcus pentosaceus combination was more potent in modulating the fermentation quality of silage than laccase and Pediococcus pentosaceus alone, as indicated by higher lactic acid contents and lactic acid to acetic acid ratios, and lower pH, dry matter losses, and ammonia nitrogen contents. Moreover, treatments with additive enhanced protein preservation and structural carbohydrate degradation, while increasing true protein and water-soluble carbohydrate contents. By promoting lignin degradation, treatments containing laccase further facilitated the release of sugars from cellulose compared with treatment with Pediococcus pentosaceus alone. The additive treatments reduced the bacterial diversity and optimized the bacterial community composition of silage, with an increase in the relative abundance of desirable Lactobacillus and a decrease in the relative abundance of undesirable Enterobacter and Klebsiella. PICRUSt functional prediction based on Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed that PL and LPL treatments increased the metabolism of membrane transport, carbohydrate, and terpenoids and polyketides related to fermentation activities. It can be concluded that bioaugmented ensiling with laccase and Pediococcus pentosaceus combination can be an effective and practical strategy to improve silage fermentation and nutrient preservation of alfalfa silage.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1035942/fullalfalfa silagebio-additivesbacterial communityfermentation qualityenzymatic hydrolysis |
| spellingShingle | Xueyan Bao Haoran Feng Gang Guo Wenjie Huo Qinghong Li Qingfang Xu Qiang Liu Cong Wang Lei Chen Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage Frontiers in Microbiology alfalfa silage bio-additives bacterial community fermentation quality enzymatic hydrolysis |
| title | Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage |
| title_full | Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage |
| title_fullStr | Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage |
| title_full_unstemmed | Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage |
| title_short | Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage |
| title_sort | effects of laccase and lactic acid bacteria on the fermentation quality nutrient composition enzymatic hydrolysis and bacterial community of alfalfa silage |
| topic | alfalfa silage bio-additives bacterial community fermentation quality enzymatic hydrolysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1035942/full |
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