Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear Memory
Rac1, a member of the Rho family of small GTPases, is crucial for morphological changes of the mature neuronal synapse including spine formation and activity-dependent spine enlargement, while its role in the formation of associated memories, such as conditioned fear memory, is not clear. Here we re...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-11-01
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| Series: | Frontiers in Molecular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnmol.2015.00065/full |
| _version_ | 1818548691423723520 |
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| author | Qinqin eGao Wenqing eYao Junjun eWang Tong eYang Cao eLiu Yezheng eTao Yuejun eChen Xing eLiu Lan eMa |
| author_facet | Qinqin eGao Wenqing eYao Junjun eWang Tong eYang Cao eLiu Yezheng eTao Yuejun eChen Xing eLiu Lan eMa |
| author_sort | Qinqin eGao |
| collection | DOAJ |
| description | Rac1, a member of the Rho family of small GTPases, is crucial for morphological changes of the mature neuronal synapse including spine formation and activity-dependent spine enlargement, while its role in the formation of associated memories, such as conditioned fear memory, is not clear. Here we report that selective deletion of Rac1 in excitatory neurons, but not in parvalbumin inhibitory neurons, impaired short- and long-term memories (STM and LTM) of fear conditioning. Conditional knockout of Rac1 before associative fear training in the basolateral amygdala (BLA), a key area for fear memory acquisition and storage, impaired fear memory. The expression of dominant-negative mutant of Rac1, or infusion of Rac1 inhibitor NSC23766 into BLA blocked both STM and LTM of fear conditioning. Furthermore, selective inhibition of Rac1 activation in BLA immediately following fear conditioning impaired STM, demonstrating that fear conditioning-induced Rac1 activation in BLA plays a critical role in the formation of both STM and LTM of conditioned fear. |
| first_indexed | 2024-12-12T08:23:34Z |
| format | Article |
| id | doaj.art-e493cfc44f5d4f00b5463e44850c24b5 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-12T08:23:34Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-e493cfc44f5d4f00b5463e44850c24b52022-12-22T00:31:20ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992015-11-01810.3389/fnmol.2015.00065166248Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear MemoryQinqin eGao0Wenqing eYao1Junjun eWang2Tong eYang3Cao eLiu4Yezheng eTao5Yuejun eChen6Xing eLiu7Lan eMa8Fudan UniversityFudan UniversityFudan UniversityFudan UniversityFudan UniversityFudan UniversityFudan UniversityFudan UniversityFudan UniversityRac1, a member of the Rho family of small GTPases, is crucial for morphological changes of the mature neuronal synapse including spine formation and activity-dependent spine enlargement, while its role in the formation of associated memories, such as conditioned fear memory, is not clear. Here we report that selective deletion of Rac1 in excitatory neurons, but not in parvalbumin inhibitory neurons, impaired short- and long-term memories (STM and LTM) of fear conditioning. Conditional knockout of Rac1 before associative fear training in the basolateral amygdala (BLA), a key area for fear memory acquisition and storage, impaired fear memory. The expression of dominant-negative mutant of Rac1, or infusion of Rac1 inhibitor NSC23766 into BLA blocked both STM and LTM of fear conditioning. Furthermore, selective inhibition of Rac1 activation in BLA immediately following fear conditioning impaired STM, demonstrating that fear conditioning-induced Rac1 activation in BLA plays a critical role in the formation of both STM and LTM of conditioned fear.http://journal.frontiersin.org/Journal/10.3389/fnmol.2015.00065/fullFear conditioningRac1blaLTMmemory formationSTM |
| spellingShingle | Qinqin eGao Wenqing eYao Junjun eWang Tong eYang Cao eLiu Yezheng eTao Yuejun eChen Xing eLiu Lan eMa Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear Memory Frontiers in Molecular Neuroscience Fear conditioning Rac1 bla LTM memory formation STM |
| title | Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear Memory |
| title_full | Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear Memory |
| title_fullStr | Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear Memory |
| title_full_unstemmed | Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear Memory |
| title_short | Post-training Activation of Rac1 in the Basolateral Amygdala Is Required for the Formation of both Short-term and Long-term Auditory Fear Memory |
| title_sort | post training activation of rac1 in the basolateral amygdala is required for the formation of both short term and long term auditory fear memory |
| topic | Fear conditioning Rac1 bla LTM memory formation STM |
| url | http://journal.frontiersin.org/Journal/10.3389/fnmol.2015.00065/full |
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