<i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides

<i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides

Whole genome sequences of two <i>Acinetobacter baumannii</i> clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are relate...

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Bibliographic Details
Main Authors: Anastasiya A. Kasimova, Nikolay P. Arbatsky, Jacob Tickner, Johanna J. Kenyon, Ruth M. Hall, Michael M. Shneider, Alina A. Dzhaparova, Alexander S. Shashkov, Alexander O. Chizhov, Anastasiya V. Popova, Yuriy A. Knirel
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Acinetobacter baumannii</i>
capsular polysaccharide
K locus
K106
K112
6-deoxy-<span style="font-variant: small-caps">l</span>-talose
Online Access:https://www.mdpi.com/1422-0067/22/11/5641
Description
Summary:Whole genome sequences of two <i>Acinetobacter baumannii</i> clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of <span style="font-variant: small-caps;">l</span>-rhamnose (<span style="font-variant: small-caps;">l</span>-Rha<i>p</i>) and 6-deoxy-<span style="font-variant: small-caps;">l</span>-talose (<span style="font-variant: small-caps;">l</span>-6dTal<i>p</i>). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) <span style="font-variant: small-caps;">l</span>-6dTal<i>p</i>-(1→3)-D-Glc<i>p</i>NAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α <span style="font-variant: small-caps;">l</span>-Rha<i>p</i> side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different <i>wzy</i> gene is found in KL112. However, though both KL106 and KL112 share the <i>atr8</i> acetyltransferase gene with KL83, only K83 is acetylated.
ISSN:1661-6596
1422-0067

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