<i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides
Whole genome sequences of two <i>Acinetobacter baumannii</i> clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are relate...
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2021-05-01
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| author | Anastasiya A. Kasimova Nikolay P. Arbatsky Jacob Tickner Johanna J. Kenyon Ruth M. Hall Michael M. Shneider Alina A. Dzhaparova Alexander S. Shashkov Alexander O. Chizhov Anastasiya V. Popova Yuriy A. Knirel |
| author_facet | Anastasiya A. Kasimova Nikolay P. Arbatsky Jacob Tickner Johanna J. Kenyon Ruth M. Hall Michael M. Shneider Alina A. Dzhaparova Alexander S. Shashkov Alexander O. Chizhov Anastasiya V. Popova Yuriy A. Knirel |
| author_sort | Anastasiya A. Kasimova |
| collection | DOAJ |
| description | Whole genome sequences of two <i>Acinetobacter baumannii</i> clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of <span style="font-variant: small-caps;">l</span>-rhamnose (<span style="font-variant: small-caps;">l</span>-Rha<i>p</i>) and 6-deoxy-<span style="font-variant: small-caps;">l</span>-talose (<span style="font-variant: small-caps;">l</span>-6dTal<i>p</i>). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) <span style="font-variant: small-caps;">l</span>-6dTal<i>p</i>-(1→3)-D-Glc<i>p</i>NAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α <span style="font-variant: small-caps;">l</span>-Rha<i>p</i> side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different <i>wzy</i> gene is found in KL112. However, though both KL106 and KL112 share the <i>atr8</i> acetyltransferase gene with KL83, only K83 is acetylated. |
| first_indexed | 2024-03-10T11:02:55Z |
| format | Article |
| id | doaj.art-e495c160de7c455a8e2189c13c09cc41 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:02:55Z |
| publishDate | 2021-05-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e495c160de7c455a8e2189c13c09cc412023-11-21T21:24:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012211564110.3390/ijms22115641<i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular PolysaccharidesAnastasiya A. Kasimova0Nikolay P. Arbatsky1Jacob Tickner2Johanna J. Kenyon3Ruth M. Hall4Michael M. Shneider5Alina A. Dzhaparova6Alexander S. Shashkov7Alexander O. Chizhov8Anastasiya V. Popova9Yuriy A. Knirel10N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaCentre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4059, AustraliaCentre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4059, AustraliaSchool of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, AustraliaM. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaState Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279 Moscow Region, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaWhole genome sequences of two <i>Acinetobacter baumannii</i> clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of <span style="font-variant: small-caps;">l</span>-rhamnose (<span style="font-variant: small-caps;">l</span>-Rha<i>p</i>) and 6-deoxy-<span style="font-variant: small-caps;">l</span>-talose (<span style="font-variant: small-caps;">l</span>-6dTal<i>p</i>). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) <span style="font-variant: small-caps;">l</span>-6dTal<i>p</i>-(1→3)-D-Glc<i>p</i>NAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α <span style="font-variant: small-caps;">l</span>-Rha<i>p</i> side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different <i>wzy</i> gene is found in KL112. However, though both KL106 and KL112 share the <i>atr8</i> acetyltransferase gene with KL83, only K83 is acetylated.https://www.mdpi.com/1422-0067/22/11/5641<i>Acinetobacter baumannii</i>capsular polysaccharideK locusK106K1126-deoxy-<span style="font-variant: small-caps">l</span>-talose |
| spellingShingle | Anastasiya A. Kasimova Nikolay P. Arbatsky Jacob Tickner Johanna J. Kenyon Ruth M. Hall Michael M. Shneider Alina A. Dzhaparova Alexander S. Shashkov Alexander O. Chizhov Anastasiya V. Popova Yuriy A. Knirel <i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides International Journal of Molecular Sciences <i>Acinetobacter baumannii</i> capsular polysaccharide K locus K106 K112 6-deoxy-<span style="font-variant: small-caps">l</span>-talose |
| title | <i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides |
| title_full | <i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides |
| title_fullStr | <i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides |
| title_full_unstemmed | <i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides |
| title_short | <i>Acinetobacter baumannii</i> K106 and K112: Two Structurally and Genetically Related 6-Deoxy-<span style="font-variant: small-caps">l</span>-talose-Containing Capsular Polysaccharides |
| title_sort | i acinetobacter baumannii i k106 and k112 two structurally and genetically related 6 deoxy span style font variant small caps l span talose containing capsular polysaccharides |
| topic | <i>Acinetobacter baumannii</i> capsular polysaccharide K locus K106 K112 6-deoxy-<span style="font-variant: small-caps">l</span>-talose |
| url | https://www.mdpi.com/1422-0067/22/11/5641 |
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