Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering
Abalone shells, which contain both organic and inorganic matter, can facilitate bone remodeling and have been used to fabricate three-dimensional (3D)-printed scaffolds for bone regeneration. Herein, polycaprolactone (PCL) scaffolds were fabricated using 3D printing with abalone shell particles (ASP...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127521007838 |
| _version_ | 1828923491905175552 |
|---|---|
| author | Dahong Kim Jihye Lee Ji Min Seok Joo-Yun Jung Jun Hee Lee Jun Sik Lee Kangwon Lee Su A Park |
| author_facet | Dahong Kim Jihye Lee Ji Min Seok Joo-Yun Jung Jun Hee Lee Jun Sik Lee Kangwon Lee Su A Park |
| author_sort | Dahong Kim |
| collection | DOAJ |
| description | Abalone shells, which contain both organic and inorganic matter, can facilitate bone remodeling and have been used to fabricate three-dimensional (3D)-printed scaffolds for bone regeneration. Herein, polycaprolactone (PCL) scaffolds were fabricated using 3D printing with abalone shell particles (ASPs) used in high-temperature processing. ASPs were heated to approximately the melting point of PCL and thermally embedded in 3D-printed PCL using a relatively simple process. The morphology and roughness of the composite scaffold changed according to the weight of ASPs used. The scaffolds were grouped as follows: ASP25 (25 mg), ASP50 (50 mg), and ASP100 (100 mg). The ASP25 group exhibited optimum cell viability and proliferation because of the direct influence of roughness and rapid pH changes. The ASP25 and ASP100 groups showed the highest alkaline phosphatase activity. This could be attributed to the effect of the alkaline environment, dissolution of calcium ions, and presence of bioactive molecules in the ASPs that could support bone regeneration. Therefore, the ASP25 group was the most suitable for fabricating bone scaffolds. This study revealed the potential applicability of ASP-embedded scaffolds in bone tissue engineering involving natural bio-organisms that self-mineralize in a process similar to human bone formation. |
| first_indexed | 2024-12-13T22:40:24Z |
| format | Article |
| id | doaj.art-e49c32750a1e4e31bd819a5f265a955b |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-13T22:40:24Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-e49c32750a1e4e31bd819a5f265a955b2022-12-21T23:28:53ZengElsevierMaterials & Design0264-12752021-12-01212110228Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineeringDahong Kim0Jihye Lee1Ji Min Seok2Joo-Yun Jung3Jun Hee Lee4Jun Sik Lee5Kangwon Lee6Su A Park7Nano Convergence & Manufacturing Systems, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of Korea; Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of KoreaNano Convergence & Manufacturing Systems, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of KoreaNano Convergence & Manufacturing Systems, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of Korea; Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of KoreaNano Convergence & Manufacturing Systems, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of KoreaNano Convergence & Manufacturing Systems, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of KoreaDepartment of Life Science, Immunology Research Lab, College of Natural Sciences, Chosun University, Gwangju 61452, Republic of KoreaDepartment of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea; Corresponding authors at: Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea (Kangwon Lee) and Department of Nature-Inspired Nano Convergence Systems, Nano Convergence & Manufacturing Systems Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of Korea (Su A Park).Nano Convergence & Manufacturing Systems, Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of Korea; Corresponding authors at: Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea (Kangwon Lee) and Department of Nature-Inspired Nano Convergence Systems, Nano Convergence & Manufacturing Systems Korea Institute of Machinery and Materials (KIMM), Daejeon 34103, Republic of Korea (Su A Park).Abalone shells, which contain both organic and inorganic matter, can facilitate bone remodeling and have been used to fabricate three-dimensional (3D)-printed scaffolds for bone regeneration. Herein, polycaprolactone (PCL) scaffolds were fabricated using 3D printing with abalone shell particles (ASPs) used in high-temperature processing. ASPs were heated to approximately the melting point of PCL and thermally embedded in 3D-printed PCL using a relatively simple process. The morphology and roughness of the composite scaffold changed according to the weight of ASPs used. The scaffolds were grouped as follows: ASP25 (25 mg), ASP50 (50 mg), and ASP100 (100 mg). The ASP25 group exhibited optimum cell viability and proliferation because of the direct influence of roughness and rapid pH changes. The ASP25 and ASP100 groups showed the highest alkaline phosphatase activity. This could be attributed to the effect of the alkaline environment, dissolution of calcium ions, and presence of bioactive molecules in the ASPs that could support bone regeneration. Therefore, the ASP25 group was the most suitable for fabricating bone scaffolds. This study revealed the potential applicability of ASP-embedded scaffolds in bone tissue engineering involving natural bio-organisms that self-mineralize in a process similar to human bone formation.http://www.sciencedirect.com/science/article/pii/S0264127521007838Three-dimensional printingAbalone shellBone tissue engineeringBone scaffoldBioactive scaffoldMarine organism |
| spellingShingle | Dahong Kim Jihye Lee Ji Min Seok Joo-Yun Jung Jun Hee Lee Jun Sik Lee Kangwon Lee Su A Park Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering Materials & Design Three-dimensional printing Abalone shell Bone tissue engineering Bone scaffold Bioactive scaffold Marine organism |
| title | Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering |
| title_full | Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering |
| title_fullStr | Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering |
| title_full_unstemmed | Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering |
| title_short | Three-dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering |
| title_sort | three dimensional bioprinting of bioactive scaffolds with thermally embedded abalone shell particles for bone tissue engineering |
| topic | Three-dimensional printing Abalone shell Bone tissue engineering Bone scaffold Bioactive scaffold Marine organism |
| url | http://www.sciencedirect.com/science/article/pii/S0264127521007838 |
| work_keys_str_mv | AT dahongkim threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering AT jihyelee threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering AT jiminseok threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering AT jooyunjung threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering AT junheelee threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering AT junsiklee threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering AT kangwonlee threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering AT suapark threedimensionalbioprintingofbioactivescaffoldswiththermallyembeddedabaloneshellparticlesforbonetissueengineering |