| Summary: | <i>Background:</i> The spread of carbapenemase genes, such as <i>bla</i><sub>NDM-1</sub>, in <i>Proteus mirabilis</i> poses a public health threat. The aim of the study was to characterize the genome and plasmids sequences of an NDM-1-positive strain (IBCRE14), which was isolated in 2019 from a catheterized patient hospitalized in Italy. <i>Methods</i>: Whole genome sequencing (WGS) of IBCRE14 was performed on extracted genomic DNA using Sequel I platform. Genome assembly was performed using “Microbial Assembly”. Genomic analysis was conducted by uploading the contigs to ResFinder and PlasmidFinder databases from the Center for Genomic Epidemiology. <i>Results:</i> IBCRE14 had a genome size of 4,018,329 bp and harboured genes coding for resistance to aminoglycosides (<i>aadA1</i>), phenicol (<i>cat</i>), tetracycline (<i>tetJ</i>), and trimethoprim (<i>dfrA1</i>). A large plasmid (pIB_NDM_1) harboured antibiotic resistance genes against sulphonamide (<i>sul1</i>), trimethoprim (<i>dfrA14</i>), tetracycline (<i>tetB</i>), rifampicin (<i>arr-2</i>), aminoglycosides (<i>aadA1, aph3-VI</i>), and beta-lactams (<i>bla</i><sub>OXA-10</sub>, <i>bla</i><sub>NDM-1</sub>). Furthermore, a small plasmid (pIB_COL3M) harboured a <i>qnrD1 </i>gene coding for quinolone resistance. <i>Conclusion:</i> The ability to conjugate and the presence of a composite antibiotic resistance island suggests that pIB_NDM_1 could both acquire more resistance genes and easily disseminate. To our knowledge, this is the first report on an untypable plasmid harbouring <i>bla</i><sub>NDM-1 </sub>in <i>P. mirabilis, </i>in Italy.
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