In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems
In the present study, a peptide possessing antioxidant properties was isolated from Nile tilapia (Oreochromis niloticus) scale gelatin. Gelatin protein was hydrolyzed using alcalase, pronase E, trypsin and pepsin. Antioxidant efficacy of respective hydrolysates were evaluated using 1,1-diphenyl-2-pi...
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| Format: | Article |
| Language: | English |
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Elsevier
2010-04-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646461000006X |
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| author | Dai-Hung Ngo Zhong-Ji Qian BoMi Ryu Jae W. Park Se-Kwon Kim |
| author_facet | Dai-Hung Ngo Zhong-Ji Qian BoMi Ryu Jae W. Park Se-Kwon Kim |
| author_sort | Dai-Hung Ngo |
| collection | DOAJ |
| description | In the present study, a peptide possessing antioxidant properties was isolated from Nile tilapia (Oreochromis niloticus) scale gelatin. Gelatin protein was hydrolyzed using alcalase, pronase E, trypsin and pepsin. Antioxidant efficacy of respective hydrolysates were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical anion scavenging activities. Moreover, protective effect on DNA damage caused by hydroxyl radicals generated was determined. Further, the level of reactive oxygen species (ROS) was determined using a fluorescence probe, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on mouse macrophages, RAW 264.7 cells. Among hydrolysates, alcalase-derived hydrolysate exhibited the highest antioxidant activity compared to other enzymatic hydrolysates. Therefore, it was further analyzed and the sequence of an active peptide present in it was identified as Asp-Pro-Ala-Leu-Ala-Thr-Glu-Pro-Asp-Pro-Met-Pro-Phe (1382.57 Da). This peptide showed no cytotoxic effect on mouse macrophages (RAW 264.7) and human lung fibroblasts (MRC-5). In addition, it scavenged hydroxyl, DPPH and superoxide radicals at the IC50 values of 7.56, 8.82 and 17.83 μM, respectively. These results suggest that the peptide derived from Nile tilapia (O. niloticus) scale gelatin acts as a candidate against oxidative stress and could be used as a potential functional food ingredient. |
| first_indexed | 2024-12-21T13:05:25Z |
| format | Article |
| id | doaj.art-e4a01ba7ecda45e5b45c8592fcafebac |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T13:05:25Z |
| publishDate | 2010-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e4a01ba7ecda45e5b45c8592fcafebac2022-12-21T19:03:03ZengElsevierJournal of Functional Foods1756-46462010-04-0122107117In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systemsDai-Hung Ngo0Zhong-Ji Qian1BoMi Ryu2Jae W. Park3Se-Kwon Kim4Department of Chemistry, Pukyong National University, Busan 608-737, Republic of KoreaMarine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of KoreaDepartment of Chemistry, Pukyong National University, Busan 608-737, Republic of KoreaFood Science and Technology, Seafood Research and Education Center, 2001 Marine Dr., Rm 253 Astoria, OR 97103, USADepartment of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea; Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea; Corresponding author: Address: Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea. Tel.: +82 51 629 7094; fax: +82 51 629 7099.In the present study, a peptide possessing antioxidant properties was isolated from Nile tilapia (Oreochromis niloticus) scale gelatin. Gelatin protein was hydrolyzed using alcalase, pronase E, trypsin and pepsin. Antioxidant efficacy of respective hydrolysates were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical anion scavenging activities. Moreover, protective effect on DNA damage caused by hydroxyl radicals generated was determined. Further, the level of reactive oxygen species (ROS) was determined using a fluorescence probe, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on mouse macrophages, RAW 264.7 cells. Among hydrolysates, alcalase-derived hydrolysate exhibited the highest antioxidant activity compared to other enzymatic hydrolysates. Therefore, it was further analyzed and the sequence of an active peptide present in it was identified as Asp-Pro-Ala-Leu-Ala-Thr-Glu-Pro-Asp-Pro-Met-Pro-Phe (1382.57 Da). This peptide showed no cytotoxic effect on mouse macrophages (RAW 264.7) and human lung fibroblasts (MRC-5). In addition, it scavenged hydroxyl, DPPH and superoxide radicals at the IC50 values of 7.56, 8.82 and 17.83 μM, respectively. These results suggest that the peptide derived from Nile tilapia (O. niloticus) scale gelatin acts as a candidate against oxidative stress and could be used as a potential functional food ingredient.http://www.sciencedirect.com/science/article/pii/S175646461000006XNile tilapia scaleGelatin hydrolysatesFree radical scavengingAntioxidative peptideElectron spin resonanceRAW 264.7 cells |
| spellingShingle | Dai-Hung Ngo Zhong-Ji Qian BoMi Ryu Jae W. Park Se-Kwon Kim In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems Journal of Functional Foods Nile tilapia scale Gelatin hydrolysates Free radical scavenging Antioxidative peptide Electron spin resonance RAW 264.7 cells |
| title | In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems |
| title_full | In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems |
| title_fullStr | In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems |
| title_full_unstemmed | In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems |
| title_short | In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems |
| title_sort | in vitro antioxidant activity of a peptide isolated from nile tilapia oreochromis niloticus scale gelatin in free radical mediated oxidative systems |
| topic | Nile tilapia scale Gelatin hydrolysates Free radical scavenging Antioxidative peptide Electron spin resonance RAW 264.7 cells |
| url | http://www.sciencedirect.com/science/article/pii/S175646461000006X |
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