Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema
Objectives: Talaromyces purpurogenus is a fungus species widely found in the marine environment. When in a hostile environment, it produces a series of secondary metabolites with important biotechnological potential, such as meroterpenoids. In our previous report, we demonstrated that the culture br...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722002026 |
| _version_ | 1818552152886345728 |
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| author | Amanda Mara Teles Gabriel Silva Xavier Wendel Fragoso de Freitas Moreira Carla Junqueira Moragas-Tellis Maria do Socorro dos Santos Chagas Maria Dutra Behrens Ana Lúcia Abreu-Silva Geusa Felipa de Barros Bezerra Kátia da Silva Calabrese Maria do Desterro Soares Brandão Nascimento Fernando Almeida-Souza |
| author_facet | Amanda Mara Teles Gabriel Silva Xavier Wendel Fragoso de Freitas Moreira Carla Junqueira Moragas-Tellis Maria do Socorro dos Santos Chagas Maria Dutra Behrens Ana Lúcia Abreu-Silva Geusa Felipa de Barros Bezerra Kátia da Silva Calabrese Maria do Desterro Soares Brandão Nascimento Fernando Almeida-Souza |
| author_sort | Amanda Mara Teles |
| collection | DOAJ |
| description | Objectives: Talaromyces purpurogenus is a fungus species widely found in the marine environment. When in a hostile environment, it produces a series of secondary metabolites with important biotechnological potential, such as meroterpenoids. In our previous report, we demonstrated that the culture broth ethyl acetate extract from T. purpurogenus reduced the cellular infiltration of lymphoid organs and circulating levels of TNF-α. To elucidate this anti-inflammatory effect, this work aimed to study T. purpurogenus isolated from a marine-polluted environment regarding the chemical composition of its culture broth and mycelial ethyl acetate extract (CBEAE and MEAE respectively), as well as its action on pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and carrageenan-induced mice paw edema. Methods: Chemical characterization was performed by HPLC-DAD-UV. Cytotoxicity in vitro and endotoxin quantification in extracts were preformed prior the experiments. In vitro inhibitory effect of T. purpurogenus extracts were evaluated by cytokines and nitrite quantification in cell supernatant of RAW 264.7 cells. The effect of T. purpurogenus in murine acute inflammation model of carrageenan-induced paw edema was verified by thickness kinetic and histology evaluation. Results: The CBEAE demonstrated a richer chemical composition than MEAE, presenting nine meroterpenoid compounds, while MEAE displayed only two meroterpenoid and one phenolic compound. Cytotoxicity in RAW 264.7 macrophages was not observed at any concentration treatment for both extracts. Endotoxin quantification also demonstrated absence of endotoxins in both extracts. At 500 μg/mL, the CBEAE and MEAE extracts inhibited nitrite (p = 0.0450 and p = 0.0497), TNF-α (p = 0.0076 and p = 0.0001) and IL-1β (p = 0.0042 and p = 0.0031), but only CBEAE inhibited IL-6 levels in LPS-stimulated cells (p = 0.0234). CBEAE, but not MEAE, also inhibited nitrite (p = 0.0353) and IL-1β (p = 0.0386) in non-stimulated cells. In addition, CBEAE at 250 and 500 mg/kg inhibited edema thickness and mast cells (p = 0.0303 and p = 0.0130) in λ-carrageenan-induced mice paw edema. Conclusions: Talaromyces purpurogenus isolated from marine-polluted environment inhibited pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in murine model of paw edema. The promising anti-inflammatory activity in vitro and in vivo, especially the culture broth ethyl acetate extract, demonstrates its potential for further studies to elucidate its mechanism of action. |
| first_indexed | 2024-12-12T09:09:28Z |
| format | Article |
| id | doaj.art-e4a18694290e471a9aff4566ecc53bbe |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-12T09:09:28Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-e4a18694290e471a9aff4566ecc53bbe2022-12-22T00:29:34ZengElsevierJournal of King Saud University: Science1018-36472022-06-01344102021Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edemaAmanda Mara Teles0Gabriel Silva Xavier1Wendel Fragoso de Freitas Moreira2Carla Junqueira Moragas-Tellis3Maria do Socorro dos Santos Chagas4Maria Dutra Behrens5Ana Lúcia Abreu-Silva6Geusa Felipa de Barros Bezerra7Kátia da Silva Calabrese8Maria do Desterro Soares Brandão Nascimento9Fernando Almeida-Souza10Pós-Graduação em Saúde do Adulto, Universidade Federal do Maranhão, São Luís 65080-805, BrazilRede Nordeste de Biotecnologia, Universidade Federal do Maranhão, São Luís 65080-805, BrazilUniversidade Estadual do Maranhão, São Luís 65055-310, BrazilLaboratório de Produtos Naturais para Saúde Pública (LPNSP), Instituto de Tecnologia em Fármacos—Farmanguinhos, Fiocruz, Rio de Janeiro 21041-000, BrazilLaboratório de Produtos Naturais para Saúde Pública (LPNSP), Instituto de Tecnologia em Fármacos—Farmanguinhos, Fiocruz, Rio de Janeiro 21041-000, BrazilLaboratório de Produtos Naturais para Saúde Pública (LPNSP), Instituto de Tecnologia em Fármacos—Farmanguinhos, Fiocruz, Rio de Janeiro 21041-000, BrazilUniversidade Estadual do Maranhão, São Luís 65055-310, BrazilPós-Graduação em Saúde do Adulto, Universidade Federal do Maranhão, São Luís 65080-805, BrazilLaboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil; Corresponding author at: Laboratório de Imunomodulação e Protozoologia, Pavilhão Carlos Chagas, Sala 213, Manguinhos, Rio de Janeiro 21040-360, Brazil.Pós-Graduação em Saúde do Adulto, Universidade Federal do Maranhão, São Luís 65080-805, Brazil; Rede Nordeste de Biotecnologia, Universidade Federal do Maranhão, São Luís 65080-805, BrazilUniversidade Estadual do Maranhão, São Luís 65055-310, Brazil; Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilObjectives: Talaromyces purpurogenus is a fungus species widely found in the marine environment. When in a hostile environment, it produces a series of secondary metabolites with important biotechnological potential, such as meroterpenoids. In our previous report, we demonstrated that the culture broth ethyl acetate extract from T. purpurogenus reduced the cellular infiltration of lymphoid organs and circulating levels of TNF-α. To elucidate this anti-inflammatory effect, this work aimed to study T. purpurogenus isolated from a marine-polluted environment regarding the chemical composition of its culture broth and mycelial ethyl acetate extract (CBEAE and MEAE respectively), as well as its action on pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and carrageenan-induced mice paw edema. Methods: Chemical characterization was performed by HPLC-DAD-UV. Cytotoxicity in vitro and endotoxin quantification in extracts were preformed prior the experiments. In vitro inhibitory effect of T. purpurogenus extracts were evaluated by cytokines and nitrite quantification in cell supernatant of RAW 264.7 cells. The effect of T. purpurogenus in murine acute inflammation model of carrageenan-induced paw edema was verified by thickness kinetic and histology evaluation. Results: The CBEAE demonstrated a richer chemical composition than MEAE, presenting nine meroterpenoid compounds, while MEAE displayed only two meroterpenoid and one phenolic compound. Cytotoxicity in RAW 264.7 macrophages was not observed at any concentration treatment for both extracts. Endotoxin quantification also demonstrated absence of endotoxins in both extracts. At 500 μg/mL, the CBEAE and MEAE extracts inhibited nitrite (p = 0.0450 and p = 0.0497), TNF-α (p = 0.0076 and p = 0.0001) and IL-1β (p = 0.0042 and p = 0.0031), but only CBEAE inhibited IL-6 levels in LPS-stimulated cells (p = 0.0234). CBEAE, but not MEAE, also inhibited nitrite (p = 0.0353) and IL-1β (p = 0.0386) in non-stimulated cells. In addition, CBEAE at 250 and 500 mg/kg inhibited edema thickness and mast cells (p = 0.0303 and p = 0.0130) in λ-carrageenan-induced mice paw edema. Conclusions: Talaromyces purpurogenus isolated from marine-polluted environment inhibited pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in murine model of paw edema. The promising anti-inflammatory activity in vitro and in vivo, especially the culture broth ethyl acetate extract, demonstrates its potential for further studies to elucidate its mechanism of action.http://www.sciencedirect.com/science/article/pii/S1018364722002026MeroterpenoidFungusCytokineInflammationNitric oxideMast cells |
| spellingShingle | Amanda Mara Teles Gabriel Silva Xavier Wendel Fragoso de Freitas Moreira Carla Junqueira Moragas-Tellis Maria do Socorro dos Santos Chagas Maria Dutra Behrens Ana Lúcia Abreu-Silva Geusa Felipa de Barros Bezerra Kátia da Silva Calabrese Maria do Desterro Soares Brandão Nascimento Fernando Almeida-Souza Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema Journal of King Saud University: Science Meroterpenoid Fungus Cytokine Inflammation Nitric oxide Mast cells |
| title | Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema |
| title_full | Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema |
| title_fullStr | Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema |
| title_full_unstemmed | Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema |
| title_short | Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema |
| title_sort | talaromyces purpurogenus from a marine polluted environment inhibits pro inflammatory markers in lps stimulated raw 264 7 cells and in carrageenan induced paw edema |
| topic | Meroterpenoid Fungus Cytokine Inflammation Nitric oxide Mast cells |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722002026 |
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