Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts
Type 2 diabetes mellitus (T2DM) emerged as a major health care concern in modern society, primarily due to lifestyle changes and dietary habits. Obesity-induced insulin resistance is considered as the major pathogenic factor in T2DM. In this study, we investigated the effect of vindoline, an indole...
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MDPI AG
2023-06-01
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| author | Beegum Noorjahan Shijina Achuthan Radhika Sainulabdeen Sherin Prabath Gopalakrishnan Biju |
| author_facet | Beegum Noorjahan Shijina Achuthan Radhika Sainulabdeen Sherin Prabath Gopalakrishnan Biju |
| author_sort | Beegum Noorjahan Shijina |
| collection | DOAJ |
| description | Type 2 diabetes mellitus (T2DM) emerged as a major health care concern in modern society, primarily due to lifestyle changes and dietary habits. Obesity-induced insulin resistance is considered as the major pathogenic factor in T2DM. In this study, we investigated the effect of vindoline, an indole alkaloid of <i>Catharanthus roseus</i> on insulin resistance (IR), oxidative stress and inflammatory responses in dexamethasone (IR inducer)-induced dysfunctional 3T3-L1 adipocytes and high-glucose-induced insulin-resistant L6-myoblast cells. Results showed that dexamethasone-induced dysfunctional 3T3-L1 adipocytes treated with different concentrations of vindoline significantly enhanced basal glucose consumption, accompanied by increased expression of GLUT-4, IRS-1 and adiponectin. Similarly, vindoline-treated insulin-resistant L6 myoblasts exhibited significantly enhanced glycogen content accompanied with upregulation of IRS-1 and GLUT-4. Thus, in vitro studies of vindoline in insulin resistant skeleton muscle and dysfunctional adipocytes confirmed that vindoline treatment significantly mitigated insulin resistance in myotubes and improved functional status of adipocytes. These results demonstrated that vindoline has the potential to be used as a therapeutic agent to ameliorate obesity-induced T2DM-associated insulin resistance profile in adipocytes and skeletal muscles. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T01:32:43Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-e4a25957db8b4a2cbafd438985788cfe2023-11-18T17:13:35ZengMDPI AGNutrients2072-66432023-06-011513286510.3390/nu15132865Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal MyoblastsBeegum Noorjahan Shijina0Achuthan Radhika1Sainulabdeen Sherin2Prabath Gopalakrishnan Biju3Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, IndiaDepartment of Biochemistry, Government College, Kariavattom, Thiruvananthapuram 695581, Kerala, IndiaDepartment of Biochemistry, PMS College of Dental Science and Research, Vattappara, Thiruvananthapuram 695028, Kerala, IndiaDepartment of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695581, Kerala, IndiaType 2 diabetes mellitus (T2DM) emerged as a major health care concern in modern society, primarily due to lifestyle changes and dietary habits. Obesity-induced insulin resistance is considered as the major pathogenic factor in T2DM. In this study, we investigated the effect of vindoline, an indole alkaloid of <i>Catharanthus roseus</i> on insulin resistance (IR), oxidative stress and inflammatory responses in dexamethasone (IR inducer)-induced dysfunctional 3T3-L1 adipocytes and high-glucose-induced insulin-resistant L6-myoblast cells. Results showed that dexamethasone-induced dysfunctional 3T3-L1 adipocytes treated with different concentrations of vindoline significantly enhanced basal glucose consumption, accompanied by increased expression of GLUT-4, IRS-1 and adiponectin. Similarly, vindoline-treated insulin-resistant L6 myoblasts exhibited significantly enhanced glycogen content accompanied with upregulation of IRS-1 and GLUT-4. Thus, in vitro studies of vindoline in insulin resistant skeleton muscle and dysfunctional adipocytes confirmed that vindoline treatment significantly mitigated insulin resistance in myotubes and improved functional status of adipocytes. These results demonstrated that vindoline has the potential to be used as a therapeutic agent to ameliorate obesity-induced T2DM-associated insulin resistance profile in adipocytes and skeletal muscles.https://www.mdpi.com/2072-6643/15/13/2865obesityT2DMinsulin resistancevindolineadipocyteskeletal muscle |
| spellingShingle | Beegum Noorjahan Shijina Achuthan Radhika Sainulabdeen Sherin Prabath Gopalakrishnan Biju Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts Nutrients obesity T2DM insulin resistance vindoline adipocyte skeletal muscle |
| title | Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts |
| title_full | Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts |
| title_fullStr | Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts |
| title_full_unstemmed | Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts |
| title_short | Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts |
| title_sort | vindoline exhibits anti diabetic potential in insulin resistant 3t3 l1 adipocytes and l6 skeletal myoblasts |
| topic | obesity T2DM insulin resistance vindoline adipocyte skeletal muscle |
| url | https://www.mdpi.com/2072-6643/15/13/2865 |
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